LOCUS       sequence-309344-        6774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of 3 copies of: the enhancer 
            for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific 
            NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements,
            plus an additional NF-_B site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pAA1
FEATURES             Location/Qualifiers
     source          1..6774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1911..2051
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1977..1996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2290..2424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2518..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3859..5511
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3925..3943)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5642..5707
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5837..5857
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6282..6416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6689..6707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     1921 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     1981 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2041 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2161 tttcaccgtc atcaccgaaa cgcgcgaggc aggatcagcc atatcacatt tgtagaggtt
     2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2281 attgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2401 aatgtatctt atcatgtctg gatcagccat atcacatttg tagaggtttt acttgcttta
     2461 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttaac
     2521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2641 catgtctgga tccaagcttg cccgggcaga tctctcgagg cgtggctccg tgttgacgca
     2701 tgttgactat gcctttgaat ggctgccgtt cgagacgcgg gaaattccaa cgtgcagaaa
     2761 aaccaacagg gcgctaaaaa ggagagtgtt ttcgtgggag gtggagatgg tcactggggg
     2821 caacataaat attcagcgag aaacgtcata tttacattta gtctaggctg ataatccggg
     2881 gacgcgggaa attccaacgg ggtggtgccg ttggaatttc ccgcgtctcg atcggttatc
     2941 tgcccccggc tgacacttgc ccgtcattca ttcggctgct tatcgcagaa gctccgttgg
     3001 aatttcccgc gtctcgaact cgtttgtctg gtcgaggcgt ggctccgtgt tgacgcatgt
     3061 tgactatgcc tttgaatggc tgccgttcga gacgcgggaa attccaacgt gcagaaaaac
     3121 caacagggcg ctaaaaagga gagtgttttc gtgggaggtg gagatggtca ctgggggcaa
     3181 cataaatatt cagcgagaaa cgtcatattt acatttagtc taggctgata atccggggac
     3241 gcgggaaatt ccaacggggt ggtgccgttg gaatttcccg cgtctcgatc ggttatctgc
     3301 ccccggctga cacttgcccg tcattcattc ggctgcttat cgcagaagct ccgttggaat
     3361 ttcccgcgtc tcgaactcgt ttgtctggtc gaggcgtggc tccgtgttga cgcatgttga
     3421 ctatgccttt gaatggctgc cgttcgagac gcgggaaatt ccaacgtgca gaaaaaccaa
     3481 cagggcgcta aaaaggagag tgttttcgtg ggaggtggag atggtcactg ggggcaacat
     3541 aaatattcag cgagaaacgt catatttaca tttagtctag gctgataatc cggggacgcg
     3601 ggaaattcca acggggtggt gccgttggaa tttcccgcgt ctcgatcggt tatctgcccc
     3661 cggctgacac ttgcccgtca ttcattcggc tgcttatcgc agaagctccg ttggaatttc
     3721 ccgcgtctcg aactcgtttg tctgggactg agctataaaa gcctcaccat ctcaacgctc
     3781 aaagccgttg gaatttcccg cgtccgagct aagatgggag ctcgaattcc agcttggcat
     3841 tccggtactg ttggtaaaat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
     3901 tatcctctag aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     3961 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg
     4021 gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     4081 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     4141 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     4201 ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa
     4261 aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct
     4321 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     4381 ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg
     4441 ataatgaatt cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact
     4501 gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     4561 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     4621 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta
     4681 cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc
     4741 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     4801 gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca
     4861 gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     4921 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     4981 gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga
     5041 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     5101 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata
     5161 gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg
     5221 gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac
     5281 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     5341 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     5401 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     5461 atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta aaatgtaact
     5521 gtattcagcg atgacgaaat tcttagctat tgtaatactc tagaggatct ttgtgaagga
     5581 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     5641 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     5701 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     5761 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     5821 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     5881 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     5941 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     6001 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     6061 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     6121 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     6181 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6241 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcccca
     6421 ggaagctcct ctgtgtcctc ataaacccta acctcctcta cttgagagga cattccaatc
     6481 ataggctgcc catccaccct ctgtgtcctc ctgttaatta ggtcacttaa caaaaaggaa
     6541 attgggtagg ggtttttcac agaccgcttt ctaagggtaa ttttaaaata tctgggaagt
     6601 cccttccact gctgtgttcc agaagtgttg gtaaacagcc cacaaatgtc aacagcagaa
     6661 acatacaagc tgtcagcttt gcacaagggc ctcgtgatac gcctattttt ataggttaat
     6721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtg
//
