LOCUS       sequence-30938-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Leyns L, Bouwmeester T, Kim SH, Piccolo S, De Robertis EM
  TITLE     Frzb-1 is a secreted antagonist of Wnt signaling expressed in the 
            Spemann organizer.
  JOURNAL   Cell. 1997 Mar 21. 88(6):747-56.
  PUBMED    9118218
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1997 
            Mar 21. 88(6):747-56."
COMMENT     Sequence Label: pCS2 xFrzb-HA
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    86..220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(123..142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     177..196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcattggcgc cgcggccgcg aattaaaaaa cctcccacac ctccccctga acctgaaaca
       61 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      181 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tacgtaatac gactcactat
      241 agttctagag gctcgagttt tttttttttt ttatttagga cttaatattt atttaggcaa
      301 tttttaaaac agaattgaca aagtaggcat ttaccttccc aacagtgacc atacagtcta
      361 aaccagctct tcatttatga taaaatattt agatttaggt gcatcctaca tgtcatctaa
      421 ctaaaactgc tgaaagtatt aaatggaaag tgatcatcat ttaaatgtga tctctcactt
      481 gttcatctac aggaaaagca acattggtca acttttctct gatttaccat tgcaattagt
      541 tcactttggc caagtatcaa acacttgtca ccattagttg atcagaaatg aattgagctt
      601 ctttaggctg agacattcag tcactgctct gtcactggaa gttgaataaa acaattgcct
      661 gggaacacgt gcaaatatag ttataaaaaa aaagaaggaa aggagaacta caatcaacta
      721 aaccagcttc ttgtagtaaa caatagaata taacgtgctg tagtgcaatt tcttttttgc
      781 tcttccaaca atgcaaatct tagtttcaaa gtccatagag tttccataca cctttccgtt
      841 agtctaacta cgcgcttgtc tggaattgct gtttttgttg ggaattggag ccacggggtc
      901 tttgcttttc ctgggacgtc gaagcttttg atcccagcgc ttgac
//
