LOCUS       sequence-309382-        7131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of 4 copies of: the enhancer 
            for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific 
            NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements,
            plus an additional NF-_B site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pAA2
FEATURES             Location/Qualifiers
     source          1..7131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1911..2051
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1977..1996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2290..2424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2518..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4216..5868
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4282..4300)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5999..6064
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6194..6214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6639..6773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7046..7064)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     1921 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     1981 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2041 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2161 tttcaccgtc atcaccgaaa cgcgcgaggc aggatcagcc atatcacatt tgtagaggtt
     2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2281 attgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2401 aatgtatctt atcatgtctg gatcagccat atcacatttg tagaggtttt acttgcttta
     2461 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttaac
     2521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2641 catgtctgga tccaagcttg cccgggcaga tctctcgagg cgtggctccg tgttgacgca
     2701 tgttgactat gcctttgaat ggctgccgtt cgagacgcgg gaaattccaa cgtgcagaaa
     2761 aaccaacagg gcgctaaaaa ggagagtgtt ttcgtgggag gtggagatgg tcactggggg
     2821 caacataaat attcagcgag aaacgtcata tttacattta gtctaggctg ataatccggg
     2881 gacgcgggaa attccaacgg ggtggtgccg ttggaatttc ccgcgtctcg atcggttatc
     2941 tgcccccggc tgacacttgc ccgtcattca ttcggctgct tatcgcagaa gctccgttgg
     3001 aatttcccgc gtctcgaact cgtttgtctg gtcgaggcgt ggctccgtgt tgacgcatgt
     3061 tgactatgcc tttgaatggc tgccgttcga gacgcgggaa attccaacgt gcagaaaaac
     3121 caacagggcg ctaaaaagga gagtgttttc gtgggaggtg gagatggtca ctgggggcaa
     3181 cataaatatt cagcgagaaa cgtcatattt acatttagtc taggctgata atccggggac
     3241 gcgggaaatt ccaacggggt ggtgccgttg gaatttcccg cgtctcgatc ggttatctgc
     3301 ccccggctga cacttgcccg tcattcattc ggctgcttat cgcagaagct ccgttggaat
     3361 ttcccgcgtc tcgaactcgt ttgtctggtc gaggcgtggc tccgtgttga cgcatgttga
     3421 ctatgccttt gaatggctgc cgttcgagac gcgggaaatt ccaacgtgca gaaaaaccaa
     3481 cagggcgcta aaaaggagag tgttttcgtg ggaggtggag atggtcactg ggggcaacat
     3541 aaatattcag cgagaaacgt catatttaca tttagtctag gctgataatc cggggacgcg
     3601 ggaaattcca acggggtggt gccgttggaa tttcccgcgt ctcgatcggt tatctgcccc
     3661 cggctgacac ttgcccgtca ttcattcggc tgcttatcgc agaagctccg ttggaatttc
     3721 ccgcgtctcg aactcgtttg tctggtcgag gcgtggctcc gtgttgacgc atgttgacta
     3781 tgcctttgaa tggctgccgt tcgagacgcg ggaaattcca acgtgcagaa aaaccaacag
     3841 ggcgctaaaa aggagagtgt tttcgtggga ggtggagatg gtcactgggg gcaacataaa
     3901 tattcagcga gaaacgtcat atttacattt agtctaggct gataatccgg ggacgcggga
     3961 aattccaacg gggtggtgcc gttggaattt cccgcgtctc gatcggttat ctgcccccgg
     4021 ctgacacttg cccgtcattc attcggctgc ttatcgcaga agctccgttg gaatttcccg
     4081 cgtctcgaac tcgtttgtct gggactgagc tataaaagcc tcaccatctc aacgctcaaa
     4141 gccgttggaa tttcccgcgt ccgagctaag atgggagctc gaattccagc ttggcattcc
     4201 ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     4261 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     4321 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
     4381 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     4441 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     4501 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     4561 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
     4621 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
     4681 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     4741 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
     4801 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
     4861 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     4921 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     4981 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
     5041 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
     5101 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     5161 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     5221 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     5281 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     5341 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     5401 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     5461 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     5521 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     5581 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     5641 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     5701 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     5761 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     5821 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     5881 ttcagcgatg acgaaattct tagctattgt aatactctag aggatctttg tgaaggaacc
     5941 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     6001 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     6061 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     6121 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     6181 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     6241 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     6301 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     6361 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     6421 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     6481 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     6541 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6601 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccccagga
     6781 agctcctctg tgtcctcata aaccctaacc tcctctactt gagaggacat tccaatcata
     6841 ggctgcccat ccaccctctg tgtcctcctg ttaattaggt cacttaacaa aaaggaaatt
     6901 gggtaggggt ttttcacaga ccgctttcta agggtaattt taaaatatct gggaagtccc
     6961 ttccactgct gtgttccaga agtgttggta aacagcccac aaatgtcaac agcagaaaca
     7021 tacaagctgt cagctttgca caagggcctc gtgatacgcc tatttttata ggttaatgtc
     7081 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt g
//
