LOCUS       sequence-309387-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AscI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG714_lexO-AscI_LexA-TF_URA3MX
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(67..316)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(366..389)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(396..416)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2198..2217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2199..2217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2224..2241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2224..2240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2233..2255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2343..2686
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3505..3702
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        4067..4171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4172..4240,4241..5032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4390..4409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5211..5799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5700..5719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5953..5970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6280..6296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6285..6307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6304..6320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6304..6320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6341..6359
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(6522..6771)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(7235..8125)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(7238..8173)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(join(8192..8545,8546..8797))
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     complement(8233..8254)
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 ctactaagga aaacaacaac ggaagcgatg atgagagcga cgatgagagt gtaaactgcg
       61 aaccttgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg ttttcagtat
      121 aatgttacat gcgtacacgc gtttgtacag aaaaaaaaga aaaatttgaa atataaataa
      181 cgttcttaat actaacataa ctattaaaaa aaataaatag ggacctagac ttcaggttgt
      241 ctaactcctt ccttttcggt tagagcggat gtgggaggag ggcgtgaatg taagcgtgac
      301 ataactaatt acatgatata gacaaaggaa aaggggcctg tctcgaggtt agttagttaa
      361 agcttcttgt cgtcgtcatc cttgtagtcg atatcaactt ttctcttctt ctttggagat
      421 gggtccaaaa ctatttctgg tctagcaatc cacttatcca acaaggcttc caaatcacca
      481 ttagccaact ttctttcgac agcacccaaa tcaaagattt cttgcagcaa tgggtcattc
      541 aacaaagggt cattgaaagc caaaatagca tcgtacaagt gtctcaaaga accttcagca
      601 attctgtact caccttcttc caaagtgaaa tctctaccat gaccccatct aacatcagac
      661 aaaccatcga tgtaacctgg cctcaaaaac aaaggatcta agttagcaac caaagcacct
      721 ctaactggaa ctgatgcagc atcatctctg taataggaac cgaacttcaa caactggtaa
      781 gtacctttct taacatcatc ggttctaccc aaacctggtt ttgttttgtt agaatcgatt
      841 tcgtcacccc aaccattaac caaataagcc aaagcagcag ttctaccagc tctttgagtc
      901 ttgggagttc tgtaagcagc atataattca atccaggctt gcaagtaaga agcggcatta
      961 gctggaacag aaaagtaatc aatcaatggt tcgtatggcc aagattcgcc ttctctttca
     1021 cccaatggaa tagctgtttc agcatgtgga atagcaacag caaattctct accctgcaac
     1081 gtcaagtcaa ccaaagaatg ttgtctatac tcaactggac cgtctggacc ttcttgcaac
     1141 ataggttctt ccaattcagt aaccaatggg aaagaaacca ttggagaagc cttgatttcg
     1201 aacaagacca acaagtcatc tctgaaacca atagcatcaa catcatggga ttgcttggtg
     1261 atagtagcaa tttcgaatgg gttttcgtta gcagcagcaa ctaattgcaa caacaaggca
     1321 atagcaacgg tggtaatttc accaatatga tgagaagttg gcttatgttg agcaccagac
     1381 aatctacctt ccaaacctct atccaagcag catcttggac attgtttcaa gaaagagtaa
     1441 taagcaactg ctggttcacc acctctgtga caatagatcc acttgttgtt aaccaatcta
     1501 ccgtgaacgt attcagcgtt acagatcaaa tcgaaaccag cagacaacat caaaccgaac
     1561 aacttagctt catcatgagc ttcaaccaaa ttagctctag tagcagattc ttcagcagaa
     1621 ccaataacgg atctgaagga tctgatgaaa gtacccaata atctagcagc gttagcacca
     1681 tcaacatatt gagagttggt caaagacctg tccatcaatc tttccaaatc ggagattttt
     1741 ggagcagcag aagaatctct gtactctgga aattcaatca tgaattcctg cagcccgggg
     1801 gatccactag tatagaagta tagtaattta tgctgcaaag gtcctaatgt ataaggaaag
     1861 aagagaaaag aagaaaacaa gagttttata tacatacaga gcacatgctc tagaccctgg
     1921 gtgtatatac agttatgctg tgagtatata cagcacctag accctgggtg tatatacagt
     1981 tatgctgtga gtatatacag cacctagacc ctgggtgtat atacagttat gctgtgagta
     2041 tatacagcac ctagaccctg ggtgtatata cagttatgct gtgagtatat acagcaccta
     2101 gggagctcca attcgcccta taggcggccg cgaaaagaaa tatagtcttc aaggttatat
     2161 tatacaggtt tatatattat tctgtttctc caattcgccc tatagtgagt cgtattacaa
     2221 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     2281 tcgccttgca gcacatccga tcggtttagc ttgcctcgtc cccgccgggt cacccggcca
     2341 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
     2401 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
     2461 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg
     2521 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
     2581 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
     2641 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatga cagtcaacac
     2701 taagacctat agtgagagag cagaaactca tgcctcacca gtagcacaac gattatttcg
     2761 attaatggaa ctgaagaaaa ccaatttatg tgcatcaatt gatgttgata ccactaagga
     2821 atttcttgaa ttaattgata aattgggtcc ttatgtatgc ttaatcaaga ctcatattga
     2881 tataatcaat gatttttcct atgaatccac tattgaacca ttattagaac tttcacgtaa
     2941 acatcaattt atgatttttg aagatagaaa atttgctgat attggtaata ccgtgaagaa
     3001 acaatatatt ggtggagttt ataaaattag tagttgggca gatattacta atgctcatgg
     3061 tgtcactggg aatggagtag ttgaaggatt aaaacaggga gctaaagaaa ccaccaccaa
     3121 ccaagagcca agagggttat tgatgttagc tgaattatca tcagtgggat cattagctta
     3181 tggagaatat tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat ttgttattgg
     3241 atttattgcc caacgtgata tgggtggaca agaagaagga tttgattggc ttattatgac
     3301 acctggagtt ggattagatg ataaaggtga tggattagga caacaatata gaactgttga
     3361 tgaagttgtt agcactggaa ctgatattat cattgttggt agaggattgt ttggtaaagg
     3421 aagagatcca gatattgaag gtaaaaggta tagagatgct ggttggaatg cttatttgaa
     3481 aaagactggc caattataat cagtactgac aataaaaaga ttcttgtttt caagaacttg
     3541 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     3601 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     3661 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     3721 cgccgccatc cagtgtcgaa aacgcatatg ccgggaatct cggtcgtaat gatttctata
     3781 atgacgaaaa aaaaaaaatt ggaaagaaaa accttcatgg cctttataaa aaggaactat
     3841 ccaatacctc gccagaacca agtaacagta ttttacgggg cacaaatcaa gaacaataag
     3901 acaggactgt aaagatggac gcattgaact ccaaagaaca acaagagttc caaaaagtag
     3961 tggcgcgcca gagttaatta atagcagttc gcaaaatcgt tcgcttcgca aatgttgcta
     4021 atgccgcgtt ctttcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4561 cactgcggcc aacttacttc tgacaaccat cggaggaccg aaggagctaa ccgctttttt
     4621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4861 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5161 ccactgagcg tcagaccccg taggtttaaa caaaagatca aaggatcttc ttgagatcct
     5221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5341 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6001 cctctccccg cgcgttggcc gattcattaa tgcctgcagg gagaggcgcg ccaattgatg
     6061 acaatacaga cgatgataac aaaccgaagt tatctgatgt agaaaaggat taaagatgct
     6121 aagagatagt gatgatattt cataaataat gtaattctat atatgttaat tacctttttt
     6181 gcgaggcata tttatggtga aggataagtt ttgaccatca aagaaggtta atgtggctgt
     6241 ggtttcaggg tccataaagc tattcaattc atccagctgt tgtgagcgga taacaatttc
     6301 acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct cactaaaggg
     6361 aacaaaagct gggtacaagt gacaatatat atatatatat ataataacga catcgttatc
     6421 tgtaaatacg gccgaagctg ggtaccaagc agacgctact aaggaaaaca acaacggaag
     6481 cgatgatgag agcgacgatg agagtgtaaa ctgcgaacct tgcaaattaa agccttcgag
     6541 cgtcccaaaa ccttctcaag caaggttttc agtataatgt tacatgcgta cacgcgtttg
     6601 tacagaaaaa aaagaaaaat ttgaaatata aataacgttc ttaatactaa cataactatt
     6661 aaaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     6721 cggatgtggg aggagggcgt gaatgtaagc gtgacataac taattacatg atatagacaa
     6781 aggaaaaggg gcctgtctcg aggataagct ttaaacgcgt ttaaagcttg aaacacaaat
     6841 cagtaccacc agaatgtctt tcagctctaa attcaccaga ggatcttctt tcagcaactg
     6901 gatcgaaatc atcagcagat ggaatagctt cttcgaattt ttccaaacca gaatcgaaat
     6961 taactttagc atctttaaca gtcaaagttt cccaaatcaa atcagattgt tgttccaaaa
     7021 caccaatagc ttcacccaaa gaagcaacag cagtatccaa tttattaaca gaacctggag
     7081 catcgaaaac caattcagca ataccatctt ccaaccaatc caaatacaaa gtatcacctt
     7141 tatacaacat atcagtttct tggattctca aagtaatacc tggaaattcg cccgcggtgc
     7201 tgttatcgtt gcccggcaga tcctgcatga tatcgactgt ggcagggaaa ccctctgcct
     7261 cccccgtgat gtaatacttt tgcaaggaat gcgatgaagt agagcccgca gtggccaagt
     7321 ggctttggtc cgtctcctcc acggatgccc ctccacggct agtgggcgca tgtaggcggt
     7381 gggcgtccag catctccagc agcaggtcat agaggggcac cacgttcttg cacttcatgc
     7441 tgtacagatg ctccatgcct ttgttactca tgtgcctgat gtgggagagg atgaggagga
     7501 gctgggccag ccgctggtgc tgctgctgca gggtcaggcc tgccttggcc atcaggtgga
     7561 tcaaagtgtc tgtgatcttg tccaggactc ggtggatatg gtccttctct tccagagact
     7621 tcagggtgct ggacagaaat gtgtacactc cagaattaag caaaataata gatttgaggc
     7681 acacaaactc ctctccctgc agattcatca tgcggaaccg agatgatgta gccagcagca
     7741 tgtcgaagat ctccaccatg ccctctacac attttccctg gttcctgtcc aagagcaagt
     7801 taggagcaaa cagtagcttc actgggtgct ccatggagcg ccagacgaga ccaatcatca
     7861 ggatctctaa ccaggcacat tctagaaggt ggacctgatc atggagggtc aaatccacaa
     7921 agcctggcac cctcttcgcc cagttgatca tgtgaaccag ctccctgtct gccaggttgg
     7981 tcagtaagcc catcatcgaa gcttcactga agggtctggt aggatcatac tcggaataga
     8041 gtatgggggg ctcagcatcc aacaaggcac tgaccatctg gtcggccgtc agggacaagg
     8101 ccaggctgtt cttcttagag cgtttgatca tgagcgggct tggccaaagg ttggcagctc
     8161 tcatgtctcc agcagagata tccttagaac ccagccagtc gccgttgcga ataaccccaa
     8221 ccgccagccc ttcaatggtg aagctctgct gacgaagatc tacgacaatt ggtttaaact
     8281 cgctattttc tggcaacagt tcgactttat tgccctgttt tttcaggcgc ttaacggtaa
     8341 cctcgtcatc aatacgtgcg acaacgacct gaccgttacg tacatcctga gttttatgca
     8401 ctgccagcaa gtcaccatcc ataatgccga tgtctttcat cgacatcccg ctgacgcgca
     8461 gcaggaaatc agcattcggc ttgaataagg aaggatcgac ctgataatga ccttcaatat
     8521 gctgttgcgc cagaagtggt tcaccggcag ccacacgacc taccagcggc aacccttctt
     8581 cctcttcctg caacagacga atcccgcgtg atgcgccgga aacaatttca ataacgcctt
     8641 tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg ggaacggaac cccaaacgct
     8701 gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct gatgtgatca cggatgagat
     8761 caaacacctc ttgttgcctg gccgttaacg ctttcatgaa ttcctgcagc ccgggggatc
     8821 cactagttgt taattcagta aattttcgat cttgggaaga aaaaagcagt aagcgtgaaa
     8881 aatctaaaag ctgatgtagt agaagatcct attctttaac aaagattgac cttttctttt
     8941 tcttcttggt ttgagtagaa aggggaagga agaatacaag agagaggaaa aaaaggaaga
     9001 taaaaagaga gcgtgatata aatgaatata tattaaacaa gagagattgg gaaggaaagg
     9061 atcaaacaaa cccaaaaata tttcaaaaag gagagagaga ggcgagtttg gtttcaaaac
     9121 ggtttattta tttatgcaag aggacgtgga agaaaaagaa gaaggaagaa aaaaatttga
     9181 aagaaaaaaa cgcgtggcgg gtaaagaaga aaatggaaaa tagaggccgg gtgacagaga
     9241 aatattgagg gttaattgga aaatatgtta gggtgaggca tatgttttta agggttttga
     9301 ggatccgata aggaagaatg taggttaaat gttgtgcatt aattgctgtg gcagcttacc
     9361 cgcttctcta gagcggccga agctgggtac caagcagacg
//
