LOCUS       sequence-309388-       10159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AscI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 10159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG645_lexO-AscI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..10159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1790..1810
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1817..1840
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1890..2139
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(2809..3060,3061..3414)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3352..3373
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3433..4368
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3481..4371
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      4835..5084
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5247..5265)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5286..5302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5286..5302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5299..5321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5310..5326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(5585..5602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5756..6344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5836..5855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6523..7314,7315..7383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7146..7165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7384..7488)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10110..10132
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10124..10141
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10125..10141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10148..10159,1..8)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10148..10159,1..7)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatagggcg aattggagaa acagaataat atataaacct gtataatata accttgaaga
       61 ctatatttct tttcgcggcc gcctataggg cgaattggag ctccctaggt gctgtatata
      121 ctcacagcat aactgtatat acacccaggg tctaggtgct gtatatactc acagcataac
      181 tgtatataca cccagggtct aggtgctgta tatactcaca gcataactgt atatacaccc
      241 agggtctagg tgctgtatat actcacagca taactgtata tacacccagg gtctagagca
      301 tgtgctctgt atgtatataa aactcttgtt ttcttctttt ctcttctttc cttatacatt
      361 aggacctttg cagcataaat tactatactt ctatactagt ggatcccccg ggctgcagga
      421 attcatgatt gaatttccag agtacagaga ttcttctgct gctccaaaaa tctccgattt
      481 ggaaagattg atggacaggt ctttgaccaa ctctcaatat gttgatggtg ctaacgctgc
      541 tagattattg ggtactttca tcagatcctt cagatccgtt attggttctg ctgaagaatc
      601 tgctactaga gctaatttgg ttgaagctca tgatgaagct aagttgttcg gtttgatgtt
      661 gtctgctggt ttcgatttga tctgtaacgc tgaatacgtt cacggtagat tggttaacaa
      721 caagtggatc tattgtcaca gaggtggtga accagcagtt gcttattact ctttcttgaa
      781 acaatgtcca agatgctgct tggatagagg tttggaaggt agattgtctg gtgctcaaca
      841 taagccaact tctcatcata ttggtgaaat taccaccgtt gctattgcct tgttgttgca
      901 attagttgct gctgctaacg aaaacccatt cgaaattgct actatcacca agcaatccca
      961 tgatgttgat gctattggtt tcagagatga cttgttggtc ttgttcgaaa tcaaggcttc
     1021 tccaatggtt tctttcccat tggttactga attggaagaa cctatgttgc aagaaggtcc
     1081 agacggtcca gttgagtata gacaacattc tttggttgac ttgacgttgc agggtagaga
     1141 atttgctgtt gctattccac atgctgaaac agctattcca ttgggtgaaa gagaaggcga
     1201 atcttggcca tacgaaccat tgattgatta cttttctgtt ccagctaatg ccgcttctta
     1261 cttgcaagcc tggattgaat tatatgctgc ttacagaact cccaagactc aaagagctgg
     1321 tagaactgct gctttggctt atttggttaa tggttggggt gacgaaatcg attctaacaa
     1381 aacaaaacca ggtttgggta gaaccgatga tgttaagaaa ggtacttacc agttgttgaa
     1441 gttcggttcc tattacagag atgatgctgc atcagttcca gttagaggtg ctttggttgc
     1501 taacttagat cctttgtttt tgaggccagg ttacatcgat ggtttgtctg atgttagatg
     1561 gggtcatggt agagatttca ctttggaaga aggtgagtac agaattgctg aaggttcttt
     1621 gagacacttg tacgatgcta ttttggcttt caatgaccct ttgttgaatg acccattgct
     1681 gcaagaaatc tttgatttgg gtgctgtcga aagaaagttg gctaatggtg atttggaagc
     1741 cttgttggat aagtggattg ctagaccaga aatagttttg gacccatctc caaagaagaa
     1801 gagaaaagtt gatatcgact acaaggatga cgacgacaag aagctttaac taactaacct
     1861 cgagacaggc cccttttcct ttgtctatat catgtaatta gttatgtcac gcttacattc
     1921 acgccctcct cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
     1981 ggtccctatt tatttttttt aatagttatg ttagtattaa gaacgttatt tatatttcaa
     2041 atttttcttt tttttctgta caaacgcgtg tacgcatgta acattatact gaaaaccttg
     2101 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca aggttcgcag tttacactct
     2161 catcgtcgct ctcatcatcg cttccgttgt tgttttcctt agtagcgtct gcttggtacc
     2221 cagcttcggc cgctctagag aagcgggtaa gctgccacag caattaatgc acaacattta
     2281 acctacattc ttccttatcg gatcctcaaa acccttaaaa acatatgcct caccctaaca
     2341 tattttccaa ttaaccctca atatttctct gtcacccggc ctctattttc cattttcttc
     2401 tttacccgcc acgcgttttt ttctttcaaa tttttttctt ccttcttctt tttcttccac
     2461 gtcctcttgc ataaataaat aaaccgtttt gaaaccaaac tcgcctctct ctctcctttt
     2521 tgaaatattt ttgggtttgt ttgatccttt ccttcccaat ctctcttgtt taatatatat
     2581 tcatttatat cacgctctct ttttatcttc ctttttttcc tctctcttgt attcttcctt
     2641 cccctttcta ctcaaaccaa gaagaaaaag aaaaggtcaa tctttgttaa agaataggat
     2701 cttctactac atcagctttt agatttttca cgcttactgc ttttttcttc ccaagatcga
     2761 aaatttactg aattaacaac tagtggatcc cccgggctgc aggaattcat gaaagcgtta
     2821 acggccaggc aacaagaggt gtttgatctc atccgtgatc acatcagcca gacaggtatg
     2881 ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa
     2941 gaacatctga aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc
     3001 gggattcgtc tgttgcagga agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc
     3061 ggtgaaccac ttctggcgca acagcatatt gaaggtcatt atcaggtcga tccttcctta
     3121 ttcaagccga atgctgattt cctgctgcgc gtcagcggga tgtcgatgaa agacatcggc
     3181 attatggatg gtgacttgct ggcagtgcat aaaactcagg atgtacgtaa cggtcaggtc
     3241 gttgtcgcac gtattgatga cgaggttacc gttaagcgcc tgaaaaaaca gggcaataaa
     3301 gtcgaactgt tgccagaaaa tagcgagttt aaaccaattg tcgtagatct tcgtcagcag
     3361 agcttcacca ttgaagggct ggcggttggg gttattcgca acggcgactg gctgggttct
     3421 aaggatatct ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc
     3481 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc
     3541 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt
     3601 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc
     3661 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt
     3721 ctagaatgtg cctggttaga gatcctgatg attggtctcg tctggcgctc catggagcac
     3781 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta
     3841 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg
     3901 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg
     3961 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc
     4021 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag
     4081 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt
     4141 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac
     4201 ctgctgctgg agatgctgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca
     4261 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc
     4321 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagt cgatatcatg
     4381 caggatctgc cgggcaacga taacagcacc gcgggcgaat ttccaggtat tactttgaga
     4441 atccaagaaa ctgatatgtt gtataaaggt gatactttgt atttggattg gttggaagat
     4501 ggtattgctg aattggtttt cgatgctcca ggttctgtta ataaattgga tactgctgtt
     4561 gcttctttgg gtgaagctat tggtgttttg gaacaacaat ctgatttgat ttgggaaact
     4621 ttgactgtta aagatgctaa agttaatttc gattctggtt tggaaaaatt cgaagaagct
     4681 attccatctg ctgatgattt cgatccagtt gctgaaagaa gatcctctgg tgaatttaga
     4741 gctgaaagac attctggtgg tactgatttg tgtttcaagc tttaaacgcg tttaaagctt
     4801 atcctcgaga caggcccctt ttcctttgtc tatatcatgt aattagttat gtcacgctta
     4861 cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag acaacctgaa
     4921 gtctaggtcc ctatttattt tttttaatag ttatgttagt attaagaacg ttatttatat
     4981 ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa
     5041 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaaggtt cgcagtttac
     5101 actctcatcg tcgctctcat catcgcttcc gttgttgttt tccttagtag cgtctgcttg
     5161 gtacccagct tcggccgtat ttacagataa cgatgtcgtt attatatata tatatatata
     5221 ttgtcacttg tacccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     5281 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaacagc tgtaatatat
     5341 agtagtaacc tgaaaaatag tcaatgcccg tggttcattt gccaatagaa cagtcgatgt
     5401 gatcaatgta cacgaaagtc aatataaaat atccaaatag ccaatggtgt caggacggct
     5461 aaggcaaatg caactttgaa atacttattc ggtttgcatc ttggcgcgcc tcttcctgca
     5521 ggcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6181 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6361 tttgtttaaa cctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6961 tccgatggtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7501 aaaagtgcca cctgacgtcg aaagaacgcg gcattagcaa catttgcgaa gcgaacgatt
     7561 ttgcgaactg ctattaatta actctggcgc gcctaacttt ttgtgtggtg ccctcctcct
     7621 tgtcaatatt aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca
     7681 atttgcttac ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag
     7741 attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgca
     7801 tatgatccgc aggctaaccg gaacctgtat tatttagttt atgctacgtt aaataaagac
     7861 ctttcgttca cataactgaa tgtgtaatgg ccttgagatt tcaagcatac caagttggtg
     7921 gagacggggt cgttacaaaa gactctttaa gccaagattt ccttgacagc cttggcgata
     7981 gcatcgccaa cctcagtggt agagttagaa ccaccaaggt caccggttct gacacctgca
     8041 tccaagacat ttctaacagc ttcttcaaga gccctacctt cttcaaccaa atccaaggat
     8101 aacttcaaca tcatagctgc agataagatg gtagcaattg ggttaacctt gtttgctggt
     8161 aaatctgggg cagaaccatg acatggttcg tacaaaccga atgccttgtt agtgtcaggt
     8221 agggaagcta gagatgcaga aggtaataaa cccaaagaac ctggaataac agaggcttca
     8281 tcggagataa tatcaccaaa catgttgttg gtaataacaa caccgtttag cttagttggt
     8341 gatttaacca aaatcatagc agcagagtcg atcaattggt gctgaacagt taattgtggg
     8401 aactcagtct tgatggtttc ttcaacagtc tttctccaca atctggaaga ggcaagcacg
     8461 ttagccttgt caagagacca gattggtaat ggtgggtttt gttgcaatgc caagaaagca
     8521 gccattcttg taattctttg aacttcagga acactgtatt tctcagagtc ccaagcaact
     8581 ccgtcacctt catcttcttt tctttcacca aagtagatac caccaaccaa ttctctaacg
     8641 acgacgaaat cagtaccctt tgcatattca ggcttcaaag gagaaagatc tagtaaagaa
     8701 tcagaagcaa agttacatgg tctcaagttg gcgtatagac ccaattcctt tctgatcttc
     8761 aatagacctt gttctggtct aactgcgccc gtaccccatt ttggaccacc aacagcacct
     8821 agtaagacag catcggcttt cttagaggct tcaagagctt catctggtaa aggagtgcca
     8881 gtggcatcga tggcagcacc cccgatcaag tgatgttgga aattgaactt aatttctgga
     8941 cggacttcag caatggcatt caagacctta atagcttcgt cagtaacttc tttaccgacg
     9001 tgatcacccg gtaggacaac gatattctta gacatggtat aatctgtgta gtgtgggata
     9061 ctttttactt cttcaaatag gtatcaacaa taaaaataaa tcgaagcaaa tgtaggaatg
     9121 cgttaaagca gatgtactta cttagagtac atagatatat ttatataatt caatatataa
     9181 aagtatatga agcatctgat gttgaacctg tcatgactct aaactggaat gggtagttat
     9241 gcttctgaag gttttccgtt agaaaatgag tgcagggatc aggcccggaa ccggttttag
     9301 cctgaaagga aaagaaaaaa atgcggtgat tactagcacg tgactgcgct gaattggaat
     9361 ctaccacaag tacagcgaat ggcaagaggg aaggggaata tattgtgcct ggcggtagcg
     9421 tctgctatat caaatgttaa acataactaa atgcagtagt gaatagagta atgtatttct
     9481 acatctgtgg gtagggccaa tgggagcccg atgtctattg tacagatatt ttccttttat
     9541 agttaatcaa ttcaacttgg aaatcattga gtcgatgtct acttggataa ctggagccgt
     9601 ggcagcgttt gaaatttgac gagtcttctt gttttgttcg cgcattttca gtttattcaa
     9661 acgggagatg gcaccgaatc cactgacttc tctgttcaat ttgaatggta ataggaagaa
     9721 agtacatttc gagaaatttt cgcccaacgt cgaaatctca tggatgattt cctcgataca
     9781 aatgacacct tcatcaccta atttctcttc aacgatattg ttgtcattca agattatctc
     9841 ttttggttct ttatcttctg gcctttgcca cattattcta gttctctttt ggattaaaga
     9901 acggatagat gcaagtgaag gttgtccgat aacaatatat ggtgatacaa gtttcaacaa
     9961 tgggtacacg tccttggtaa gtttgatgaa aacaccagtg ttcaactcaa caagtctcaa
    10021 aagagataac actttgtaag cctttgctgg gatcttcaca gcgatcggat gtgctgcaag
    10081 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10141 tgaattgtaa tacgactca
//
