LOCUS       sequence-309398-        7488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase under the control of 5 copies of: the enhancer 
            for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific 
            NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements,
            plus an additional NF-_B site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pAA3
FEATURES             Location/Qualifiers
     source          1..7488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1911..2051
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1977..1996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2290..2424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2518..2652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4573..6225
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4639..4657)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          6356..6421
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6551..6571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6996..7130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7403..7421)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     1921 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     1981 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2041 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2161 tttcaccgtc atcaccgaaa cgcgcgaggc aggatcagcc atatcacatt tgtagaggtt
     2221 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2281 attgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2401 aatgtatctt atcatgtctg gatcagccat atcacatttg tagaggtttt acttgcttta
     2461 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttaac
     2521 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2581 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2641 catgtctgga tccaagcttg cccgggcaga tctctcgagg cgtggctccg tgttgacgca
     2701 tgttgactat gcctttgaat ggctgccgtt cgagacgcgg gaaattccaa cgtgcagaaa
     2761 aaccaacagg gcgctaaaaa ggagagtgtt ttcgtgggag gtggagatgg tcactggggg
     2821 caacataaat attcagcgag aaacgtcata tttacattta gtctaggctg ataatccggg
     2881 gacgcgggaa attccaacgg ggtggtgccg ttggaatttc ccgcgtctcg atcggttatc
     2941 tgcccccggc tgacacttgc ccgtcattca ttcggctgct tatcgcagaa gctccgttgg
     3001 aatttcccgc gtctcgaact cgtttgtctg gtcgaggcgt ggctccgtgt tgacgcatgt
     3061 tgactatgcc tttgaatggc tgccgttcga gacgcgggaa attccaacgt gcagaaaaac
     3121 caacagggcg ctaaaaagga gagtgttttc gtgggaggtg gagatggtca ctgggggcaa
     3181 cataaatatt cagcgagaaa cgtcatattt acatttagtc taggctgata atccggggac
     3241 gcgggaaatt ccaacggggt ggtgccgttg gaatttcccg cgtctcgatc ggttatctgc
     3301 ccccggctga cacttgcccg tcattcattc ggctgcttat cgcagaagct ccgttggaat
     3361 ttcccgcgtc tcgaactcgt ttgtctggtc gaggcgtggc tccgtgttga cgcatgttga
     3421 ctatgccttt gaatggctgc cgttcgagac gcgggaaatt ccaacgtgca gaaaaaccaa
     3481 cagggcgcta aaaaggagag tgttttcgtg ggaggtggag atggtcactg ggggcaacat
     3541 aaatattcag cgagaaacgt catatttaca tttagtctag gctgataatc cggggacgcg
     3601 ggaaattcca acggggtggt gccgttggaa tttcccgcgt ctcgatcggt tatctgcccc
     3661 cggctgacac ttgcccgtca ttcattcggc tgcttatcgc agaagctccg ttggaatttc
     3721 ccgcgtctcg aactcgtttg tctggtcgag gcgtggctcc gtgttgacgc atgttgacta
     3781 tgcctttgaa tggctgccgt tcgagacgcg ggaaattcca acgtgcagaa aaaccaacag
     3841 ggcgctaaaa aggagagtgt tttcgtggga ggtggagatg gtcactgggg gcaacataaa
     3901 tattcagcga gaaacgtcat atttacattt agtctaggct gataatccgg ggacgcggga
     3961 aattccaacg gggtggtgcc gttggaattt cccgcgtctc gatcggttat ctgcccccgg
     4021 ctgacacttg cccgtcattc attcggctgc ttatcgcaga agctccgttg gaatttcccg
     4081 cgtctcgaac tcgtttgtct ggtcgaggcg tggctccgtg ttgacgcatg ttgactatgc
     4141 ctttgaatgg ctgccgttcg agacgcggga aattccaacg tgcagaaaaa ccaacagggc
     4201 gctaaaaagg agagtgtttt cgtgggaggt ggagatggtc actgggggca acataaatat
     4261 tcagcgagaa acgtcatatt tacatttagt ctaggctgat aatccgggga cgcgggaaat
     4321 tccaacgggg tggtgccgtt ggaatttccc gcgtctcgat cggttatctg cccccggctg
     4381 acacttgccc gtcattcatt cggctgctta tcgcagaagc tccgttggaa tttcccgcgt
     4441 ctcgaactcg tttgtctggg actgagctat aaaagcctca ccatctcaac gctcaaagcc
     4501 gttggaattt cccgcgtccg agctaagatg ggagctcgaa ttccagcttg gcattccggt
     4561 actgttggta aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatcct
     4621 ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     4681 cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac
     4741 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     4801 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     4861 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     4921 agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt
     4981 ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg
     5041 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     5101 aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg
     5161 aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc
     5221 gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     5281 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     5341 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc
     5401 cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc
     5461 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc
     5521 gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata
     5581 cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     5641 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     5701 gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg
     5761 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     5821 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catagttgac
     5881 cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg
     5941 atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc cgacgatgac
     6001 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     6061 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     6121 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     6181 gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaaatgt aactgtattc
     6241 agcgatgacg aaattcttag ctattgtaat actctagagg atctttgtga aggaacctta
     6301 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     6361 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     6421 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     6481 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     6541 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     6601 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     6661 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     6721 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     6781 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     6841 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     6901 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6961 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     7021 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7081 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccaggaagc
     7141 tcctctgtgt cctcataaac cctaacctcc tctacttgag aggacattcc aatcataggc
     7201 tgcccatcca ccctctgtgt cctcctgtta attaggtcac ttaacaaaaa ggaaattggg
     7261 taggggtttt tcacagaccg ctttctaagg gtaattttaa aatatctggg aagtcccttc
     7321 cactgctgtg ttccagaagt gttggtaaac agcccacaaa tgtcaacagc agaaacatac
     7381 aagctgtcag ctttgcacaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7441 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtg
//
