LOCUS       sequence-309405-        6606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter 
            and hr5 enhancer from Autographa californica nuclear polyhedris 
            virus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pKM5
FEATURES             Location/Qualifiers
     source          1..6606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             1087..1110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4123..4430
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        4460..4564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4565..4633,4634..5425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4783..4802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5596..6184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6085..6104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6338..6355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6472..6493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6508..6513,6514..6531,6532..6538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6546..6562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6551..6573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6570..6586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6570..6586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatggatt acaaggatga cgatgacaag tcaacactac tcaatttgga tagctaccgg
     1141 catgagttat tcgagcacca tcagcatcag cagcagcatc accagctcca gcagctttcg
     1201 tcatcgccga cgtattcggt gctctcgatg gaatctgtat cgcccgtgtc gtcatcatcg
     1261 gcaagccccg cagccgacac tagcaaatac tacgcgagca actctccgtc cagtgtcagt
     1321 aatatgtccc ccaaatcaac agctagtgat acgagctcct tcaacatgca gaatctgaac
     1381 atctccaacg cctacccgta ctttgccgag actcatcatc agacgatgac aaccggcgaa
     1441 gagggtcaac tgcagacctt tacctataat gatccaaccc agcctcgagt ggagctttcg
     1501 gtacctcagc taacgatcgt tgagcaaccg gtggacaaat tccgcttccg ataccagtcg
     1561 gagatgcacg gaacacatgg gtccctgatg ggggtccata cggagaaaag caagaaaacc
     1621 ttccccaccg tgcagctgca tgggtttcag ggcgaggcca agattcgatg ttcgctgttc
     1681 caggtggacc cgaaccggag ggctgcccat tcgcacaacc tggtgatcaa gagtggcgaa
     1741 atagatttga acgatccgca tgacatcgat gtgaacgaag agtgcaacta tgtggccatg
     1801 ttccagggga tgggcatcat acacacggcc aaaaagaata tcgccgagga gttgagcaag
     1861 aagatgaaga agcaacggtc ggtggagatg aaccgggagc tgaccctcag ggaagagtac
     1921 cagttgcaga aggaagcggc cgaaatggcc aagacgatga atctgaatca ggtgtgcctg
     1981 tgcttccagg cgttccaggt ggatgcctcg accggtcggt gggcgcagct ctgcgaaccg
     2041 gtttattcca atccgattaa taatatgaag agtgccctca cgggggaatt gaagatctgc
     2101 cggctcagtg ctaccgtggg aaacgttgac ggcggcgaag aggtgttcat gttcgttgaa
     2161 aaagtttgca aaaataacat caagatccgg ttctatgaac tggacgaata cgatcaggag
     2221 atctggcaag actggggttc cttctcggaa gctgacgtcc atcatcaata tgcgatcgca
     2281 ttcaaaactc ccgcttatca caacaaagac atcaccgaac cggtggaagt gctcatgcag
     2341 ctctaccggc cacgagacaa atgccagagc gaacccgtcc cgttcaaatt caagccccgg
     2401 ttcaacatat ctcggaagcg cccgagagtc agttccggct tactttcgaa tgagattcca
     2461 acagtagttc ccaatgaacc gggatcgagc cgacttccgt cgttccatca tccgttttcc
     2521 atgatgcccc cgatgcaagc gcccatcgcc gaggccagtg gaagcagcag tagccacacc
     2581 atcagcaaag agtacaacaa atccggtatc atccaggaga ttttggacag ccacattccg
     2641 accacaattg ccggcgatat atcgttcagc tcgaacgact tcaaagaatt cgtcaattgc
     2701 aactcggaag aattacgcaa gctaataacg gagatcgggg aagcgcagga ggagtccaag
     2761 ctggagacgg acgcggtatc ggttggtcac gtggacgagg cagcggcgca gtttgagcgc
     2821 acgctagagc agtacctgga gcacaacagc caagccaagg acgtggagat tctgaagaag
     2881 atactggcca tcatacggtt actgttccgg aagaactacg accagtgccg ggaacttatc
     2941 tcggccctct ggatgtcggc caacaaactt aaagccaatt gtctacacat ggcgattgaa
     3001 cgacgcaaca tcaccatcgc ttgtaagctg gtggaactcc tgcaggacca ccacctcctg
     3061 gacatgctcg atcttttcaa cgaacgcaac gaaaccgccc tgcatttggc cgtttcggcc
     3121 aatctggtag aggtcgtcga cgtcctgctc ctaaccggtt cccggattag ctactgtgac
     3181 agccggggga actccgctct gcatcgggcc gtgtatgaga acgccttgga tagtttgaac
     3241 gtgctgttgg gtcattgcaa acggaacggg ttccggttgg attcgacaaa tgatgatgga
     3301 ttcacggcgc tgcatttggc ggtgatgtgt aaaaatttga aggccacgaa ggttctcctg
     3361 gatcggggcg cttcgtacgt gctgcgtgat cttaagcatg gaaacaacat tcttcatatt
     3421 gccgtggaga gcgattcttt ggacatggtc aattttatcc ttgagggtgt agacaagacg
     3481 ttagctgatg aaccgaacaa tgccggttac acaccacttc agctggctaa cgcaaggaat
     3541 ttggcaaacg cgaacaacaa actaattgtc cgcgaattgc ttcgatataa tccaagcgga
     3601 gtactagaaa aagaacatct caccgaagaa gaagacgacg atcaagaaca ggaagagaac
     3661 cttcccccta gcagcgattc gcagtctgaa accgtgctcg agtcgatgaa tgtgagctgt
     3721 aatagagtgg aggttatacg gttactagaa gactattctc caccgaacga tgaaccaaag
     3781 ttaacaccgc tggagaacgt tccttacaat agcaccctgg aggatggatc cgtctatctg
     3841 tttgatgagg tgtgcttgtc tcacctgtgc ggtcttctga accgaaagaa cctttggcgc
     3901 gaggttgggt cgcttctgga cttcaactca ttcttcatga tctgggaaac gtcggtgaac
     3961 cccgcaggga tgttgctgaa ctactttgag atgcaaaaag tgaaactcga gcacttgatc
     4021 gatgtgctgc aagctttgga ccaaaaagaa gccattcact acatcgacga gatgatttgt
     4081 cggcagatga agtaactcga gcactaagtg attaacctca ggttatacat atattttgaa
     4141 tttaattaat tatacatata ttttatatta tttttgtctt ttattatcga ggggccgttg
     4201 ttggtgtggg gttttgcata gaaataacaa tgggagttgg cgacgttgct gcgccaacac
     4261 cacctccctt ccctcctttc atcatgtatc tgtagataaa ataaaatatt aaacctaaaa
     4321 acaagaccgc gcctatcaac aaaatgatag gcattaactt gccgctgacg ctgtcactaa
     4381 cgttggacga tttgccgact aaaccttcat cgcccagtaa ccaatctaga cgtcaggtgg
     4441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6421 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6481 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6541 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt
     6601 cccggg
//
