LOCUS       sequence-309442-        8858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for insertion of mPlum and the constitutive acpP 
            promoter into the S. Typhimurium chromosome. mPlum is in the forward
            orientation relative to the kanamycin resistance selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Holden ER, Wickham GJ, Webber MA, Thomson NM, Trampari E
  TITLE     Donor plasmids for phenotypically neutral chromosomal gene 
            insertions in Enterobacteriaceae.
  JOURNAL   Microbiology (Reading). 2020 Nov 23. doi: 10.1099/mic.0.000994.
  PUBMED    33226934
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading). 2020 Nov 23. doi: 10.1099/mic.0.000994."
COMMENT     Sequence Label: pDOC-K-glmS-mPlum
FEATURES             Location/Qualifiers
     source          1..8858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1720..1739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1973..1990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2107..2128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2143..2148,2149..2166,2167..2173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2181..2197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2186..2208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2205..2221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2205..2221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2724..2771)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2981..3775
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3035..3054)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3645..3664
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(3966..3999)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             4122..4802
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     4523..4542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4717..4740
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5282..5299)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5282..5298)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5291..5313)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5478..5495)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5648..5734
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5826..5853
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     oriT            6300..6409
                     /note="incP origin of transfer"
     promoter        6783..7228
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7229..7315,7316..8650)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(8689..8708)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8808..8830
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta gccaccactt caagaactct gtagcaccgc ctacatacct
     1441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1861 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1921 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1981 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2041 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2101 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2161 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2221 ccatgattac gccaagctct agggataaca gggtaatcga tgaattcgct cgaaggcgcg
     2281 ctgaagctga aagagatctc ttacattcac gcggaagcct atgctgcagg cgagctgaaa
     2341 cacgggccgt tggcgctgat tgacgccgat atgccggtga ttgtggtggc gccgaacaac
     2401 gaactgctgg agaaactgaa atccaacatc gaagaagttc gcgcacgtgg cggtcagttg
     2461 tacgtcttcg ccgatcagga tgcgggtttc gtcagcaacg acaacatgca catcatcgag
     2521 atgccgcatg tggaagaggt cattgcgcct atcttctaca ccgttccgct gcaactgctg
     2581 gcgtatcacg tggcgctgat taaaggcacc gacgttgacc agccgcgtaa cctggcgaaa
     2641 tcggttaccg tagagtaagt acaaatgtag taccaggcag aaggccgggt acctaggacc
     2701 ggtcaattgg ctggagctgc ttcgaagttc ctatactttc tagagaatag gaacttcgga
     2761 ataggaactt caagatcccc cacgctgccg caagcactca gggcgcaagg gctgctaaag
     2821 gaagcggaac acgtagaact taagggaatt gccagctggg gcgccctctg gtaaggttgg
     2881 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
     2941 atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     3001 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3061 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3121 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3181 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3241 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3301 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3361 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3421 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3481 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     3541 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3601 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3661 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgctct acggtatcgc
     3721 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     3781 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     3841 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     3901 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccag cttcaaaagc
     3961 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac taaggaggat
     4021 attcatatct cgagcatatg cccgttgcaa atttttcaac atttatacac tacgaaaacc
     4081 atcgcgaaag cgagttttgg atttaagaag gagatataca tatggtgagc aagggcgagg
     4141 aggtcatcaa ggagttcatg cgcttcaagg agcacatgga gggctccgtg aacggccacg
     4201 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaggc
     4261 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagatca
     4321 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     4381 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     4441 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aaggtgcgcg
     4501 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4561 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatgaag atgaggctga
     4621 ggctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc
     4681 ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg
     4741 aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgcct
     4801 aaggggcggc cgcaagcttt cgacagacgg cctttttttg tgcgctgtga caggcgctgt
     4861 tctttctcta tggtggctga agagcgacta agaggtgcta cgcctgcctg cgctacgggc
     4921 acccggtcac attctcttct ttaaggccct ggctcaatcc aggtggatcg gaaatcaaac
     4981 caccccaggt tattcaggtg gatccccctg gcccggtcgg ggcttttgag tcgcatccag
     5041 tggtgaaaaa gcggctgtag ccagcctgaa cgggtggttt ccctgaccag ttgttccgcg
     5101 ctgatggttt cggcatgcca ctgggtttcc cattgggcca gcagcgcatc atccccacca
     5161 ctgatggcgt ggcgcagtaa aggggagccc agtagccatg taccgacgtt ccactcttcg
     5221 ggaatgggga aattgaccgg ctagcgtcga ctagtaggga taacagggta atgagcttgg
     5281 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5341 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5401 gcccttccca acagttgcgc agcctgaatg gcgaatggcg agcttggctg ttttggcgga
     5461 tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     5521 cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     5581 gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
     5641 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     5701 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     5761 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     5821 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     5881 ttttctaaat acattcaaat atgcatgcgc ctgatgcggt attttctcct tacgcatctg
     5941 tgcggtattt cacaccgcat atcgacatcc gccctcaccg ccaggaacgc aaccgcagcc
     6001 tcatcacgcc ggcgcttctt ggccgcgcgg gattcaaccc actcggccag ctcgtcggtg
     6061 tagctctttg gcatcgtctc tcgcctgtcc cctcagttca gtaatttcct gcatttgcct
     6121 gtttccagtc ggtagatatt ccacaaaaca gcagggaagc agcgcttttc cgctgcataa
     6181 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     6241 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     6301 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     6361 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
     6421 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     6481 cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg
     6541 gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc
     6601 gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg
     6661 ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc
     6721 acgatcctcg ccctgctggc gaagatcgac tctagctaga ggatcgatcc tttttaaccc
     6781 atcacatata cctgccgttc actattattt agtgaaatga gatattatga tattttctga
     6841 attgtgatta aaaaggcaac tttatgccca tgcaacagaa actataaaaa atacagagaa
     6901 tgaaaagaaa cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg
     6961 attttctatc aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag
     7021 aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa
     7081 tgtgtaaagg gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt
     7141 attgtgcgta actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac
     7201 acagtacata aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac
     7261 agtattaacc tttactaccg cactgctggc aggaggcgca actcaagcgt ttgcgaaaga
     7321 aacgaaccaa aagccatata aggaaacata cggcatttcc catattacac gccatgatat
     7381 gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa gttcctgagt tcgattcgtc
     7441 cacaattaaa aatatctctt ctgcaaaagg cctggacgtt tgggacagct ggccattaca
     7501 aaacgctgac ggcactgtcg caaactatca cggctaccac atcgtctttg cattagccgg
     7561 agatcctaaa aatgcggatg acacatcgat ttacatgttc tatcaaaaag tcggcgaaac
     7621 ttctattgac agctggaaaa acgctggccg cgtctttaaa gacagcgaca aattcgatgc
     7681 aaatgattct atcctaaaag accaaacaca agaatggtca ggttcagcca catttacatc
     7741 tgacggaaaa atccgtttat tctacactga tttctccggt aaacattacg gcaaacaaac
     7801 actgacaact gcacaagtta acgtatcagc atcagacagc tctttgaaca tcaacggtgt
     7861 agaggattat aaatcaatct ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt
     7921 catcgatgaa ggcaactaca gctcaggcga caaccatacg ctgagagatc ctcactacgt
     7981 agaagataaa ggccacaaat acttagtatt tgaagcaaac actggaactg aagatggcta
     8041 ccaaggcgaa gaatctttat ttaacaaagc atactatggc aaaagcacat cattcttccg
     8101 tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg
     8161 cgctctcggt atgattgagc taaacgatga ttacacactg aaaaaagtga tgaaaccgct
     8221 gattgcatct aacacagtaa cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg
     8281 caaatggtat ctgttcactg actcccgcgg atcaaaaatg acgattgacg gcattacgtc
     8341 taacgatatt tacatgcttg gttatgtttc taattcttta actggcccat acaagccgct
     8401 gaacaaaact ggccttgtgt taaaaatgga tcttgatcct aacgatgtaa cctttactta
     8461 ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc gtgattacaa gctatatgac
     8521 aaacagagga ttctacgcag acaaacaatc aacgtttgcg cctagcttcc tgctgaacat
     8581 caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt gaacaaggac aattaacagt
     8641 taacaaataa aaacgcaaaa gaaaatgccg attatggtgc actctcagta caatctgctc
     8701 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     8761 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     8821 gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
