LOCUS       sequence-309443-       15112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level M binary vector for plant transformation; for cloning Cas9 and
            guide RNAs; single copy in Agrobacterium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT,
            Stuttmann J, Marillonnet S
  TITLE     High-efficiency genome editing in plants mediated by a Cas9 gene 
            containing multiple introns.
  JOURNAL   Plant Commun. 2020 Nov 23;2(2):100135. doi: 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8.
  PUBMED    33898975
REFERENCE   2  (bases 1 to 15112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2020-11-23"; volume: "2(2):100135. doi"; pages: " 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8"
COMMENT     Sequence Label: pAGM41991
FEATURES             Location/Qualifiers
     source          1..15112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..50
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5052..5843
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6184..6772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6673..6692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6908..6932
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      13124..13133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
       61 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
      121 catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgccgcaag ggtgacttcg
      181 cctatattgg acgacggcgc gcagagggcg acctcttttt gggttacgat tgtaggatta
      241 tcactaaaac aatacatgaa catattcaaa tggcaatctc tctaaggcat tggaaataaa
      301 tacaaataac agttgggtgg agtttttcga cctgagggcg ttaaccttct gttaacctaa
      361 aagctcttgc ccaaacagca gaatcggcgc taattgccag cggcggaact tttccagttt
      421 cgcgaaaaat atcgccactg gcaaggaatg ggtttgagat ggcgaagtct gtcctaaaag
      481 cagcgcctgt agttgtaggg ttgacggcct tgatggagcg tcatgccgat gccctctcga
      541 gccaacttca agcacatcat cttaaggttt tcccgccgca ttccgagaag ggcattcgaa
      601 cattcgggcc atcggaggcg tccaagctgc tcggcgttgg cgagtcatat ttacggcaga
      661 ccgcgtctga gatgccagag ttgaatgtta gcatgagccc gggtggcagg cgaatgttct
      721 caattgaaga tatccatgtg attcggaagt atatggatca ggtcggccgc gggaaccggc
      781 gctacctgcc acatcgtcga ggcggcgagc agcttcaggt tatctctgtg atgaatttca
      841 aaggtgggtc gggtaagacc accaccgccg cgcatctggc gcagtacctc gctatgcgcg
      901 gatatcgagt cttggccatt gatctcgatc ctcaagcgag cctttctgca ctctttggga
      961 gccaaccgga aacggacgtt ggcccgaacg aaacgctcta cggcgctata aggtatgatg
     1021 atgagcaggt ggcaatcgaa cgagtcgtcc gagggactta cattcccgac ctccacctga
     1081 ttcctggtaa ccttgagctg atggagtttg aacacgatac gccacgcgcg ctgatgaacc
     1141 gcaaagaggg cgacacgctc ttttatggtc gcatcagcca agtaattgaa gatatcgcgg
     1201 ataactatga cgtcgtggtc atcgactgcc ctccccagct tgggtatctc acgctatccg
     1261 cattgactgc ggcgacgtcc attcttgtca cggtccatcc gcagatgctg gatgtgatgt
     1321 cgatgaacca gtttctggca atgacatcga accttttgcg tgaaatcgag aatgctggcg
     1381 ccaagttcaa gtttaattgg atgcgctatc tgataacccg tttcgaaccg agcgacggac
     1441 cacagaacca aatggtaggt tatctgcggt cgatttttgg cgaaaatgtc ctcaattttc
     1501 cgatgcttaa aaccaccgcg gtttcggacg ctggcctgac aaaccagact ctattcgaag
     1561 tggagcgtgg cctgttcacg cgctcgacct atgatcgagc cttggaggcg atgaacgccg
     1621 tcaacgacga gatcgaaaca ctgatcaaaa aagcatgggg taggcccaca tgagccggaa
     1681 gcacatcctt ggcgtgtcaa ctgacgcccc tgaaacgtcg cccgccgaca ataggacggc
     1741 aaagaaccgc tccatgccgc tcctcggcgt aacaaggaag gagcgcgatc cggcaacgaa
     1801 gctcacagcg aacattggta acgcactgcg agagcaaaac gatcgtctta gccgtgccga
     1861 agagatcgag cggcgactcg ctgaaggtca ggcagtgata gagttggatg cctcgtcaat
     1921 agaaccgtct ttcgtgcagg atcgtatgcg aggggacatt gacgggctcc ttacttcgat
     1981 ccgggaacaa ggacagcaag tcccaatcct tgtgcgaccg catccgagcc agccgggccg
     2041 atatcaggtt gccttcggcc accgccggct acgcgccgtt tcagaactcg gacttccggt
     2101 cagagcggtc gttcgcgaac tgacggacga gcaagtggtc gtagcacagg gtcaggaaaa
     2161 caatgagcgc gaagatctta ccttcatcga aaaggcgcgc ttcgcacatc gcctgaacag
     2221 gcagttttct cgagagattg tcatcgccgc gatgtcgatc gacaagagca atttgtccaa
     2281 gatgcttctg ctcgtcgacg ccctcccctc tgaactgacc gatgctattg gtgccgctcc
     2341 tggtgttgga cggccgagtt ggcaacaact tgccgagctg attgagaaag tttcttcacc
     2401 ggccgacgtg gctaaatatg ctatgtcgga ggaagttcaa gcgctgccat cggcagaacg
     2461 attcaaggcg gtgatcgcta gtctgaagcc cagtcgggtt gcgcgtggac ttcccgaggt
     2521 catggccacc ccagacggca ccagaattgc acaggtgacg cagagcaagg ccaaactgga
     2581 aatcacgatt gacaggaagg cgacgcccga ttttgcgacc ttcgtgctcg atcatgtgcc
     2641 agcgctgtat caagcgtacc acgctgagaa ccaacggaaa cggggagagt aaaccgcaaa
     2701 agaaaagagc cccctcaacg tcgccgtcgc ggaagccctt ctgtctctct agcgcgaaca
     2761 gaatcgcatt tcctcgaatc ctcgtcaaga gtttttagcg ccgttttggt gagctgattt
     2821 cctttgcctg ctgaaaggtg aaagatgatg cagacaggaa gtgtaacgac gccattcggg
     2881 cggcggccaa tgacgcttgc gcttgtgcgg cgccagacgg cgctggccga tatcaaacaa
     2941 ggcaagacag cggacaagtg gaaggtcttt agagatgcgt ccgcggccat ggaactactt
     3001 ggaatccagt ccaacagtct tgccgtcctt gatgcgctat tgagctttca cccggaaacg
     3061 gagttgcgtc aggaggcaca gctgatcgtg ttcccgtcga atgctcagct tgcccttcgg
     3121 gcgcatggga tggctggcgc gactttgcgt aggcacatcg ccatgctcgt ggagtcaggc
     3181 ttgatcgtcc ggaaggatag cgccaacgga aagcgttacg ctcgtaagga tggcgctggt
     3241 cagatcgagc gcgcgtttgg cttcgatttg tctccgcttc tcgcgcggtc cgaggagcta
     3301 gcgatgatgg cacagcaggt gatggccgat cgagcagcat tcaggatggc caaagaaagt
     3361 ctgacgattt gccgacggga cgttcggaag ctaattacgg cagctatgga agagggagcg
     3421 gagggcgact ggcaagctgt cgaggaagtc tatgtggaac ttgtgggtag aattccacgc
     3481 gccccgacgc ttgctgatgt agagtcaatt ctcgaagaga tgtggatgct ccaggaagag
     3541 ataatcaacc ggttggaaat tagagacaat tcagaaaata atagcaccaa tgctgcccag
     3601 agcgagcagc acatacagaa ttcaaaaccc gaatccgtta atgaacttga acctcgctct
     3661 gaaaaggagc agggcgctaa gccgagtgaa atagaccggg caaggagcga gccgataaaa
     3721 gcgttccccc tcgggatgat cctgaaagca tgcccgacca ttggcaatta tgggccgagc
     3781 ggtgcggttg ctagctggcg tgacctcatg tcggctgcgg tggtggttcg gtctatgctg
     3841 ggggtcagcc cgtcggctta ccaagacgcg tgtgaggcaa tgggaccgga gaatgcggca
     3901 gcagcgatgg cgtgcatttt ggagcgagcg aacttcatca attcgcccgg gggctatctc
     3961 cgagatctga cacggcggag cgagctcggg aagttttcac ttggcccgat gataatggcg
     4021 ctcttgaagg ctagcgggca ggggacgttg cggtttggct agaattagcg agtatggagc
     4081 aggatggtct gtggtcagct gaccacagac ctaataggtt gaaaacatga gcgttttttg
     4141 gatgatcgac agaccatccg attcccggag taccaagcgt gctctgatgg gagcgataac
     4201 attactcaac aagcacgaag gccccatgcc gatcgttgat cgtgaaggag agcctgctct
     4261 acatgcggcg gtattttgcc ggccgaggca tgtagtcgcg gagcactgcc tatttactgc
     4321 cctaggcaca aacgttgact cttggatcga gctggcagac aaagcaataa cccacacaga
     4381 ggacgattaa tggctgacga agagatccag aatccgccgg acggtactgc tgctgccgaa
     4441 gttgagccgg ctgctcctag aggtagaaga gaaaagaaag caccagccga aacagcccgc
     4501 acgggatcgt tcaaatccgt gaagccgaaa acccgcggcc tcagcaaccg agaaaaactg
     4561 gagaagatcg gtcaaatcga agctcaggtc gctggcggcg caaccttgaa ggacgccgtt
     4621 aagatcgtgg gtatttccgt tcagacctat tatcaatgga agagagctgc ggttcaacct
     4681 gtctcacaga atccggccgt gtctgtttca gttgacgatg aactcggcga gttcatccaa
     4741 ctcgaggagg aaaatcggcg gctcagaaag ccgaggcacg aacccagtgg acataagcct
     4801 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     4861 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     4921 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     4981 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     5041 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     5101 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     5161 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
     5221 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     5281 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     5341 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     5401 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     5461 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     5521 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     5581 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     5641 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     5701 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     5761 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgtg aaaggcgaga
     5821 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc
     5881 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     5941 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     6001 gagatatatc atgctgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6061 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6781 caacgcggcc tttttacggt tcctgctcgg atctgttgga ccggacagta gtcatggttg
     6841 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     6901 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     6961 cattgcggac gtttttaatg tactggggtt gaacactctg tggtctcatg ccttgtcttc
     7021 cagtggtatg gggtaccgca cggtctgcca atccgacgga ggtttatgaa ttttccacct
     7081 tttccacaag ctcaactagt attaacgatg tggatttagc aaaaaaaacc tgtaacccta
     7141 aatgtaaaat aacgggtaag cctgccaacc atgttatggc agattaagcg tctttttgaa
     7201 gggcaccgca tctttcgcgt tgccgtaaat gtatccgttt agcaataagg aaattcttaa
     7261 tgaataatcc gtcgttactc aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt
     7321 ttgcgacagc ctcaaagtta tttgatgcaa aaacccggcg cagcgtactg atgctctacg
     7381 cctggtgccg ccattgtgac gatgttattg acgatcagac gctgggcttt caggcccggc
     7441 agcctgcctt acaaacgccc gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg
     7501 cctatgcagg atcgcagatg cacgaaccgg cgtttgcggc ttttcgggaa gtggctatgg
     7561 ctcatgatat cgccccggct tacgcgtttg atcatctgga aggcttcgcc atggatgtac
     7621 gcgaagcgca atacagccaa ctggatgata cgctgcgcta ttgctatcac gttgcaggcg
     7681 ttgtcggctt gatgatggcg caaatcatgg gcgtgcggga taacgccacg ctggaccgcg
     7741 cctgtgacct tgggctggca tttcagttga ccaatattgc tcgcgatatt gtggacgatg
     7801 cgcatgcggg ccgctgttat ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag
     7861 agaattatgc ggcacctgaa aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc
     7921 aggaagcaga accttactat ttgtctgcca cagccggcct ggcagggttg cccctgcgtt
     7981 ccgcctgggc aatcgctacg gcgaagcagg tttaccggaa aataggtgtc aaagttgaac
     8041 aggccggtca gcaagcctgg gatcagcggc agtcaacgac cacgcccgaa aaattaacgc
     8101 tgctgctggc cgcctctggt caggccctta cttcccggat gcgggctcat cctccccgcc
     8161 ctgcgcatct ctggcagcgc ccgctctagg cttactataa ggatgtttgc aatgaaacca
     8221 actacggtaa ttggtgcagg cttcggtggc ctggcactgg caattcgtct acaagctgcg
     8281 gggatccccg tcttactgct tgaacaacgt gataaacccg gcggtcgggc ttatgtctac
     8341 gaggatcagg ggtttacctt tgatgcaggc ccgacggtta tcaccgatcc cagtgccatt
     8401 gaagaactgt ttgcactggc aggaaaacag ttaaaagagt atgtcgaact gctgccggtt
     8461 acgccgtttt accgcctgtg ttgggagtca gggaaggtct ttaattacga taacgatcaa
     8521 acccggctcg aagcgcagat tcagcagttt aatccccgcg atgtcgaagg ttatcgtcag
     8581 tttctggact attcacgcgc ggtgtttaaa gaaggctatc taaagctcgg tactgtccct
     8641 tttttatcgt tcagagacat gcttcgcgcc gcacctcaac tggcgaaact gcaggcatgg
     8701 agaagcgttt acagtaaggt tgccagttac atcgaagatg aacatctgcg ccaggcgttt
     8761 tctttccact cgctgttggt gggcggcaat cccttcgcca cctcatccat ttatacgttg
     8821 atacacgcgc tggagcgtga gtggggcgtc tggtttccgc gtggcggcac cggcgcatta
     8881 gttcagggga tgataaagct gtttcaggat ctgggtggcg aagtcgtgtt aaacgccaga
     8941 gtcagccata tggaaacgac aggaaacaag attgaagccg tgcatttaga ggacggtcgc
     9001 aggttcctga cgcaagccgt cgcgtcaaat gcagatgtgg ttcataccta tcgcgacctg
     9061 ttaagccagc accctgccgc ggttaagcag tccaacaaac tgcagactaa gcgcatgagt
     9121 aactctctgt ttgtgctcta ttttggtttg aatcaccatc atgatcagct cgcgcatcac
     9181 acggtttgtt tcggcccgcg ttaccgcgag ctgattgacg aaatttttaa tcatgatggc
     9241 ctcgcagagg acttctcact ttatctgcac gcgccctgtg tcacggattc gtcactggcg
     9301 cctgaaggtt gcggcagtta ctatgtgttg gcgccggtgc cgcatttagg caccgcgaac
     9361 ctcgactgga cggttgaggg gccaaaacta cgcgaccgta tttttgcgta ccttgagcag
     9421 cattacatgc ctggcttacg gagtcagctg gtcacgcacc ggatgtttac gccgtttgat
     9481 tttcgcgacc agcttaatgc ctatcatggc tcagcctttt ctgtggagcc cgttcttacc
     9541 cagagcgcct ggtttcggcc gcataaccgc gataaaacca ttactaatct ctacctggtc
     9601 ggcgcaggca cgcatcccgg cgcaggcatt cctggcgtca tcggctcggc aaaagcgaca
     9661 gcaggtttga tgctggagga tctgatatga gcttttaccc aggagacaga gaatgcaacc
     9721 gcattatgat ctgattctcg tgggggctgg actcgcgaat ggccttatcg ccctgcgact
     9781 tcagcagcag caacctgata tgcgtatttt gcttatcgac gccgcacccc aggcgggcgg
     9841 gaatcatacg tggtcatttc accacgatga tttgactgag agccaacatc gttggatagc
     9901 tccgctggtg gttcatcact ggcccgacta tcaggtacgc tttcccacac gccgtcgtaa
     9961 gctgaacagc ggctactttt gtattacttc tcagcgtttc gctgaggttt tacagcgaca
    10021 gtttggcccg cacttgtgga tggataccgc ggtcgcagag gttaatgcgg aatctgttcg
    10081 gttgaaaaag ggtcaggtta tcggtgcccg cgcggtgatt gacgggcggg gttatgcggc
    10141 aaattcagca ctgagcgtgg gcttccaggc gtttattggc caggaatggc gattgagtca
    10201 cccgcatggt ttatcatctc cgattatcat ggatgccacg gtcgatcagc aaaatggtta
    10261 tcgcttcgtg tacagcctgc cgctctcgcc gaccagattg ttaattgaag atacgcacta
    10321 tattgataat gcgacattag atcctgaatg cgcgcggcaa aatatttgcg actatgccgc
    10381 gcaacagggt tggcagcttc agacactgct gcgagaagaa cagggcgcct tacccattac
    10441 tctgtcgggc aatgccgacg cattctggca gcagcgcccc ctggcctgta gtggattacg
    10501 tgccggtctg ttccatccta ccaccggcta ttcactgccg ctggcggttg ccgtggccga
    10561 ccgcctgagt gcacttgatg tctttacgtc ggcctcaatt caccatgcca ttacgcattt
    10621 tgcccgcgag cgctggcagc agcagggctt tttccgcatg ctgaatcgca tgctgttttt
    10681 agccggaccc gccgattcac gctggcgggt tatgcagcgt ttttatggtt tacctgaaga
    10741 tttaattgcc cgtttttatg cgggaaaact cacgctgacc gatcggctac gtattctgag
    10801 cggcaagccg cctgttccgg tattagcagc attgcaagcc attatgacga ctcatcgtta
    10861 agcttcagag gaggaattaa gtaatgagtt ttgatattgc caaatacccg accctggcac
    10921 tggtcgactc cacccaggag ttacgactgt tgccgaaaga gagtttaccg aaactctgcg
    10981 acgaactgcg ccgctattta ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg
    11041 ggctgggcac ggtcgaactg accgtggcgc tgcactatgt ctacaacacc ccgtttgacc
    11101 aattgatttg ggatgtgggg catcaggctt atccgcataa aattttgacc ggacgccgcg
    11161 acaaaatcgg caccatccgt cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa
    11221 gcgaatatga cgtattaagc gtcgggcatt catcaacctc cattagtgcc ggaattggta
    11281 ttgcggttgc tgccgaaaaa gaaggcaaaa atcgccgcac cgtctgtgtc attggcgatg
    11341 gcgcgattac cgcaggcatg gcgtttgaag cgatgaatca cgcgggcgat atccgtcctg
    11401 atatgctggt gattctcaac gacaatgaaa tgtcgatttc cgaaaatgtc ggcgcgctca
    11461 acaaccatct ggcacagctg ctttccggta agctttactc ttcactgcgc gaaggcggga
    11521 aaaaagtttt ctctggcgtg ccgccaatta aagagctgct caaacgcacc gaagaacata
    11581 ttaaaggcat ggtagtgcct ggcacgttgt ttgaggagct gggctttaac tacatcggcc
    11641 cggtggacgg tcatgatgtg ctggggctta tcaccacgct aaagaacatg cgcgacctga
    11701 aaggcccgca gttcctgcat atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa
    11761 aagacccgat cactttccat gccgtgctaa atttgatccc tccagcggtt gtttgccgaa
    11821 aagtagcggc ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc
    11881 agcgaaagac aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt
    11941 cgagttttca cgtaaattcc cggatcgcta cttcgacgtg gcaattgccg agcaacatgc
    12001 ggtgaccttt gctgcgggtc tggcgattgg tgggtacaaa cccattgtcg cgatttactc
    12061 cactttcctg caacgcgcct atgatcaggt gctgcatgac gtggcgattc aaaagcttcc
    12121 ggtcctgttc gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg
    12181 tgcttttgat ctctcttacc tgcgctgcat accggaaatg gtcattatga ccccgagcga
    12241 tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg gcccgtcagc
    12301 ggtgcgctac ccgcgtggca acgcggtcgg cgtggaactg acgccgctgg aaaaactacc
    12361 aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta actttggtac
    12421 gctgatgcca gaagcggcga aagtcgccga atcgctgaac gccacgctgg tcgatatgcg
    12481 ttttgtgaaa ccgcttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct
    12541 ggtcaccgta gaagaaaacg ccattatggg cggcgcaggc agcggcgtga acgaagtgct
    12601 gatggcccat cgtaaaccag tacccgtgct gaacattggc ctgccggact tctttattcc
    12661 gcaaggaact caggaagaaa tgcgcgccga actcggcctc gatgccgctg gtatggaagc
    12721 caaaatcaag gcctggctgg cataagcttt gtgtgaggag atgtcgaatg agcgcacatg
    12781 ccctgcccaa ggcagatctg accgccacca gcctgatcgt atcgggcggc atcatcgccg
    12841 cttggctggc cctgcatgtg catgcgctgt ggtttctgga cgcagcggcg catcccatcc
    12901 tggcgatcgc aaatttcctg gggctgacct ggctgtcggt cggattgttc atcatcgcgc
    12961 atgacgcgat gcacgggtcg gtggtgccgg ggcgtccgcg cgccaatgcg gcgatgggcc
    13021 agcttgtcct gtggctgtat gccggatttt cgtggcgcaa gatgatcgtc aagcacatgg
    13081 cccatcaccg ccatgccgga accgacgacg accccgattt cgaccatggc ggcccggtcc
    13141 gctggtacgc ccgcttcatc ggcacctatt tcggctggcg cgaggggctg cttctgcccg
    13201 tcatcgtgac ggtctatgcg ctgatccttg gggatcgctg gatgtacgtg gtattctggc
    13261 cgctgccgtc gatcctggcg tcgatccagc tgttcgtgtt cggcacctgg ctgccgcacc
    13321 gccccggcca cgacgcgttc ccggaccgcc acaatgcgcg gtcgtcgcgg atcagcgacc
    13381 ccgtgtcgct gctgacctgt tttcactttg gcggttatca tcacgaacac cacctgcacc
    13441 cgacggtgcc gtggtggcgc ctgcccagca cccgcaccaa gggcgacacc gcataagctt
    13501 gagctgagga gattttgaat gcaaacggaa cacgtcattt tattgaatgc acagggagtt
    13561 cccacgggta cgctggaaaa gtatgccgca cacacggcag acacccgctt acatctcgcg
    13621 ttctccagtt ggctgtttaa tgccaaagga caattattag ttacccgccg cgcactgagc
    13681 aaaaaagcat ggcctggcgt gtggactaac tcggtttgtg ggcacccaca actgggagaa
    13741 agcaacgagg acgcagtgat ccgccgttgc cgttatgagc ttggcgtgga aattacgcct
    13801 cctgaatcta tctatcctga ctttcgctac cgcgccaccg atccgagtgg cattgtggaa
    13861 aatgaagtgt gtccggtatt tgccgcacgc accactagtg cgttacagat caatgatgat
    13921 gaagtgatgg attatcaatg gtgtgattta gcagatgtat tacacggtat tgatgcaacg
    13981 ccgtgggcgt tcagtccgtg gatggtgatg caggcgacaa atcgcgaagc cagaaaacga
    14041 ttatctgcat ttacccagct taaataagct ttgcctcagg atttttgaaa tgacggtctg
    14101 cgcaaaaaaa cacgttcatc tcactcgcga tgctgcggag cagttactgg ctgatattga
    14161 tcgacgcctt gatcagttat tgcccgtgga gggagaacgg gatgttgtgg gtgccgcgat
    14221 gcgtgaaggt gcgctggcac cgggaaaacg tattcgcccc atgttgctgt tgctgaccgc
    14281 ccgcgatctg ggttgcgctg tcagccatga cggattactg gatttggcct gtgcggtgga
    14341 aatggtccac gcggcttcgc tgatccttga cgatatgccc tgcatggacg atgcgaagct
    14401 gcggcgcgga cgccctacca ttcattctca ttacggagag catgtggcaa tactggcggc
    14461 ggttgccttg ctgagtaaag cctttggcgt aattgccgat gcagatggcc tcacgccgct
    14521 ggcaaaaaat cgggcggttt ctgaactgtc aaacgccatc ggcatgcaag gattggttca
    14581 gggtcagttc aaggatctgt ctgaagggga taagccgcgc agcgctgaag ctattttgat
    14641 gacgaatcac tttaaaacca gcacgctgtt ttgtgcctcg atgcagatgg cctcgattgt
    14701 tgcgaatgcc tccagcgaag cgcgtgattg cctgcatcgt ttttcacttg atcttggtca
    14761 ggcatttcaa ctgctggacg atttgaccga tggcatgacc gacaccggta aggatagcaa
    14821 tcaggacgcc ggtaaatcga cgctggtcaa tctgttaggc ccgagggcgg ttgaagaacg
    14881 tctgagacaa catcttcagc ttgccagtga gcatctctct gcggcctgcc aacacgggca
    14941 cgccactcaa cattttattc aggcctggtt tgacaaaaaa ctcgctgccg tcagttaagc
    15001 ttgcaagaat tcaagcttgg agtacgttaa ttagttgtct gttttaagcc ccgtgctctc
    15061 cgccggggct tttttattaa aaaccactca agtggaagac aagggacacg aa
//
