LOCUS       sequence-309445-        8665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible mammalian expression plasmid for halo tag fusion 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Huen Lab
  TITLE     Gateway compatible mammalian expression plasmid for HaloTag fusion 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMH-Halo tag
FEATURES             Location/Qualifiers
     source          1..8665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..233
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(242..670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(352..373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     564..583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    716..940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     929..946
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        966..984
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     966..983
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1012..1136
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1177..1482)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1279..1298)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1802..2482)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     2397..2416
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2536..2542,2543..2560,2561..2566))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2591..2715)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2722..3612)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      3609..3618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3625..3654)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3654..3663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          3712..4284
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4320..4339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4321..4339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4384..4587)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4413..4433)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4588..4967
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5139..5158
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5233..5337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5338..5406,5407..6198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5556..5575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6369..6957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6858..6877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7111..7128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7245..7266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7281..7286,7287..7304,7305..7311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7319..7335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7324..7346
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7343..7359
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7343..7359
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7408..7529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7419..7438)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7473..7492
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7703..8497)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7814..7833)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8424..8443
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(8564..8665,1..228))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8578..8665,1..48)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8632..8651)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
       61 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      121 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
      181 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat
      241 taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      361 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      421 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      481 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      541 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
      601 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      661 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
      721 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
      781 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
      841 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
      901 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgagct
      961 ctagcattta ggtgacacta tagaataggg ccctctagat gcatgctcga gaccactttg
     1021 tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     1081 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggtcg
     1141 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
     1201 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     1261 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     1321 caccaccggg taaagttcac gggggacttt atctgacagc agacgtgcac tggccagggg
     1381 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     1441 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
     1501 cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
     1561 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     1621 ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     1681 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata
     1741 tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct
     1801 tttagtaagc cggatcctct agattacgcc ccgcctgcca ctcatcgcag tactgttgta
     1861 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1921 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1981 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     2041 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     2101 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2161 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2221 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     2281 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     2341 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2401 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2461 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct
     2521 aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2581 atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2641 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2701 tttgtacaaa cttgtaccgg tgccggaaat ttctagcgtc gacagccagc gcgcgatctc
     2761 gctgccgatc aggtccgggt tgtcttcttg cagcagattc agacccgggc cgatgtccac
     2821 agccttgcag ttaggcaggc ttttggccag gcgagcggct tcggccggtg ggatcagaac
     2881 gcctggggtg ccccagaaca gcagcttcgg gacaggggac tggtgcagcc agtccatgta
     2941 ttcttcgacc agcgcgacga tgttcgctgg ctcaccggcg attggcagct cgtttgggaa
     3001 gcgccacagt ggctcgcggt caacaggatt caggaacggc tcgcggtaat ggtccatctc
     3061 gacttcagtc agcgggcgga cgacacccat cggcagcgta ccctcgataa aaacgttctg
     3121 atcgatgatc agcttgcggc cgacgtcggt ggtgcggaag gcctggaagg tctcgcgggc
     3181 aaattctggc cattcgtccc aggtcgggat agggcggatg aactccataa atgcaatacc
     3241 tttgacgcgc tctggattgc gcttggccca gtggaaaccc agagcggagc cccagtcgtg
     3301 aatgaccagg acgacctctt ccagacccag ggcttcgatg aaggcatcca tgaagcggac
     3361 gtggtcgtcg aagaaataac ccaggtctgg tttgtcggat ttgcccatac cgatcaggtc
     3421 tggagcaatg cagcgatggg tcggtgcaac atgcgggatg atgttgcgcc acacgtagga
     3481 ggaggtcggg ttaccgtgca ggaacagcac aggggtgcca tcgcgcggac caacatcgac
     3541 gtagtgcatg cgctcgccca ggacttccac ataatggggg tcgaatggaa agccagtacc
     3601 gatttctgcc atggtggcgc tagccaagtc ttcttcagaa atcagctttt gttccatggt
     3661 ggctcgcgaa agcttggggt cgacagcaca aaccagcacg ttgcccagga gctgtaggaa
     3721 aaagaagaag gcatgaacat ggttagcaga ggggcccggt ttggactcag agtattttat
     3781 cctcatctca aacagtgtat atcattgtaa ccataaagag aaaggcagga tgatgaccag
     3841 gatgtagttg tttctaccaa taagaatatt tccacgccag ccagaattta tatgcagaaa
     3901 tattctacct tatcatttaa ttataacaat tgttctctaa aactgtgctg aagtacaata
     3961 taatataccc tgattgcctt gaaaaaaaag tgattagaga aagtacttac aatctgacaa
     4021 ataaacaaaa gtgaatttaa aaattcgtta caaatgcaag ctaaagttta acgaaaaagt
     4081 tacagaaaat gaaaagaaaa taagaggaga caatggttgt caacagagta gaaagtgaaa
     4141 gaaacaaaat tatcatgagg gtccatggtg atacaaggga catcttccca ttctaaacaa
     4201 caccctgaaa actttgcccc ctccatataa catgaatttt acaatagcga aaaagaaaga
     4261 acaatcaagg gtccccaaac tcaccctgaa gttctcagga tcgactctag cttgggtctc
     4321 cctatagtga gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca
     4381 gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     4441 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     4501 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     4561 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     4621 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     4681 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     4741 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     4801 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     4861 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     4921 tgaccccgta attgattact attaataact agtcaataat caatgtcaac gcgtatatct
     4981 ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt
     5041 gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc
     5101 caacacacaa gcagggagca gatactggct taactatgcg gcatcagagc agattgtact
     5161 gagagtgcac cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt
     5221 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5341 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5461 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5521 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5581 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5641 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5821 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5881 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5941 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6001 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6061 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6181 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6361 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6421 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6481 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6541 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6601 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6661 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6721 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7141 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7201 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7261 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7321 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     7381 ctagaggtcg acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa
     7441 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     7501 taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc
     7561 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     7621 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     7681 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     7741 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     7801 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     7861 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     7921 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
     7981 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     8041 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     8101 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     8161 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     8221 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     8281 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
     8341 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     8401 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     8461 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     8521 cttgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc
     8581 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct
     8641 ctgcataaat aaaaaaaatt agtca
//
