LOCUS       sequence-309458-       12486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Human TMPRSS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12486)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 12486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Puro-Ak-TMPRSS2
FEATURES             Location/Qualifiers
     source          1..12486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             86..719
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    766..891
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1384..1617
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1802..1846
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2229..3407
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2460..3398
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3355..3375
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3455..3572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5158..5725
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5176..5727
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5320..5337)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5547..5566
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5730..6329
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5730..5749)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6226..6246
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6371..6387)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6372..6388)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6401..6989
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6454..6474)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6872..6883)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7133..7166)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7186..7366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7391..7409)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7392..7409)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7699..7718)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7932..7951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8051..8073
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8111..8129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8197..8301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8302..8370,8371..9162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8520..8539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9333..9921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9822..9841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10075..10092
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10162..10182)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10167..10496
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10347..10482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11630..11695
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11825..11845
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12270..12404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12307..12326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12361..12380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
       61 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
      121 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
      181 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
      241 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
      301 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
      361 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
      421 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
      481 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
      541 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      601 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      661 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      721 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      781 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      841 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      901 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
      961 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1021 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1081 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1141 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1201 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1261 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1321 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1381 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1441 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1501 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1561 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1621 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1681 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1741 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1801 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1861 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1921 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1981 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2041 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2101 gtatcgatca cgagactagc ctcgaccatc gatgtcgacg ataagctttg caaagatgga
     2161 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     2221 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2281 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2341 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2401 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2461 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2521 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     2581 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     2641 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     2701 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     2761 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     2821 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     2881 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     2941 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     3001 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3061 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3121 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3181 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3241 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3301 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     3361 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgacat
     3421 ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     3481 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     3541 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt
     3601 aaacccggga gcaggcacat ggctttgaac tcagggtcac caccagctat tggaccttac
     3661 tatgaaaacc atggatacca accggaaaac ccctatcccg cacagcccac tgtggtcccc
     3721 actgtctacg aggtgcatcc ggctcagtac tacccgtccc ccgtgcccca gtacgccccg
     3781 agggtcctga cgcaggcttc caaccccgtc gtctgcacgc agcccaaatc cccatccggg
     3841 acagtgtgca cctcaaagac taagaaagca ctgtgcatca ccttgaccct ggggaccttc
     3901 ctcgtgggag ctgcgctggc cgctggccta ctctggaagt tcatgggcag caagtgctcc
     3961 aactctggga tagagtgcga ctcctcaggt acctgcatca acccctctaa ctggtgtgat
     4021 ggcgtgtcac actgccccgg cggggaggac gagaatcggt gtgttcgcct ctacggacca
     4081 aacttcatcc ttcaggtgta ctcatctcag aggaagtcct ggcaccctgt gtgccaagac
     4141 gactggaacg agaactacgg gcgggcggcc tgcagggaca tgggctataa gaataatttt
     4201 tactctagcc aaggaatagt ggatgacagc ggatccacca gctttatgaa actgaacaca
     4261 agtgccggca atgtcgatat ctataaaaaa ctgtaccaca gtgatgcctg ttcttcaaaa
     4321 gcagtggttt ctttacgctg tatagcctgc ggggtcaact tgaactcaag ccgccagagc
     4381 aggatcgtgg gcggcgagag cgcgctcccg ggggcctggc cctggcaggt cagcctgcac
     4441 gtccagaacg tccacgtgtg cggaggctcc atcatcaccc ccgagtggat cgtgacagcc
     4501 gcccactgcg tggaaaaacc tcttaacaat ccatggcatt ggacggcatt tgcggggatt
     4561 ttgagacaat ctttcatgtt ctatggagcc ggataccaag tagaaaaagt gatttctcat
     4621 ccaaattatg actccaagac caagaacaat gacattgcgc tgatgaagct gcagaagcct
     4681 ctgactttca acgacctagt gaaaccagtg tgtctgccca acccaggcat gatgctgcag
     4741 ccagaacagc tctgctggat ttccgggtgg ggggccaccg aggagaaagg gaagacctca
     4801 gaagtgctga acgctgccaa ggtgcttctc attgagacac agagatgcaa cagcagatat
     4861 gtctatgaca acctgatcac accagccatg atctgtgccg gcttcctgca ggggaacgtc
     4921 gattcttgcc agggtgacag tggagggcct ctggtcactt cgaagaacaa tatctggtgg
     4981 ctgatagggg atacaagctg gggttctggc tgtgccaaag cttacagacc aggagtgtac
     5041 gggaatgtga tggtattcac ggactggatt tatcgacaaa tgagggcaga cggctaggga
     5101 tccatatgcg gaccgtacgt actagtggcc acggctgatc atacgggctg caggaattcc
     5161 gccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5221 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5281 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5341 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5401 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5461 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5521 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5581 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5641 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5701 tttcctttga aaaacacgat gataatacca tgaccgagta caagcccacg gtgcgcctcg
     5761 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5821 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     5881 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5941 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6001 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6061 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6121 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6181 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6241 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6301 ggtgcatgac ccgcaagccc ggtgcctgac gggcgcgtct ggaacttcga attcgatatc
     6361 aagcttatcg cgataccgtc gacctcgagg gaattccgat aatcaacctc tggattacaa
     6421 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6481 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6541 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6601 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6661 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6721 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6781 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6841 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6901 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6961 tcggatctcc ctttgggccg cctccccgca tcgggaattc gagctcggta cctttaagac
     7021 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7081 aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgg gaataacttc
     7141 gtatagcata cattatacga agttatgctg ctttttgctt gtactgggtc tctctggtta
     7201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     7381 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     7441 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     7501 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     7561 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     7621 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     7681 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     7741 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     7801 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     7861 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     7921 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7981 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8041 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8101 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     8161 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     8221 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8281 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8341 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8401 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8461 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8521 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8581 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8641 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8701 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8761 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8821 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8881 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8941 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9001 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9061 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9121 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     9181 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9241 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9301 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9361 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9421 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     9481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9841 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9901 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9961 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    10021 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    10081 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    10141 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    10201 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    10261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10321 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10381 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10441 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10501 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
    10561 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    10621 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    10681 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    10741 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    10801 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
    10861 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
    10921 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
    10981 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
    11041 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
    11101 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
    11161 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
    11221 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
    11281 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
    11341 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
    11401 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
    11461 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
    11521 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
    11581 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
    11641 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
    11701 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
    11761 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
    11821 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
    11881 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
    11941 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
    12001 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
    12061 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
    12121 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
    12181 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    12241 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    12301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    12421 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    12481 ggtttc
//
