LOCUS       sequence-309464-        8050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mCherry_pBirA-HA_FKBP8/biochemical assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Kwak C, Shin S, Park JS, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH,
            Park SK, Mun JY, Kim JS, Rhee HW
  TITLE     Contact-ID, a tool for profiling organelle contact sites, reveals 
            regulatory proteins of mitochondrial-associated membrane formation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117.
  PUBMED    32414919
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 
            10.1073/pnas.1916584117."
COMMENT     Sequence Label: pCDNA5/mCherry_pBirA-HA_FKBP8
FEATURES             Location/Qualifiers
     source          1..8050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             762..1469
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(907..925)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1175..1194
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(1482..1829,1830..1832,1833..2441)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             2445..2471
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3768..3785)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3774..3998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4131..4150)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4282..4329
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4337..5359
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4337..5359
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5489..5610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5526..5545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5580..5599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5659..5675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5659..5675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5672..5694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5683..5699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5707..5713,5714..5731,5732..5737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5752..5773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5890..5907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6061..6649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6141..6160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6820..7611,7612..7680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7443..7462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7681..7785)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7860..7879)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccatcga tatggtgagc aagggcgagg
      781 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      841 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      901 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      961 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1021 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1081 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1141 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1201 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1261 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     1321 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     1381 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1441 ccaccggcgg catggacgag ctgtacaagg aattcggatc caaggacaac accgtgcccc
     1501 tgaagctgat cgccctgctg gccaacggcg agttccactc tggcgagcag ctgggagaga
     1561 ccctgggaat gagcagagcc gccatcaaca agcacatcca gacactgaga gactggggag
     1621 tggacgtgtt caccgtgcct ggcaagggct acagcctgcc tgagcctatc cagctgctga
     1681 acgccaagca gatcctggga cagctggatg gcggaagcgt ggccgtgctg cctgtgatcg
     1741 actccaccaa tcagtacctg ctggacagaa tcggagagct gaagtccggc gacgcctgca
     1801 tcgccgagta ccagcaggct ggcagaggag gcagaggacg gaagtggttc agcccattcg
     1861 gagccaacct gtacctgtcc atgttctgga gactggagca gggacctgct gctgccatcg
     1921 gactgagtct ggtgatcgga atcgtgatgg ccgaggtgct gagaaagctg ggagccgaca
     1981 aggtgagagt gaagtggcct aatgacctgt acctccagga ccgcaagctg gctggcatcc
     2041 tggtggagct gacaggcaag acaggcgatg ccgctcagat cgtgatcgga gccggaatca
     2101 acatggccat gagaagagtg gaggagagcg tggtgaacca gggctggatc accctgcagg
     2161 aggctggcat caacctggac cggaacaccc tggccgccat gctgatcaga gagctgagag
     2221 ccgctctgga gctgttcgag caggagggac tggctcctta cctgagcaga tgggagaagc
     2281 tggacaactt catcaacaga cctgtgaagc tgatcatcgg cgacaaggaa atcttcggca
     2341 tctccagagg aatcgacaag cagggagctc tgctgctgga gcaggacgga atcatcaagc
     2401 cctggatggg cggagaaatc tccctgagaa gcgcagagaa ggcttaccct tacgatgtac
     2461 cggattacgc agcggccgca atggcatcgt gtgctgaacc ctctgagccc tctgccccac
     2521 tgcccgccgg ggtcccaccg ctcgaggact tcgaggtact ggatggggtt gaggatgcag
     2581 agggtgagga ggaagaggag gaggaagagg aggaagagga tgacctgagt gagctgccac
     2641 cgctggagga catgggacaa cccccggcgg aggaggctga gcagcctggg gccctggccc
     2701 gagagttcct tgctgccatg gagcccgagc ccgccccagc cccggcccca gaagagtggc
     2761 tggacattct ggggaacggg ctgttgagga agaagacgct ggtcccaggg ccgccaggtt
     2821 cgagccgccc ggtcaagggc caggtggtca ccgtacatct gcagacgtcg ctggagaatg
     2881 gcacacgggt gcaggaggag ccggagctgg tgttcactct gggtgactgt gacgtcatcc
     2941 aggccctgga tctcagtgtc ccactcatgg acgtggggga gacggccatg gtcactgctg
     3001 actccaagta ctgctacggc ccccaaggca gcaggagccc atacatcccc ccgcacgcgg
     3061 ccctgtgcct ggaggtgacc ctgaagacgg ctgtggacgg gcctgacctg gagatgctca
     3121 cggggcagga gcgcgtggcc ctggccaacc ggaagcggga gtgcggcaac gcccactacc
     3181 agcgggcgga cttcgtcctg gccgccaact cctacgacct cgccatcaag gctatcacct
     3241 ccagcgccaa agtggacatg acgttcgagg aggaggcaca gctcctgcag ttgaaggtga
     3301 agtgtctgaa caacctggcg gcctcgcagc tgaagctcga ccactaccgc gcagccctgc
     3361 gctcctgcag ccttgtgctg gagcaccagc cagacaacat caaggctctc ttccgcaagg
     3421 gcaaggtgct ggcccagcag ggggagtaca gtgaggccat ccccatcctg agggcagccc
     3481 tgaagctgga accttccaac aagacgatcc acgcagagct ctcaaagctg gtgaagaagc
     3541 atgcggcgca gcggagcacg gagaccgcct tgtaccggaa aatgctgggc aaccccagcc
     3601 ggctgcctgc taagtgccct ggcaagggtg cctggtccat cccatggaag tggctgtttg
     3661 gggcgactgc tgttgccttg gggggtgtgg cactctctgt ggtcatcgct gccaggaact
     3721 agggcgcgcc ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3781 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3841 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3901 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3961 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4021 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4081 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4141 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4201 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4261 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     4321 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     4381 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     4441 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     4501 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     4561 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     4621 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     4681 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     4741 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     4801 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     4861 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     4921 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     4981 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     5041 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     5101 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     5161 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     5221 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     5281 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     5341 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     5401 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5461 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5521 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5581 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5641 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5701 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5761 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5881 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5941 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6781 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6841 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6901 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6961 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7021 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7081 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7141 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7201 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7261 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7321 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7381 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7441 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7501 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7561 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7621 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7681 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7741 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7801 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     7861 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     7921 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     7981 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     8041 tatacgcgtt
//
