LOCUS       sequence-309474-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Specific gRNA against mouse IG-DMR cloned in the pX330 backbone 
            (Addgene Number 42230). Deletion of IG-DMR in mouse ESC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Aizawa E, Dumeau CE, Freimann R, Di Minin G, Wutz A
  TITLE     Polyploidy of semi-cloned embryos generated from parthenogenetic 
            haploid embryonic stem cells.
  JOURNAL   PLoS One. 2020 Sep 10;15(9):e0233072. doi: 
            10.1371/journal.pone.0233072. eCollection 2020.
  PUBMED    32911495
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-09-10"; volume: "15(9):e0233072. doi"; pages: " 
            10.1371/journal.pone.0233072. eCollection 2020"
COMMENT     Sequence Label: pX330_gRNA_IG-DMR-2
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..146
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        225..300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        397..682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 caggctgtta gagagwtaat tggaattaat ttgactgtaa acacaaagat attagtacaa
       61 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      121 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      181 atcttgtgga aaggacgaaa caccctgctt agaggtacta cgctgtttta gagctagaaa
      241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt
      361 gcgccaattc tgcagacaaa tggctctaga ggtacccgtt acataactta cggtaaatgg
      421 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaatagtaa cgccaatagg
      481 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      541 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      601 ctggcattgt gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      661 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      721 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      781 gatgggggcg gggggggggg gggggnccgc gcgccaggcg gggcggggcg gggcgagggg
      841 cgggg
//
