LOCUS       sequence-309475-       10531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of HO endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG646_lexO-HO_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..10531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(390..409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(391..409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(416..433)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(416..432)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(425..447)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3069..3173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3174..3242,3243..4034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3392..3411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4213..4801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4702..4721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4955..4972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5231..5247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5236..5258
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5255..5271
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5255..5271
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5292..5310
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(5473..5722)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(6186..7076)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(6189..7124)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(join(7143..7496,7497..7748))
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     complement(7184..7205)
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     terminator      complement(8418..8667)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(8717..8740)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9344..9355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtatagaag tatagtaatt tatgctgcaa aggtcctaat gtataaggaa agaagagaaa
       61 agaagaaaac aagagtttta tatacataca gagcacatgc tctagaccct gggtgtatat
      121 acagttatgc tgtgagtata tacagcacct agaccctggg tgtatataca gttatgctgt
      181 gagtatatac agcacctaga ccctgggtgt atatacagtt atgctgtgag tatatacagc
      241 acctagaccc tgggtgtata tacagttatg ctgtgagtat atacagcacc tagggagctc
      301 caattcgccc tataggcggc cgcgaaaaga aatatagtct tcaaggttat attatacagg
      361 tttatatatt attctgtttc tccaattcgc cctatagtga gtcgtattac aattcactgg
      421 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      481 cagcacatcc gatcgctgtg aagatcccag caaaggctta caaagtgtta tctcttttga
      541 gacttgttga gttgaacact ggtgttttca tcaaacttac caaggacgtg tacccattgt
      601 tgaaacttgt atcaccatat attgttatcg gacaaccttc acttgcatct atccgttctt
      661 taatccaaaa gagaactaga ataatgtggc aaaggccaga agataaagaa ccaaaagaga
      721 taatcttgaa tgacaacaat atcgttgaag agaaattagg tgatgaaggt gtcatttgta
      781 tcgaggaaat catccatgag atttcgacgt tgggcgaaaa tttctcgaaa tgtactttct
      841 tcctattacc attcaaattg aacagagaag tcagtggatt cggtgccatc tcccgtttga
      901 ataaactgaa aatgcgcgaa caaaacaaga agactcgtca aatttcaaac gctgccacgg
      961 ctccagttat ccaagtagac atcgactcaa tgatttccaa gttgaattga ttaactataa
     1021 aaggaaaata tctgtacaat agacatcggg ctcccattgg ccctacccac agatgtagaa
     1081 atacattact ctattcacta ctgcatttag ttatgtttaa catttgatat agcagacgct
     1141 accgccaggc acaatatatt ccccttccct cttgccattc gctgtacttg tggtagattc
     1201 caattcagcg cagtcacgtg ctagtaatca ccgcattttt ttcttttcct ttcaggctaa
     1261 aaccggttcc gggcctgatc cctgcactca ttttctaacg gaaaaccttc agaagcataa
     1321 ctacccattc cagtttagag tcatgacagg ttcaacatca gatgcttcat atacttttat
     1381 atattgaatt atataaatat atctatgtac tctaagtaag tacatctgct ttaacgcatt
     1441 cctacatttg cttcgattta tttttattgt tgatacctat ttgaagaagt aaaaagtatc
     1501 ccacactaca cagattatac catgtctaag aatatcgttg tcctaccggg tgatcacgtc
     1561 ggtaaagaag ttactgacga agctattaag gtcttgaatg ccattgctga agtccgtcca
     1621 gaaattaagt tcaatttcca acatcacttg atcgggggtg ctgccatcga tgccactggc
     1681 actcctttac cagatgaagc tcttgaagcc tctaagaaag ccgatgctgt cttactaggt
     1741 gctgttggtg gtccaaaatg gggtacgggc gcagttagac cagaacaagg tctattgaag
     1801 atcagaaagg aattgggtct atacgccaac ttgagaccat gtaactttgc ttctgattct
     1861 ttactagatc tttctccttt gaagcctgaa tatgcaaagg gtactgattt cgtcgtcgtt
     1921 agagaattgg ttggtggtat ctactttggt gaaagaaaag aagatgaagg tgacggagtt
     1981 gcttgggact ctgagaaata cagtgttcct gaagttcaaa gaattacaag aatggctgct
     2041 ttcttggcat tgcaacaaaa cccaccatta ccaatctggt ctcttgacaa ggctaacgtg
     2101 cttgcctctt ccagattgtg gagaaagact gttgaagaaa ccatcaagac tgagttccca
     2161 caattaactg ttcagcacca attgatcgac tctgctgcta tgattttggt taaatcacca
     2221 actaagctaa acggtgttgt tattaccaac aacatgtttg gtgatattat ctccgatgaa
     2281 gcctctgtta ttccaggttc tttgggttta ttaccttctg catctctagc ttccctacct
     2341 gacactaaca aggcattcgg tttgtacgaa ccatgtcatg gttctgcccc agatttacca
     2401 gcaaacaagg ttaacccaat tgctaccatc ttatctgcag ctatgatgtt gaagttatcc
     2461 ttggatttgg ttgaagaagg tagggctctt gaagaagctg ttagaaatgt cttggatgca
     2521 ggtgtcagaa ccggtgacct tggtggttct aactctacca ctgaggttgg cgatgctatc
     2581 gccaaggctg tcaaggaaat cttggcttaa agagtctttt gtaacgaccc cgtctccacc
     2641 aacttggtat gcttgaaatc tcaaggccat tacacattca gttatgtgaa cgaaaggtct
     2701 ttatttaacg tagcataaac taaataatac aggttccggt tagcctgcgg atcatatgcg
     2761 acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac
     2821 atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat
     2881 actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga
     2941 ggagggcacc acacaaaaag ttaggcgcgc cagagttaat taatagcagt tcgcaaaatc
     3001 gttcgcttcg caaatgttgc taatgccgcg ttctttcgac gtcaggtggc acttttcggg
     3061 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3121 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3301 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacc atcggaggac
     3601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4021 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4081 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtaggttta aacaaaagat
     4201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcctgca
     5041 ggaagaggcg cgccaagatg caaaccgaat aagtatttca aagttgcatt tgccttagcc
     5101 gtcctgacac cattggctat ttggatattt tatattgact ttcgtgtaca ttgatcacat
     5161 cgactgttct attggcaaat gaaccacggg cattgactat ttttcaggtt actactatat
     5221 attacagctg ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     5281 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tgggtacaag tgacaatata
     5341 tatatatata tataataacg acatcgttat ctgtaaatac ggccgaagct gggtaccaag
     5401 cagacgctac taaggaaaac aacaacggaa gcgatgatga gagcgacgat gagagtgtaa
     5461 actgcgaacc ttgcaaatta aagccttcga gcgtcccaaa accttctcaa gcaaggtttt
     5521 cagtataatg ttacatgcgt acacgcgttt gtacagaaaa aaaagaaaaa tttgaaatat
     5581 aaataacgtt cttaatacta acataactat taaaaaaaat aaatagggac ctagacttca
     5641 ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg gaggagggcg tgaatgtaag
     5701 cgtgacataa ctaattacat gatatagaca aaggaaaagg ggcctgtctc gaggataagc
     5761 tttaaacgcg tttaaagctt gaaacacaaa tcagtaccac cagaatgtct ttcagctcta
     5821 aattcaccag aggatcttct ttcagcaact ggatcgaaat catcagcaga tggaatagct
     5881 tcttcgaatt tttccaaacc agaatcgaaa ttaactttag catctttaac agtcaaagtt
     5941 tcccaaatca aatcagattg ttgttccaaa acaccaatag cttcacccaa agaagcaaca
     6001 gcagtatcca atttattaac agaacctgga gcatcgaaaa ccaattcagc aataccatct
     6061 tccaaccaat ccaaatacaa agtatcacct ttatacaaca tatcagtttc ttggattctc
     6121 aaagtaatac ctggaaattc gcccgcggtg ctgttatcgt tgcccggcag atcctgcatg
     6181 atatcgactg tggcagggaa accctctgcc tcccccgtga tgtaatactt ttgcaaggaa
     6241 tgcgatgaag tagagcccgc agtggccaag tggctttggt ccgtctcctc cacggatgcc
     6301 cctccacggc tagtgggcgc atgtaggcgg tgggcgtcca gcatctccag cagcaggtca
     6361 tagaggggca ccacgttctt gcacttcatg ctgtacagat gctccatgcc tttgttactc
     6421 atgtgcctga tgtgggagag gatgaggagg agctgggcca gccgctggtg ctgctgctgc
     6481 agggtcaggc ctgccttggc catcaggtgg atcaaagtgt ctgtgatctt gtccaggact
     6541 cggtggatat ggtccttctc ttccagagac ttcagggtgc tggacagaaa tgtgtacact
     6601 ccagaattaa gcaaaataat agatttgagg cacacaaact cctctccctg cagattcatc
     6661 atgcggaacc gagatgatgt agccagcagc atgtcgaaga tctccaccat gccctctaca
     6721 cattttccct ggttcctgtc caagagcaag ttaggagcaa acagtagctt cactgggtgc
     6781 tccatggagc gccagacgag accaatcatc aggatctcta accaggcaca ttctagaagg
     6841 tggacctgat catggagggt caaatccaca aagcctggca ccctcttcgc ccagttgatc
     6901 atgtgaacca gctccctgtc tgccaggttg gtcagtaagc ccatcatcga agcttcactg
     6961 aagggtctgg taggatcata ctcggaatag agtatggggg gctcagcatc caacaaggca
     7021 ctgaccatct ggtcggccgt cagggacaag gccaggctgt tcttcttaga gcgtttgatc
     7081 atgagcgggc ttggccaaag gttggcagct ctcatgtctc cagcagagat atccttagaa
     7141 cccagccagt cgccgttgcg aataacccca accgccagcc cttcaatggt gaagctctgc
     7201 tgacgaagat ctacgacaat tggtttaaac tcgctatttt ctggcaacag ttcgacttta
     7261 ttgccctgtt ttttcaggcg cttaacggta acctcgtcat caatacgtgc gacaacgacc
     7321 tgaccgttac gtacatcctg agttttatgc actgccagca agtcaccatc cataatgccg
     7381 atgtctttca tcgacatccc gctgacgcgc agcaggaaat cagcattcgg cttgaataag
     7441 gaaggatcga cctgataatg accttcaata tgctgttgcg ccagaagtgg ttcaccggca
     7501 gccacacgac ctaccagcgg caacccttct tcctcttcct gcaacagacg aatcccgcgt
     7561 gatgcgccgg aaacaatttc aataacgcct ttgcgtgcca gcgccttcag atgttcttca
     7621 gccgcgtttg gggaacggaa ccccaaacgc tgcgcgattt ccgcacgcgt cggcggcata
     7681 cctgtctggc tgatgtgatc acggatgaga tcaaacacct cttgttgcct ggccgttaac
     7741 gctttcatga attcctgcag cccgggggat ccactagttg ttaattcagt aaattttcga
     7801 tcttgggaag aaaaaagcag taagcgtgaa aaatctaaaa gctgatgtag tagaagatcc
     7861 tattctttaa caaagattga ccttttcttt ttcttcttgg tttgagtaga aaggggaagg
     7921 aagaatacaa gagagaggaa aaaaaggaag ataaaaagag agcgtgatat aaatgaatat
     7981 atattaaaca agagagattg ggaaggaaag gatcaaacaa acccaaaaat atttcaaaaa
     8041 ggagagagag aggcgagttt ggtttcaaaa cggtttattt atttatgcaa gaggacgtgg
     8101 aagaaaaaga agaaggaaga aaaaaatttg aaagaaaaaa acgcgtggcg ggtaaagaag
     8161 aaaatggaaa atagaggccg ggtgacagag aaatattgag ggttaattgg aaaatatgtt
     8221 agggtgaggc atatgttttt aagggttttg aggatccgat aaggaagaat gtaggttaaa
     8281 tgttgtgcat taattgctgt ggcagcttac ccgcttctct agagcggccg aagctgggta
     8341 ccaagcagac gctactaagg aaaacaacaa cggaagcgat gatgagagcg acgatgagag
     8401 tgtaaactgc gaaccttgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag
     8461 gttttcagta taatgttaca tgcgtacacg cgtttgtaca gaaaaaaaag aaaaatttga
     8521 aatataaata acgttcttaa tactaacata actattaaaa aaaataaata gggacctaga
     8581 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggagga gggcgtgaat
     8641 gtaagcgtga cataactaat tacatgatat agacaaagga aaaggggcct gtctcgaggt
     8701 tagttagtta aagcttcttg tcgtcgtcat ccttgtagtc gatatcgcag atgcgcgcac
     8761 ctgcgttgtt accacaactc ttatgaggcc cgcggacagc atcaaactgt aagattccgc
     8821 cacattttat acactctggt cctttaactg gcaaaccttc gggcgtaatg cccaattttt
     8881 cgcctttgtc ttttgccttt ttcacttcac gtgcttctgg tacatacttg caatttatac
     8941 agtgatgacc gctgaatttg tatcttccat agcatctagc acatactcga tttttaccac
     9001 tccaatcttt ataaaaatac ttgattccct ttctgggaca agcaacacag tgttttagat
     9061 tctttttttg tgatatttta tgctgttctc ccacacagca gcctcgacat gatttcactt
     9121 ctattttgtt gccaagcaag aaatttttat ggccttctat cgtaagccca tatacagtac
     9181 tctcaccctg gaaatcatcc gtgaagctga aatatacggg ttcccttttt ataattggcg
     9241 gaacttctct tgttttgtga ccacttcgac aatatgacaa aacattctgt aaagttgttc
     9301 ccccagcaac attacagtcg tatgtaaatt gacattggac ttttcttcct tcaatgattt
     9361 cctccctagc tgacctggtc gtcacagtag ctgacatacc aagagatctt gaaatatgga
     9421 caattccgtc cataatggat gaataaacag tttgaattgc tattttataa gattcagggc
     9481 cttcgccctt tttcacaacg tacccatctg agtcgatcaa gccggctaag aatgcttcac
     9541 gaacttctat atgctcgccg tacataaatt cagggatttg cttctctcca tcaagatccc
     9601 ttttaaactt taaaattgtg acagctttcc agaatggatt atttttcctc aaattccttg
     9661 tcttcctgtt ttcatctgga ccatctccat aatgaagcct tacatgtttg gcacgtagcg
     9721 gaacgtgatc gtcacaaacc gtaaggtaga gaccccagat tttcgcattt tctcttaaac
     9781 tctccattag cttaggatcc aagctatcta ctgagatttc tggctctttt gttgtaccgt
     9841 cacctaacca cagaccaagc atccaagcca tactttttac agcaggagtt acaaggtcac
     9901 tacgtccagt gagaaattta gataaaacac catttcctgt gagtactgga ccaaatctta
     9961 tgcagctaga aattctcaat tgagcatcaa gataatccaa atctctaact tcaatgtcaa
    10021 agttgaaata ttctccttta gagcgctcca tttcttctat gaagcgtttt gcggcaaact
    10081 caccttcaac tgtcattggg aatgtcttat gatggttttt tggaattatt attatcctac
    10141 catcaagcgt ctgacattgc tgcagatttc tccatctcac tttatatttg gtggcatttc
    10201 taccactttt ttccaacagt ggtttggtag ggaccctgac tgacaattta tgacctgcag
    10261 tacattgtaa tgcaagacgc tgataaactg ttctacgcct gggatctaac ctaccaggtt
    10321 caccttcaaa agctctgtgt ttggtttttt gctgtatatt atagattttc tgatagccct
    10381 gtgtgacatt tatgacgcgg gcagcggagc catctgcgca cataacgtaa gagttagccg
    10441 tgacgtttgc gatgtcttta atttcaccgt tagccatcag aatagtcgtg ttttcagaaa
    10501 gcatgaattc ctgcagcccg ggggatccac t
//
