LOCUS       sequence-309477-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AsiSI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG705_lexO-AsiSI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(389..408)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(390..408)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(415..432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(415..431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(424..446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3068..3172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3173..3241,3242..4033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3391..3410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4212..4800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4701..4720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4954..4971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5230..5246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5235..5257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5254..5270
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5254..5270
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5291..5309
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(5472..5721)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(6185..7075)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(6188..7123)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(join(7142..7495,7496..7747))
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     complement(7183..7204)
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     terminator      complement(8417..8666)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(8716..8739)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(8746..8766)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtatagaagt atagtaattt atgctgcaaa ggtcctaatg tataaggaaa gaagagaaaa
       61 gaagaaaaca agagttttat atacatacag agcacatgct ctagaccctg ggtgtatata
      121 cagttatgct gtgagtatat acagcaccta gaccctgggt gtatatacag ttatgctgtg
      181 agtatataca gcacctagac cctgggtgta tatacagtta tgctgtgagt atatacagca
      241 cctagaccct gggtgtatat acagttatgc tgtgagtata tacagcacct agggagctcc
      301 aattcgccct ataggcggcc gcgaaaagaa atatagtctt caaggttata ttatacaggt
      361 ttatatatta ttctgtttct ccaattcgcc ctatagtgag tcgtattaca attcactggc
      421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      481 agcacatccg atcgctgtga agatcccagc aaaggcttac aaagtgttat ctcttttgag
      541 acttgttgag ttgaacactg gtgttttcat caaacttacc aaggacgtgt acccattgtt
      601 gaaacttgta tcaccatata ttgttatcgg acaaccttca cttgcatcta tccgttcttt
      661 aatccaaaag agaactagaa taatgtggca aaggccagaa gataaagaac caaaagagat
      721 aatcttgaat gacaacaata tcgttgaaga gaaattaggt gatgaaggtg tcatttgtat
      781 cgaggaaatc atccatgaga tttcgacgtt gggcgaaaat ttctcgaaat gtactttctt
      841 cctattacca ttcaaattga acagagaagt cagtggattc ggtgccatct cccgtttgaa
      901 taaactgaaa atgcgcgaac aaaacaagaa gactcgtcaa atttcaaacg ctgccacggc
      961 tccagttatc caagtagaca tcgactcaat gatttccaag ttgaattgat taactataaa
     1021 aggaaaatat ctgtacaata gacatcgggc tcccattggc cctacccaca gatgtagaaa
     1081 tacattactc tattcactac tgcatttagt tatgtttaac atttgatata gcagacgcta
     1141 ccgccaggca caatatattc cccttccctc ttgccattcg ctgtacttgt ggtagattcc
     1201 aattcagcgc agtcacgtgc tagtaatcac cgcatttttt tcttttcctt tcaggctaaa
     1261 accggttccg ggcctgatcc ctgcactcat tttctaacgg aaaaccttca gaagcataac
     1321 tacccattcc agtttagagt catgacaggt tcaacatcag atgcttcata tacttttata
     1381 tattgaatta tataaatata tctatgtact ctaagtaagt acatctgctt taacgcattc
     1441 ctacatttgc ttcgatttat ttttattgtt gatacctatt tgaagaagta aaaagtatcc
     1501 cacactacac agattatacc atgtctaaga atatcgttgt cctaccgggt gatcacgtcg
     1561 gtaaagaagt tactgacgaa gctattaagg tcttgaatgc cattgctgaa gtccgtccag
     1621 aaattaagtt caatttccaa catcacttga tcgggggtgc tgccatcgat gccactggca
     1681 ctcctttacc agatgaagct cttgaagcct ctaagaaagc cgatgctgtc ttactaggtg
     1741 ctgttggtgg tccaaaatgg ggtacgggcg cagttagacc agaacaaggt ctattgaaga
     1801 tcagaaagga attgggtcta tacgccaact tgagaccatg taactttgct tctgattctt
     1861 tactagatct ttctcctttg aagcctgaat atgcaaaggg tactgatttc gtcgtcgtta
     1921 gagaattggt tggtggtatc tactttggtg aaagaaaaga agatgaaggt gacggagttg
     1981 cttgggactc tgagaaatac agtgttcctg aagttcaaag aattacaaga atggctgctt
     2041 tcttggcatt gcaacaaaac ccaccattac caatctggtc tcttgacaag gctaacgtgc
     2101 ttgcctcttc cagattgtgg agaaagactg ttgaagaaac catcaagact gagttcccac
     2161 aattaactgt tcagcaccaa ttgatcgact ctgctgctat gattttggtt aaatcaccaa
     2221 ctaagctaaa cggtgttgtt attaccaaca acatgtttgg tgatattatc tccgatgaag
     2281 cctctgttat tccaggttct ttgggtttat taccttctgc atctctagct tccctacctg
     2341 acactaacaa ggcattcggt ttgtacgaac catgtcatgg ttctgcccca gatttaccag
     2401 caaacaaggt taacccaatt gctaccatct tatctgcagc tatgatgttg aagttatcct
     2461 tggatttggt tgaagaaggt agggctcttg aagaagctgt tagaaatgtc ttggatgcag
     2521 gtgtcagaac cggtgacctt ggtggttcta actctaccac tgaggttggc gatgctatcg
     2581 ccaaggctgt caaggaaatc ttggcttaaa gagtcttttg taacgacccc gtctccacca
     2641 acttggtatg cttgaaatct caaggccatt acacattcag ttatgtgaac gaaaggtctt
     2701 tatttaacgt agcataaact aaataataca ggttccggtt agcctgcgga tcatatgcga
     2761 cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca
     2821 tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata
     2881 ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag
     2941 gagggcacca cacaaaaagt taggcgcgcc agagttaatt aatagcagtt cgcaaaatcg
     3001 ttcgcttcgc aaatgttgct aatgccgcgt tctttcgacg tcaggtggca cttttcgggg
     3061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacca tcggaggacc
     3601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtaggtttaa acaaaagatc
     4201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4321 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4921 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4981 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcctgcag
     5041 gaagaggcgc gccaagatgc aaaccgaata agtatttcaa agttgcattt gccttagccg
     5101 tcctgacacc attggctatt tggatatttt atattgactt tcgtgtacat tgatcacatc
     5161 gactgttcta ttggcaaatg aaccacgggc attgactatt tttcaggtta ctactatata
     5221 ttacagctgt tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     5281 ccaagctcga aattaaccct cactaaaggg aacaaaagct gggtacaagt gacaatatat
     5341 atatatatat ataataacga catcgttatc tgtaaatacg gccgaagctg ggtaccaagc
     5401 agacgctact aaggaaaaca acaacggaag cgatgatgag agcgacgatg agagtgtaaa
     5461 ctgcgaacct tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc
     5521 agtataatgt tacatgcgta cacgcgtttg tacagaaaaa aaagaaaaat ttgaaatata
     5581 aataacgttc ttaatactaa cataactatt aaaaaaaata aatagggacc tagacttcag
     5641 gttgtctaac tccttccttt tcggttagag cggatgtggg aggagggcgt gaatgtaagc
     5701 gtgacataac taattacatg atatagacaa aggaaaaggg gcctgtctcg aggataagct
     5761 ttaaacgcgt ttaaagcttg aaacacaaat cagtaccacc agaatgtctt tcagctctaa
     5821 attcaccaga ggatcttctt tcagcaactg gatcgaaatc atcagcagat ggaatagctt
     5881 cttcgaattt ttccaaacca gaatcgaaat taactttagc atctttaaca gtcaaagttt
     5941 cccaaatcaa atcagattgt tgttccaaaa caccaatagc ttcacccaaa gaagcaacag
     6001 cagtatccaa tttattaaca gaacctggag catcgaaaac caattcagca ataccatctt
     6061 ccaaccaatc caaatacaaa gtatcacctt tatacaacat atcagtttct tggattctca
     6121 aagtaatacc tggaaattcg cccgcggtgc tgttatcgtt gcccggcaga tcctgcatga
     6181 tatcgactgt ggcagggaaa ccctctgcct cccccgtgat gtaatacttt tgcaaggaat
     6241 gcgatgaagt agagcccgca gtggccaagt ggctttggtc cgtctcctcc acggatgccc
     6301 ctccacggct agtgggcgca tgtaggcggt gggcgtccag catctccagc agcaggtcat
     6361 agaggggcac cacgttcttg cacttcatgc tgtacagatg ctccatgcct ttgttactca
     6421 tgtgcctgat gtgggagagg atgaggagga gctgggccag ccgctggtgc tgctgctgca
     6481 gggtcaggcc tgccttggcc atcaggtgga tcaaagtgtc tgtgatcttg tccaggactc
     6541 ggtggatatg gtccttctct tccagagact tcagggtgct ggacagaaat gtgtacactc
     6601 cagaattaag caaaataata gatttgaggc acacaaactc ctctccctgc agattcatca
     6661 tgcggaaccg agatgatgta gccagcagca tgtcgaagat ctccaccatg ccctctacac
     6721 attttccctg gttcctgtcc aagagcaagt taggagcaaa cagtagcttc actgggtgct
     6781 ccatggagcg ccagacgaga ccaatcatca ggatctctaa ccaggcacat tctagaaggt
     6841 ggacctgatc atggagggtc aaatccacaa agcctggcac cctcttcgcc cagttgatca
     6901 tgtgaaccag ctccctgtct gccaggttgg tcagtaagcc catcatcgaa gcttcactga
     6961 agggtctggt aggatcatac tcggaataga gtatgggggg ctcagcatcc aacaaggcac
     7021 tgaccatctg gtcggccgtc agggacaagg ccaggctgtt cttcttagag cgtttgatca
     7081 tgagcgggct tggccaaagg ttggcagctc tcatgtctcc agcagagata tccttagaac
     7141 ccagccagtc gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct
     7201 gacgaagatc tacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat
     7261 tgccctgttt tttcaggcgc ttaacggtaa cctcgtcatc aatacgtgcg acaacgacct
     7321 gaccgttacg tacatcctga gttttatgca ctgccagcaa gtcaccatcc ataatgccga
     7381 tgtctttcat cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaataagg
     7441 aaggatcgac ctgataatga ccttcaatat gctgttgcgc cagaagtggt tcaccggcag
     7501 ccacacgacc taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg
     7561 atgcgccgga aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag
     7621 ccgcgtttgg ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac
     7681 ctgtctggct gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg
     7741 ctttcatgaa ttcctgcagc ccgggggatc cactagttgt taattcagta aattttcgat
     7801 cttgggaaga aaaaagcagt aagcgtgaaa aatctaaaag ctgatgtagt agaagatcct
     7861 attctttaac aaagattgac cttttctttt tcttcttggt ttgagtagaa aggggaagga
     7921 agaatacaag agagaggaaa aaaaggaaga taaaaagaga gcgtgatata aatgaatata
     7981 tattaaacaa gagagattgg gaaggaaagg atcaaacaaa cccaaaaata tttcaaaaag
     8041 gagagagaga ggcgagtttg gtttcaaaac ggtttattta tttatgcaag aggacgtgga
     8101 agaaaaagaa gaaggaagaa aaaaatttga aagaaaaaaa cgcgtggcgg gtaaagaaga
     8161 aaatggaaaa tagaggccgg gtgacagaga aatattgagg gttaattgga aaatatgtta
     8221 gggtgaggca tatgttttta agggttttga ggatccgata aggaagaatg taggttaaat
     8281 gttgtgcatt aattgctgtg gcagcttacc cgcttctcta gagcggccga agctgggtac
     8341 caagcagacg ctactaagga aaacaacaac ggaagcgatg atgagagcga cgatgagagt
     8401 gtaaactgcg aaccttgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg
     8461 ttttcagtat aatgttacat gcgtacacgc gtttgtacag aaaaaaaaga aaaatttgaa
     8521 atataaataa cgttcttaat actaacataa ctattaaaaa aaataaatag ggacctagac
     8581 ttcaggttgt ctaactcctt ccttttcggt tagagcggat gtgggaggag ggcgtgaatg
     8641 taagcgtgac ataactaatt acatgatata gacaaaggaa aaggggcctg tctcgaggtt
     8701 agttagttaa agcttcttgt cgtcgtcatc cttgtagtcg atatcaactt ttctcttctt
     8761 ctttggcaac ataacttgat cggtttcgtc gatcaagtgt ctttgtctat cagacaagat
     8821 caacctgtat ctggcggatc taataacttc aatttcggag gcaattggag tagcattaaa
     8881 ctccaacagg ttcagaacgg aatctctatc aaccttaatg gtcatgggtc tgaactcatg
     8941 ttcagcaaag tatctattgg cgaaagcaac ttgagtagca atttgaactc tgttaactgg
     9001 atcagcaaca gccttagctt caactggaat aataactgga acttcatcat ctctaacggc
     9061 aacagcaaca ttaacttcat ccaattcagc ttgaccaaca cctggaacag attttctaac
     9121 atgggacctc aatctgtaaa cggtcaaacc agtaaaatgt tgccacaagt tacagtaacc
     9181 agcaaccatc aaaatagctt gttcatccat atcagccaaa tgttctggca gcaatcttct
     9241 aactggattt ggagtcaaat ccaaggcttc aattggtcta gtgtttggca aatcaataac
     9301 ggtggaggag gcttgttcaa atctatatct acctctacca gcttgggtca aaacgtagaa
     9361 acccaattcc aaaatttcgg ctggcaaaac agttctggat ctcattctgt agataacatc
     9421 agcagcgttt ctaacaacga tacccaatgc agcagcagca tctctaatat catccaaggt
     9481 gaactcaaca acagtatcac cagtcttgta tctcaaagcg aaaacgtatc tcaagattgg
     9541 gacgtaaacc ttagaagtca aaccatgaga ttgcaacaaa cgaccatcac cagaaattgg
     9601 gttcaattga tcgatagatt cacccatgaa ttcctgcagc ccgggggatc cacta
//
