LOCUS       sequence-309478-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of SbfI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG706_lexO-SbfI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             998..1018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1025..1048
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1098..1347
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(2017..2268,2269..2622)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     2560..2581
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             2641..3576
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2689..3579
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      4043..4292
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(4455..4473)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4494..4510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4494..4510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4507..4529)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4518..4534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4793..4810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4964..5552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5044..5063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5731..6522,6523..6591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6354..6373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6592..6696)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9318..9340
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9332..9349
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9333..9349
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9356..9375
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9356..9374
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagtggatcc cccgggctgc aggaattcat gaactcctcc gatggtattg atggtactgt
       61 tgcttctatt gatactgcta gagctttgtt gaagagattc ggttttgatg cccaaagata
      121 caacgttaga tctgctgtta ctttgttggc tttggctggt ttgaaacctg gtgatagatg
      181 ggttgattct actactccaa gattgggtgt tcaaaagatc atggattggt ctggtgaaca
      241 ttgggctaaa ccatacgcta ctggttcaag agaagatttc agaaaaaaga ccttgagaca
      301 atgggttgac aatggttttg ctgttttgaa cgctgacaac ttgaatattg ctaccaactc
      361 tcagttgaac gagtactgtt tgtctgatga agccttgcaa gccttgagag cttatggtac
      421 tgaaggtttt gaagaatcct tggtcgtttt tttggacgaa gctagtaaag ctgttaaggc
      481 tagagctgaa gctctacaag ctgctatgat ttctgttgat ttgccaggtg gtgaagagtt
      541 tttgttgtct ccagcaggtc aaaacccact gttgaaaaaa atggtcgaag agttcgttcc
      601 aagatttgct ccaagatcta ctgtcttgta tttgggtgat accagaggta agcactcttt
      661 gttcgaaaga gaaatcttcg aggaagtttt gggtttgact tttgatccac atggtagaat
      721 gcctgatttg atcttgcatg atgaagttag aggttggctg tttttgatgg aagccgttaa
      781 gtctaaaggt ccattcgatg aagaaagaca cagatcattg caagagttgt tcgttactcc
      841 atctgccggt ttgattttcg ttaactgttt cgaaaaccgt gagtctatga gacagtggtt
      901 gccagaattg gcttgggaaa ctgaagcctg ggttgctgaa gatccagatc atttgattca
      961 tttgaacggc tccagatttt tgggtccata cgaaagacca aagaagaaga gaaaggtcga
     1021 tatcgactac aaggatgacg acgacaagaa gctttaacta actaacctcg agacaggccc
     1081 cttttccttt gtctatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
     1141 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     1201 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     1261 tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     1321 ttgggacgct cgaaggcttt aatttgcaag gttcgcagtt tacactctca tcgtcgctct
     1381 catcatcgct tccgttgttg ttttccttag tagcgtctgc ttggtaccca gcttcggccg
     1441 ctctagagaa gcgggtaagc tgccacagca attaatgcac aacatttaac ctacattctt
     1501 ccttatcgga tcctcaaaac ccttaaaaac atatgcctca ccctaacata ttttccaatt
     1561 aaccctcaat atttctctgt cacccggcct ctattttcca ttttcttctt tacccgccac
     1621 gcgttttttt ctttcaaatt tttttcttcc ttcttctttt tcttccacgt cctcttgcat
     1681 aaataaataa accgttttga aaccaaactc gcctctctct ctcctttttg aaatattttt
     1741 gggtttgttt gatcctttcc ttcccaatct ctcttgttta atatatattc atttatatca
     1801 cgctctcttt ttatcttcct ttttttcctc tctcttgtat tcttccttcc cctttctact
     1861 caaaccaaga agaaaaagaa aaggtcaatc tttgttaaag aataggatct tctactacat
     1921 cagcttttag atttttcacg cttactgctt ttttcttccc aagatcgaaa atttactgaa
     1981 ttaacaacta gtggatcccc cgggctgcag gaattcatga aagcgttaac ggccaggcaa
     2041 caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt
     2101 gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag
     2161 gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg
     2221 ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccactt
     2281 ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttatt caagccgaat
     2341 gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag acatcggcat tatggatggt
     2401 gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt
     2461 attgatgacg aggttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg
     2521 ccagaaaata gcgagtttaa accaattgtc gtagatcttc gtcagcagag cttcaccatt
     2581 gaagggctgg cggttggggt tattcgcaac ggcgactggc tgggttctaa ggatatctct
     2641 gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag
     2701 aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct
     2761 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg
     2821 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag
     2881 agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc
     2941 tggttagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta
     3001 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
     3061 gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
     3121 gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg
     3181 tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc
     3241 acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag
     3301 cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg
     3361 gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag
     3421 atgctggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag
     3481 acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat
     3541 tacatcacgg gggaggcaga gggtttccct gccacagtcg atatcatgca ggatctgccg
     3601 ggcaacgata acagcaccgc gggcgaattt ccaggtatta ctttgagaat ccaagaaact
     3661 gatatgttgt ataaaggtga tactttgtat ttggattggt tggaagatgg tattgctgaa
     3721 ttggttttcg atgctccagg ttctgttaat aaattggata ctgctgttgc ttctttgggt
     3781 gaagctattg gtgttttgga acaacaatct gatttgattt gggaaacttt gactgttaaa
     3841 gatgctaaag ttaatttcga ttctggtttg gaaaaattcg aagaagctat tccatctgct
     3901 gatgatttcg atccagttgc tgaaagaaga tcctctggtg aatttagagc tgaaagacat
     3961 tctggtggta ctgatttgtg tttcaagctt taaacgcgtt taaagcttat cctcgagaca
     4021 ggcccctttt cctttgtcta tatcatgtaa ttagttatgt cacgcttaca ttcacgccct
     4081 cctcccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     4141 atttattttt tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     4201 ttttttttct gtacaaacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     4261 aggttttggg acgctcgaag gctttaattt gcaaggttcg cagtttacac tctcatcgtc
     4321 gctctcatca tcgcttccgt tgttgttttc cttagtagcg tctgcttggt acccagcttc
     4381 ggccgtattt acagataacg atgtcgttat tatatatata tatatatatt gtcacttgta
     4441 cccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     4501 ctgtttcctg tgtgaaattg ttatccgctc acaacagctg taatatatag tagtaacctg
     4561 aaaaatagtc aatgcccgtg gttcatttgc caatagaaca gtcgatgtga tcaatgtaca
     4621 cgaaagtcaa tataaaatat ccaaatagcc aatggtgtca ggacggctaa ggcaaatgca
     4681 actttgaaat acttattcgg tttgcatctt ggcgcgcctc ttcctgcagg cattaatgaa
     4741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tgtttaaacc
     5581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatggttgt
     6181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6721 tgacgtcgaa agaacgcggc attagcaaca tttgcgaagc gaacgatttt gcgaactgct
     6781 attaattaac tctggcgcgc ctaacttttt gtgtggtgcc ctcctccttg tcaatattaa
     6841 tgttaaagtg caattctttt tccttatcac gttgagccat tagtatcaat ttgcttacct
     6901 gtattccttt acatcctcct ttttctcctt cttgataaat gtatgtagat tgcgtatata
     6961 gtttcgtcta ccctatgaac atattccatt ttgtaatttc gtgtcgcata tgatccgcag
     7021 gctaaccgga acctgtatta tttagtttat gctacgttaa ataaagacct ttcgttcaca
     7081 taactgaatg tgtaatggcc ttgagatttc aagcatacca agttggtgga gacggggtcg
     7141 ttacaaaaga ctctttaagc caagatttcc ttgacagcct tggcgatagc atcgccaacc
     7201 tcagtggtag agttagaacc accaaggtca ccggttctga cacctgcatc caagacattt
     7261 ctaacagctt cttcaagagc cctaccttct tcaaccaaat ccaaggataa cttcaacatc
     7321 atagctgcag ataagatggt agcaattggg ttaaccttgt ttgctggtaa atctggggca
     7381 gaaccatgac atggttcgta caaaccgaat gccttgttag tgtcaggtag ggaagctaga
     7441 gatgcagaag gtaataaacc caaagaacct ggaataacag aggcttcatc ggagataata
     7501 tcaccaaaca tgttgttggt aataacaaca ccgtttagct tagttggtga tttaaccaaa
     7561 atcatagcag cagagtcgat caattggtgc tgaacagtta attgtgggaa ctcagtcttg
     7621 atggtttctt caacagtctt tctccacaat ctggaagagg caagcacgtt agccttgtca
     7681 agagaccaga ttggtaatgg tgggttttgt tgcaatgcca agaaagcagc cattcttgta
     7741 attctttgaa cttcaggaac actgtatttc tcagagtccc aagcaactcc gtcaccttca
     7801 tcttcttttc tttcaccaaa gtagatacca ccaaccaatt ctctaacgac gacgaaatca
     7861 gtaccctttg catattcagg cttcaaagga gaaagatcta gtaaagaatc agaagcaaag
     7921 ttacatggtc tcaagttggc gtatagaccc aattcctttc tgatcttcaa tagaccttgt
     7981 tctggtctaa ctgcgcccgt accccatttt ggaccaccaa cagcacctag taagacagca
     8041 tcggctttct tagaggcttc aagagcttca tctggtaaag gagtgccagt ggcatcgatg
     8101 gcagcacccc cgatcaagtg atgttggaaa ttgaacttaa tttctggacg gacttcagca
     8161 atggcattca agaccttaat agcttcgtca gtaacttctt taccgacgtg atcacccggt
     8221 aggacaacga tattcttaga catggtataa tctgtgtagt gtgggatact ttttacttct
     8281 tcaaataggt atcaacaata aaaataaatc gaagcaaatg taggaatgcg ttaaagcaga
     8341 tgtacttact tagagtacat agatatattt atataattca atatataaaa gtatatgaag
     8401 catctgatgt tgaacctgtc atgactctaa actggaatgg gtagttatgc ttctgaaggt
     8461 tttccgttag aaaatgagtg cagggatcag gcccggaacc ggttttagcc tgaaaggaaa
     8521 agaaaaaaat gcggtgatta ctagcacgtg actgcgctga attggaatct accacaagta
     8581 cagcgaatgg caagagggaa ggggaatata ttgtgcctgg cggtagcgtc tgctatatca
     8641 aatgttaaac ataactaaat gcagtagtga atagagtaat gtatttctac atctgtgggt
     8701 agggccaatg ggagcccgat gtctattgta cagatatttt ccttttatag ttaatcaatt
     8761 caacttggaa atcattgagt cgatgtctac ttggataact ggagccgtgg cagcgtttga
     8821 aatttgacga gtcttcttgt tttgttcgcg cattttcagt ttattcaaac gggagatggc
     8881 accgaatcca ctgacttctc tgttcaattt gaatggtaat aggaagaaag tacatttcga
     8941 gaaattttcg cccaacgtcg aaatctcatg gatgatttcc tcgatacaaa tgacaccttc
     9001 atcacctaat ttctcttcaa cgatattgtt gtcattcaag attatctctt ttggttcttt
     9061 atcttctggc ctttgccaca ttattctagt tctcttttgg attaaagaac ggatagatgc
     9121 aagtgaaggt tgtccgataa caatatatgg tgatacaagt ttcaacaatg ggtacacgtc
     9181 cttggtaagt ttgatgaaaa caccagtgtt caactcaaca agtctcaaaa gagataacac
     9241 tttgtaagcc tttgctggga tcttcacagc gatcggatgt gctgcaaggc gattaagttg
     9301 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     9361 cgactcacta tagggcgaat tggagaaaca gaataatata taaacctgta taatataacc
     9421 ttgaagacta tatttctttt cgcggccgcc tatagggcga attggagctc cctaggtgct
     9481 gtatatactc acagcataac tgtatataca cccagggtct aggtgctgta tatactcaca
     9541 gcataactgt atatacaccc agggtctagg tgctgtatat actcacagca taactgtata
     9601 tacacccagg gtctaggtgc tgtatatact cacagcataa ctgtatatac acccagggtc
     9661 tagagcatgt gctctgtatg tatataaaac tcttgttttc ttcttttctc ttctttcctt
     9721 atacattagg acctttgcag cataaattac tatacttcta tac
//
