LOCUS       sequence-309480-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AscI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG711_lexO-AscI_LexA-TF_HIS3MX
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1790..1810
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1817..1840
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1890..2139
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(2809..3060,3061..3414)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3352..3373
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3433..4368
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3481..4371
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      4835..5084
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5247..5265)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5286..5302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5286..5302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5299..5321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5310..5326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(5560..5577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5731..6319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5811..5830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6498..7289,7290..7358))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7121..7140
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7359..7463)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7761..8411
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     primer_bind     8750..8772
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8764..8781
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8765..8781
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8788..8799,1..8)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(8788..8799,1..7)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatagggcg aattggagaa acagaataat atataaacct gtataatata accttgaaga
       61 ctatatttct tttcgcggcc gcctataggg cgaattggag ctccctaggt gctgtatata
      121 ctcacagcat aactgtatat acacccaggg tctaggtgct gtatatactc acagcataac
      181 tgtatataca cccagggtct aggtgctgta tatactcaca gcataactgt atatacaccc
      241 agggtctagg tgctgtatat actcacagca taactgtata tacacccagg gtctagagca
      301 tgtgctctgt atgtatataa aactcttgtt ttcttctttt ctcttctttc cttatacatt
      361 aggacctttg cagcataaat tactatactt ctatactagt ggatcccccg ggctgcagga
      421 attcatgatt gaatttccag agtacagaga ttcttctgct gctccaaaaa tctccgattt
      481 ggaaagattg atggacaggt ctttgaccaa ctctcaatat gttgatggtg ctaacgctgc
      541 tagattattg ggtactttca tcagatcctt cagatccgtt attggttctg ctgaagaatc
      601 tgctactaga gctaatttgg ttgaagctca tgatgaagct aagttgttcg gtttgatgtt
      661 gtctgctggt ttcgatttga tctgtaacgc tgaatacgtt cacggtagat tggttaacaa
      721 caagtggatc tattgtcaca gaggtggtga accagcagtt gcttattact ctttcttgaa
      781 acaatgtcca agatgctgct tggatagagg tttggaaggt agattgtctg gtgctcaaca
      841 taagccaact tctcatcata ttggtgaaat taccaccgtt gctattgcct tgttgttgca
      901 attagttgct gctgctaacg aaaacccatt cgaaattgct actatcacca agcaatccca
      961 tgatgttgat gctattggtt tcagagatga cttgttggtc ttgttcgaaa tcaaggcttc
     1021 tccaatggtt tctttcccat tggttactga attggaagaa cctatgttgc aagaaggtcc
     1081 agacggtcca gttgagtata gacaacattc tttggttgac ttgacgttgc agggtagaga
     1141 atttgctgtt gctattccac atgctgaaac agctattcca ttgggtgaaa gagaaggcga
     1201 atcttggcca tacgaaccat tgattgatta cttttctgtt ccagctaatg ccgcttctta
     1261 cttgcaagcc tggattgaat tatatgctgc ttacagaact cccaagactc aaagagctgg
     1321 tagaactgct gctttggctt atttggttaa tggttggggt gacgaaatcg attctaacaa
     1381 aacaaaacca ggtttgggta gaaccgatga tgttaagaaa ggtacttacc agttgttgaa
     1441 gttcggttcc tattacagag atgatgctgc atcagttcca gttagaggtg ctttggttgc
     1501 taacttagat cctttgtttt tgaggccagg ttacatcgat ggtttgtctg atgttagatg
     1561 gggtcatggt agagatttca ctttggaaga aggtgagtac agaattgctg aaggttcttt
     1621 gagacacttg tacgatgcta ttttggcttt caatgaccct ttgttgaatg acccattgct
     1681 gcaagaaatc tttgatttgg gtgctgtcga aagaaagttg gctaatggtg atttggaagc
     1741 cttgttggat aagtggattg ctagaccaga aatagttttg gacccatctc caaagaagaa
     1801 gagaaaagtt gatatcgact acaaggatga cgacgacaag aagctttaac taactaacct
     1861 cgagacaggc cccttttcct ttgtctatat catgtaatta gttatgtcac gcttacattc
     1921 acgccctcct cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
     1981 ggtccctatt tatttttttt aatagttatg ttagtattaa gaacgttatt tatatttcaa
     2041 atttttcttt tttttctgta caaacgcgtg tacgcatgta acattatact gaaaaccttg
     2101 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca aggttcgcag tttacactct
     2161 catcgtcgct ctcatcatcg cttccgttgt tgttttcctt agtagcgtct gcttggtacc
     2221 cagcttcggc cgctctagag aagcgggtaa gctgccacag caattaatgc acaacattta
     2281 acctacattc ttccttatcg gatcctcaaa acccttaaaa acatatgcct caccctaaca
     2341 tattttccaa ttaaccctca atatttctct gtcacccggc ctctattttc cattttcttc
     2401 tttacccgcc acgcgttttt ttctttcaaa tttttttctt ccttcttctt tttcttccac
     2461 gtcctcttgc ataaataaat aaaccgtttt gaaaccaaac tcgcctctct ctctcctttt
     2521 tgaaatattt ttgggtttgt ttgatccttt ccttcccaat ctctcttgtt taatatatat
     2581 tcatttatat cacgctctct ttttatcttc ctttttttcc tctctcttgt attcttcctt
     2641 cccctttcta ctcaaaccaa gaagaaaaag aaaaggtcaa tctttgttaa agaataggat
     2701 cttctactac atcagctttt agatttttca cgcttactgc ttttttcttc ccaagatcga
     2761 aaatttactg aattaacaac tagtggatcc cccgggctgc aggaattcat gaaagcgtta
     2821 acggccaggc aacaagaggt gtttgatctc atccgtgatc acatcagcca gacaggtatg
     2881 ccgccgacgc gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa
     2941 gaacatctga aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc
     3001 gggattcgtc tgttgcagga agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc
     3061 ggtgaaccac ttctggcgca acagcatatt gaaggtcatt atcaggtcga tccttcctta
     3121 ttcaagccga atgctgattt cctgctgcgc gtcagcggga tgtcgatgaa agacatcggc
     3181 attatggatg gtgacttgct ggcagtgcat aaaactcagg atgtacgtaa cggtcaggtc
     3241 gttgtcgcac gtattgatga cgaggttacc gttaagcgcc tgaaaaaaca gggcaataaa
     3301 gtcgaactgt tgccagaaaa tagcgagttt aaaccaattg tcgtagatct tcgtcagcag
     3361 agcttcacca ttgaagggct ggcggttggg gttattcgca acggcgactg gctgggttct
     3421 aaggatatct ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc
     3481 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc
     3541 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt
     3601 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc
     3661 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt
     3721 ctagaatgtg cctggttaga gatcctgatg attggtctcg tctggcgctc catggagcac
     3781 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta
     3841 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg
     3901 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg
     3961 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc
     4021 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag
     4081 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt
     4141 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac
     4201 ctgctgctgg agatgctgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca
     4261 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc
     4321 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagt cgatatcatg
     4381 caggatctgc cgggcaacga taacagcacc gcgggcgaat ttccaggtat tactttgaga
     4441 atccaagaaa ctgatatgtt gtataaaggt gatactttgt atttggattg gttggaagat
     4501 ggtattgctg aattggtttt cgatgctcca ggttctgtta ataaattgga tactgctgtt
     4561 gcttctttgg gtgaagctat tggtgttttg gaacaacaat ctgatttgat ttgggaaact
     4621 ttgactgtta aagatgctaa agttaatttc gattctggtt tggaaaaatt cgaagaagct
     4681 attccatctg ctgatgattt cgatccagtt gctgaaagaa gatcctctgg tgaatttaga
     4741 gctgaaagac attctggtgg tactgatttg tgtttcaagc tttaaacgcg tttaaagctt
     4801 atcctcgaga caggcccctt ttcctttgtc tatatcatgt aattagttat gtcacgctta
     4861 cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag acaacctgaa
     4921 gtctaggtcc ctatttattt tttttaatag ttatgttagt attaagaacg ttatttatat
     4981 ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa
     5041 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaaggtt cgcagtttac
     5101 actctcatcg tcgctctcat catcgcttcc gttgttgttt tccttagtag cgtctgcttg
     5161 gtacccagct tcggccgtat ttacagataa cgatgtcgtt attatatata tatatatata
     5221 ttgtcacttg tacccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     5281 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaacagc tgacaccgat
     5341 tatttaaagc tgcagcatac gatatatata catgtgtata tatgtatacc tatgaatgtc
     5401 agtaagtatg tatacgaaca gtatgatact gaagatgaca aggtaatgca tcattctata
     5461 cgtgtcattc tgaacgaggc gcgcctctcc ctgcaggcat taatgaatcg gccaacgcgc
     5521 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5641 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5701 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5761 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5821 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5881 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5941 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6001 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6061 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6301 cagaaaaaaa ggatctcaag aagatccttt gatcttttgt ttaaacctac ggggtctgac
     6361 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6421 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6481 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6541 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6601 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6661 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6721 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6781 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6841 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6901 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tggttgtcag aagtaagttg
     6961 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7021 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7081 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7141 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7201 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7261 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7321 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7381 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7441 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgaaaga
     7501 acgcggcatt agcaacattt gcgaagcgaa cgattttgcg aactgctatt aattaactct
     7561 ggcgcgcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
     7621 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
     7681 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
     7741 caagataaac gaaggcacat atgaggaggg cttttgtaga aagaaatacg aacgaaacga
     7801 aaatcagcgt tgccatcgct ttggacaaag ctcccttacc tgaagagtcg aattttattg
     7861 atgaacttat aacttccaag catgcaaacc aaaagggaga acaagtaatc caagtagaca
     7921 cgggaattgg attcttggat cacatgtatc atgcactggc taaacatgca ggctggagct
     7981 tacgacttta ctcaagaggt gatttaatca tcgatgatca tcacactgca gaagatactg
     8041 ctattgcact tggtattgca ttcaagcagg ctatgggtaa ctttgccggt gttaaaagat
     8101 ttggacatgc ttattgtcca cttgacgaag ctctttccag aagcgtagtt gacttgtcgg
     8161 gacggcccta tgctgttatc gatttgggat taaagcgtga aaaggttggg gaattgtcct
     8221 gtgaaatgat ccctcactta ctatattcct tttcggtagc tgctggaatt actttgcatg
     8281 ttacctgctt atatggtagt aatgaccatc atcgtgctga aagcgctttt aaatctctgg
     8341 ctgttgccat gcgcgcggct acttctctta ctggaagttc tgaagtccca agcacgaagg
     8401 gagtgttgta aagatgaatt ggattatgtc aggaaaagaa cgacaatttt gcatccaaat
     8461 tgtctaaatt ttagagttgc ttgaaaacaa tagaacctta cttgctttat aattacgtta
     8521 attagaagcg ttatctcgtg aaggaatata gtacgtagcc gtataaattg aattgaatgt
     8581 tcagcttata gaatagagac actttgctgt tcaatgcgtc gtcacttacc atactcactt
     8641 tattatacga ctttaagtat aaactccgcg gttatggtaa aattaatgat gcacaaacgt
     8701 ccgatcggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8761 cgttgtaaaa cgacggccag tgaattgtaa tacgactca
//
