LOCUS       sequence-309481-        9772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of HO endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG713_lexO-HO_LexA-TF_URA3MX
FEATURES             Location/Qualifiers
     source          1..9772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(317..336)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(318..336)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(343..360)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(343..359)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(352..374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        462..805
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1624..1821
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        2186..2290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2291..2359,2360..3151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2509..2528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3330..3918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3819..3838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4072..4089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4399..4415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4404..4426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4423..4439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4423..4439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4460..4478
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(4641..4890)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(5354..6244)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(5357..6292)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(join(6311..6664,6665..6916))
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     complement(6352..6373)
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     terminator      complement(7586..7835)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(7885..7908)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(8512..8523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agttttatat acatacagag cacatgctct agaccctggg tgtatataca gttatgctgt
       61 gagtatatac agcacctaga ccctgggtgt atatacagtt atgctgtgag tatatacagc
      121 acctagaccc tgggtgtata tacagttatg ctgtgagtat atacagcacc tagaccctgg
      181 gtgtatatac agttatgctg tgagtatata cagcacctag ggagctccaa ttcgccctat
      241 aggcggccgc gaaaagaaat atagtcttca aggttatatt atacaggttt atatattatt
      301 ctgtttctcc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccgat
      421 cggtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
      481 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
      541 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
      601 cgcgaagcaa aaattacggc tcctcgctgc ggacctgcga gcagggaaac gctcccctca
      661 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
      721 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
      781 acatcacatc cgaacataaa caaccatgac agtcaacact aagacctata gtgagagagc
      841 agaaactcat gcctcaccag tagcacaacg attatttcga ttaatggaac tgaagaaaac
      901 caatttatgt gcatcaattg atgttgatac cactaaggaa tttcttgaat taattgataa
      961 attgggtcct tatgtatgct taatcaagac tcatattgat ataatcaatg atttttccta
     1021 tgaatccact attgaaccat tattagaact ttcacgtaaa catcaattta tgatttttga
     1081 agatagaaaa tttgctgata ttggtaatac cgtgaagaaa caatatattg gtggagttta
     1141 taaaattagt agttgggcag atattactaa tgctcatggt gtcactggga atggagtagt
     1201 tgaaggatta aaacagggag ctaaagaaac caccaccaac caagagccaa gagggttatt
     1261 gatgttagct gaattatcat cagtgggatc attagcttat ggagaatatt ctcaaaaaac
     1321 tgttgaaatt gctaaatccg ataaggaatt tgttattgga tttattgccc aacgtgatat
     1381 gggtggacaa gaagaaggat ttgattggct tattatgaca cctggagttg gattagatga
     1441 taaaggtgat ggattaggac aacaatatag aactgttgat gaagttgtta gcactggaac
     1501 tgatattatc attgttggta gaggattgtt tggtaaagga agagatccag atattgaagg
     1561 taaaaggtat agagatgctg gttggaatgc ttatttgaaa aagactggcc aattataatc
     1621 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     1681 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     1741 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     1801 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa
     1861 acgcatatgc cgggaatctc ggtcgtaatg atttctataa tgacgaaaaa aaaaaaattg
     1921 gaaagaaaaa ccttcatggc ctttataaaa aggaactatc caatacctcg ccagaaccaa
     1981 gtaacagtat tttacggggc acaaatcaag aacaataaga caggactgta aagatggacg
     2041 cattgaactc caaagaacaa caagagttcc aaaaagtagt ggcgcgccag agttaattaa
     2101 tagcagttcg caaaatcgtt cgcttcgcaa atgttgctaa tgccgcgttc tttcgacgtc
     2161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2281 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2341 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2401 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2461 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2521 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2581 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2641 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2701 gacaaccatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2761 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2821 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2881 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2941 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3001 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3061 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3121 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3181 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3241 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3301 aggtttaaac aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3661 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3721 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3781 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3841 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3901 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3961 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4021 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4081 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4141 attcattaat gcctgcaggg agaggcgcgc caattgatga caatacagac gatgataaca
     4201 aaccgaagtt atctgatgta gaaaaggatt aaagatgcta agagatagtg atgatatttc
     4261 ataaataatg taattctata tatgttaatt accttttttg cgaggcatat ttatggtgaa
     4321 ggataagttt tgaccatcaa agaaggttaa tgtggctgtg gtttcagggt ccataaagct
     4381 attcaattca tccagctgtt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4441 atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg ggtacaagtg
     4501 acaatatata tatatatata taataacgac atcgttatct gtaaatacgg ccgaagctgg
     4561 gtaccaagca gacgctacta aggaaaacaa caacggaagc gatgatgaga gcgacgatga
     4621 gagtgtaaac tgcgaacctt gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
     4681 aaggttttca gtataatgtt acatgcgtac acgcgtttgt acagaaaaaa aagaaaaatt
     4741 tgaaatataa ataacgttct taatactaac ataactatta aaaaaaataa atagggacct
     4801 agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtggga ggagggcgtg
     4861 aatgtaagcg tgacataact aattacatga tatagacaaa ggaaaagggg cctgtctcga
     4921 ggataagctt taaacgcgtt taaagcttga aacacaaatc agtaccacca gaatgtcttt
     4981 cagctctaaa ttcaccagag gatcttcttt cagcaactgg atcgaaatca tcagcagatg
     5041 gaatagcttc ttcgaatttt tccaaaccag aatcgaaatt aactttagca tctttaacag
     5101 tcaaagtttc ccaaatcaaa tcagattgtt gttccaaaac accaatagct tcacccaaag
     5161 aagcaacagc agtatccaat ttattaacag aacctggagc atcgaaaacc aattcagcaa
     5221 taccatcttc caaccaatcc aaatacaaag tatcaccttt atacaacata tcagtttctt
     5281 ggattctcaa agtaatacct ggaaattcgc ccgcggtgct gttatcgttg cccggcagat
     5341 cctgcatgat atcgactgtg gcagggaaac cctctgcctc ccccgtgatg taatactttt
     5401 gcaaggaatg cgatgaagta gagcccgcag tggccaagtg gctttggtcc gtctcctcca
     5461 cggatgcccc tccacggcta gtgggcgcat gtaggcggtg ggcgtccagc atctccagca
     5521 gcaggtcata gaggggcacc acgttcttgc acttcatgct gtacagatgc tccatgcctt
     5581 tgttactcat gtgcctgatg tgggagagga tgaggaggag ctgggccagc cgctggtgct
     5641 gctgctgcag ggtcaggcct gccttggcca tcaggtggat caaagtgtct gtgatcttgt
     5701 ccaggactcg gtggatatgg tccttctctt ccagagactt cagggtgctg gacagaaatg
     5761 tgtacactcc agaattaagc aaaataatag atttgaggca cacaaactcc tctccctgca
     5821 gattcatcat gcggaaccga gatgatgtag ccagcagcat gtcgaagatc tccaccatgc
     5881 cctctacaca ttttccctgg ttcctgtcca agagcaagtt aggagcaaac agtagcttca
     5941 ctgggtgctc catggagcgc cagacgagac caatcatcag gatctctaac caggcacatt
     6001 ctagaaggtg gacctgatca tggagggtca aatccacaaa gcctggcacc ctcttcgccc
     6061 agttgatcat gtgaaccagc tccctgtctg ccaggttggt cagtaagccc atcatcgaag
     6121 cttcactgaa gggtctggta ggatcatact cggaatagag tatggggggc tcagcatcca
     6181 acaaggcact gaccatctgg tcggccgtca gggacaaggc caggctgttc ttcttagagc
     6241 gtttgatcat gagcgggctt ggccaaaggt tggcagctct catgtctcca gcagagatat
     6301 ccttagaacc cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
     6361 agctctgctg acgaagatct acgacaattg gtttaaactc gctattttct ggcaacagtt
     6421 cgactttatt gccctgtttt ttcaggcgct taacggtaac ctcgtcatca atacgtgcga
     6481 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcaccatcca
     6541 taatgccgat gtctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
     6601 tgaataagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagtggtt
     6661 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
     6721 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
     6781 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
     6841 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
     6901 ccgttaacgc tttcatgaat tcctgcagcc cgggggatcc actagttgtt aattcagtaa
     6961 attttcgatc ttgggaagaa aaaagcagta agcgtgaaaa atctaaaagc tgatgtagta
     7021 gaagatccta ttctttaaca aagattgacc ttttcttttt cttcttggtt tgagtagaaa
     7081 ggggaaggaa gaatacaaga gagaggaaaa aaaggaagat aaaaagagag cgtgatataa
     7141 atgaatatat attaaacaag agagattggg aaggaaagga tcaaacaaac ccaaaaatat
     7201 ttcaaaaagg agagagagag gcgagtttgg tttcaaaacg gtttatttat ttatgcaaga
     7261 ggacgtggaa gaaaaagaag aaggaagaaa aaaatttgaa agaaaaaaac gcgtggcggg
     7321 taaagaagaa aatggaaaat agaggccggg tgacagagaa atattgaggg ttaattggaa
     7381 aatatgttag ggtgaggcat atgtttttaa gggttttgag gatccgataa ggaagaatgt
     7441 aggttaaatg ttgtgcatta attgctgtgg cagcttaccc gcttctctag agcggccgaa
     7501 gctgggtacc aagcagacgc tactaaggaa aacaacaacg gaagcgatga tgagagcgac
     7561 gatgagagtg taaactgcga accttgcaaa ttaaagcctt cgagcgtccc aaaaccttct
     7621 caagcaaggt tttcagtata atgttacatg cgtacacgcg tttgtacaga aaaaaaagaa
     7681 aaatttgaaa tataaataac gttcttaata ctaacataac tattaaaaaa aataaatagg
     7741 gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatg tgggaggagg
     7801 gcgtgaatgt aagcgtgaca taactaatta catgatatag acaaaggaaa aggggcctgt
     7861 ctcgaggtta gttagttaaa gcttcttgtc gtcgtcatcc ttgtagtcga tatcgcagat
     7921 gcgcgcacct gcgttgttac cacaactctt atgaggcccg cggacagcat caaactgtaa
     7981 gattccgcca cattttatac actctggtcc tttaactggc aaaccttcgg gcgtaatgcc
     8041 caatttttcg cctttgtctt ttgccttttt cacttcacgt gcttctggta catacttgca
     8101 atttatacag tgatgaccgc tgaatttgta tcttccatag catctagcac atactcgatt
     8161 tttaccactc caatctttat aaaaatactt gattcccttt ctgggacaag caacacagtg
     8221 ttttagattc tttttttgtg atattttatg ctgttctccc acacagcagc ctcgacatga
     8281 tttcacttct attttgttgc caagcaagaa atttttatgg ccttctatcg taagcccata
     8341 tacagtactc tcaccctgga aatcatccgt gaagctgaaa tatacgggtt ccctttttat
     8401 aattggcgga acttctcttg ttttgtgacc acttcgacaa tatgacaaaa cattctgtaa
     8461 agttgttccc ccagcaacat tacagtcgta tgtaaattga cattggactt ttcttccttc
     8521 aatgatttcc tccctagctg acctggtcgt cacagtagct gacataccaa gagatcttga
     8581 aatatggaca attccgtcca taatggatga ataaacagtt tgaattgcta ttttataaga
     8641 ttcagggcct tcgccctttt tcacaacgta cccatctgag tcgatcaagc cggctaagaa
     8701 tgcttcacga acttctatat gctcgccgta cataaattca gggatttgct tctctccatc
     8761 aagatccctt ttaaacttta aaattgtgac agctttccag aatggattat ttttcctcaa
     8821 attccttgtc ttcctgtttt catctggacc atctccataa tgaagcctta catgtttggc
     8881 acgtagcgga acgtgatcgt cacaaaccgt aaggtagaga ccccagattt tcgcattttc
     8941 tcttaaactc tccattagct taggatccaa gctatctact gagatttctg gctcttttgt
     9001 tgtaccgtca cctaaccaca gaccaagcat ccaagccata ctttttacag caggagttac
     9061 aaggtcacta cgtccagtga gaaatttaga taaaacacca tttcctgtga gtactggacc
     9121 aaatcttatg cagctagaaa ttctcaattg agcatcaaga taatccaaat ctctaacttc
     9181 aatgtcaaag ttgaaatatt ctcctttaga gcgctccatt tcttctatga agcgttttgc
     9241 ggcaaactca ccttcaactg tcattgggaa tgtcttatga tggttttttg gaattattat
     9301 tatcctacca tcaagcgtct gacattgctg cagatttctc catctcactt tatatttggt
     9361 ggcatttcta ccactttttt ccaacagtgg tttggtaggg accctgactg acaatttatg
     9421 acctgcagta cattgtaatg caagacgctg ataaactgtt ctacgcctgg gatctaacct
     9481 accaggttca ccttcaaaag ctctgtgttt ggttttttgc tgtatattat agattttctg
     9541 atagccctgt gtgacattta tgacgcgggc agcggagcca tctgcgcaca taacgtaaga
     9601 gttagccgtg acgtttgcga tgtctttaat ttcaccgtta gccatcagaa tagtcgtgtt
     9661 ttcagaaagc atgaattcct gcagcccggg ggatccacta gtatagaagt atagtaattt
     9721 atgctgcaaa ggtcctaatg tataaggaaa gaagagaaaa gaagaaaaca ag
//
