LOCUS       sequence-309494-         369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Flower TG, Buffalo CZ, Hooy RM, Allaire M, Ren X, Hurley JH
  TITLE     Structure of SARS-CoV-2 ORF8, a rapidly evolving immune evasion 
            protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2021785118. doi: 
            10.1073/pnas.2021785118.
  PUBMED    33361333
REFERENCE   2  (bases 1 to 369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-12"; volume: "118(2):e2021785118. 
            doi"; pages: " 10.1073/pnas.2021785118"
COMMENT     Sequence Label: V32M,L84M CoV-2 ORF8
FEATURES             Location/Qualifiers
     source          1..369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgaaatctt ctcaccatca ccatcaccat gaaaacctgt acttccaatc caatgcacaa
       61 gaatgctctt tgcagtcttg tacacagcat caaccttata tggtcgatga tccttgcccg
      121 attcactttt acagtaaatg gtatatcagg gtaggggccc gaaaaagcgc accattgatt
      181 gaattgtgtg tggacgaggc agggagcaaa agtccgattc aatacattga catcggtaat
      241 tatactgtca gttgtatgcc ctttacgatc aactgccaag aacccaaact ggggagcttg
      301 gtggtccgat gttccttcta tgaagatttt cttgagtatc acgatgtcag ggtggtcctt
      361 gactttata
//
