LOCUS       sequence-309499-        8247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-SARS-CoV-2 NSP2
FEATURES             Location/Qualifiers
     source          1..8247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2750..2774)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2821..2844
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2881..2904
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2935..2961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2935..2961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2962..3309,3310..3312,3313..3921)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(3965..3982)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3971..4195
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4328..4347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4479..4526
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4534..5556
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4534..5556
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5686..5807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5723..5742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5777..5796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5856..5872)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5856..5872)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5869..5891)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5880..5896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5904..5910,5911..5928,5929..5934))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5949..5970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6087..6104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6258..6846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6338..6357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7017..7808,7809..7877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7640..7659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7878..7982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8057..8076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac catggcctac
      841 acgcgatatg ttgacaacaa cttttgtgga cctgacggct accctcttga atgtattaag
      901 gatcttctgg ctcgcgctgg aaaagccagt tgtacactgt ctgagcagct cgactttata
      961 gatacaaagc ggggggtcta ttgttgccga gagcatgaac atgagatcgc atggtatacg
     1021 gagcgctctg aaaaaagcta tgaattgcag acaccctttg aaattaagtt ggcgaaaaag
     1081 ttcgatacat tcaatggtga gtgccccaac tttgtctttc cccttaacag tatcattaaa
     1141 acaatccagc cccgggtgga aaagaagaaa ttggatggat tcatgggcag gatcagaagt
     1201 gtttacccag tggcatcccc taatgaatgc aatcagatgt gcctgtccac gctgatgaaa
     1261 tgcgaccact gcggtgaaac gtcttggcaa acgggggact ttgtaaaagc aacgtgcgag
     1321 ttttgcggaa ctgagaactt gacaaaggag ggagcgacta catgtggata cctgcctcag
     1381 aacgcggtag tgaaaatata ctgtcctgcc tgtcacaatt ctgaagttgg gcctgaacat
     1441 agcttggccg aataccataa cgaaagtgga ttgaagacaa ttcttcgcaa aggaggccgg
     1501 actatcgcgt tcgggggctg cgtgtttagc tatgtaggat gtcataataa atgcgcatat
     1561 tgggttccac gagctagcgc aaacataggc tgcaaccaca caggcgtagt tggtgaggga
     1621 tcagaaggtc ttaatgataa cttgctcgaa atattgcaaa aggaaaaagt caacataaat
     1681 attgtaggag attttaaatt gaatgaagag attgcgataa tccttgcgtc attttccgca
     1741 agtacgagtg cgttcgttga gactgtaaag gggctcgact ataaagcgtt taagcaaata
     1801 gtagagagct gcggaaactt taaagttacg aagggaaaag ccaaaaaagg tgcatggaac
     1861 attggcgagc agaagagtat actgtccccc ctgtacgcct tcgcgtcaga agcagctcga
     1921 gtagttagaa gtatattctc acgaacgctc gagacggcgc agaatagtgt gcgcgtgctc
     1981 cagaaggctg ctattacgat cctcgacggc atatcccagt attctcttcg actgattgat
     2041 gctatgatgt tcacctccga cttggcgacg aacaatctcg tagttatggc ttatatcacc
     2101 ggcggcgttg tacaattgac gtctcagtgg ctgacgaaca tcttcggaac cgtgtacgag
     2161 aagctcaaac cagttcttga ttggcttgaa gaaaagttta aggaaggcgt cgagtttctc
     2221 cgggatgggt gggaaattgt taagtttatc tccacctgcg cgtgtgaaat tgtgggaggg
     2281 caaattgtca cctgcgccaa ggaaattaaa gagtcagttc agacgttttt taaacttgtg
     2341 aacaaattcc ttgctctctg tgcggatagt ataatcatcg gtggggcgaa gttgaaagca
     2401 ctcaatcttg gtgaaacttt cgtgacccac agcaaaggcc tgtataggaa gtgtgttaaa
     2461 agcagggagg agaccggtct tcttatgccg ctgaaggcgc ccaaagagat aatttttctt
     2521 gagggggaaa ctcttccgac cgaagtgctc acggaagaag tagttctgaa aacaggggac
     2581 cttcaaccac tggagcaacc gacctctgag gcagttgaag cgccgctcgt cggaactcct
     2641 gtatgtatta acggcctcat gctgttggaa attaaggata cggagaagta ctgtgcactg
     2701 gcccccaaca tgatggtcac gaacaacacc tttaccctta agggcggcga cccagctttc
     2761 ttgtacaaag tggttgatat caaggccgcc gacatcacca gcctgtacaa gaaagtgggg
     2821 tggagccatc cccagttcga gaagggcgga ggatctggcg gcggaagcgg cggaggaagc
     2881 tggtcccacc ctcagtttga gaaaggcacc gagctgggca gcaccatggc cagctacccc
     2941 tacgacgtgc ccgactacgc caaggacaac accgtgcccc tgaagctgat cgccctgctg
     3001 gccaacggcg agttccactc tggcgagcag ctgggcgaga cactgggcat gagcagagcc
     3061 gccatcaaca agcacatcca gaccctgcgg gactggggcg tggacgtgtt cacagtgccc
     3121 ggcaagggct acagcctgcc cgagcccatc cagctgctga acgccaagca gatcctgggc
     3181 cagctggatg gcggatctgt ggccgtgctg cccgtgatcg acagcaccaa ccagtatctg
     3241 ctcgaccgga tcggcgagct gaagtccggc gacgcctgta tcgccgagta ccagcaggcc
     3301 ggcagaggcg gcaggggcag aaagtggttc agccccttcg gcgccaacct gtacctgagc
     3361 atgttctgga gactggaaca gggcccagcc gccgctattg gcctgagcct ggtcatcggc
     3421 atcgtgatgg ccgaggtgct gcggaagctg ggcgccgaca aagtgcgcgt gaagtggccc
     3481 aacgacctgt atctgcagga ccggaagctg gccggcatcc tggtggaact gaccggcaaa
     3541 acaggcgacg ccgcccagat cgtgatcggc gctggcatca acatggccat gcggcgggtg
     3601 gaagagagcg tggtcaacca gggctggatc accctgcagg aagccggcat caacctggac
     3661 cggaacaccc tggccgccat gctgatcaga gagctgagag ccgccctgga actgttcgag
     3721 caggaaggcc tggcccccta cctgagcaga tgggagaagc tggacaactt catcaaccgg
     3781 cccgtgaaac tgatcatcgg cgacaaagag atcttcggca tcagccgggg catcgacaag
     3841 cagggggccc tgctcctgga acaggacggc atcatcaagc cctggatggg cggcgagatc
     3901 agcctgcgga gcgccgagaa gtgatgactc gagtctagag ggcccgttta aacccgctga
     3961 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4021 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4081 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4141 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4201 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     4261 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4321 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4381 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4441 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
     4501 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
     4561 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     4621 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     4681 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     4741 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     4801 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     4861 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     4921 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     4981 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     5041 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     5101 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     5161 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     5221 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     5281 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     5341 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     5401 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     5461 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     5521 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     5581 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5641 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     5821 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5881 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5941 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6001 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6061 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7981 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     8041 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8101 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8161 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8221 gcgatgtacg ggccagatat acgcgtt
//
