LOCUS       sequence-30950-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI
  TITLE     HES1 inhibits cycling of hematopoietic progenitor cells via DNA 
            binding.
  JOURNAL   Stem Cells. 2006 Apr . 24(4):876-88.
  PUBMED    16513761
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2006 Apr . 24(4):876-88."
COMMENT     Sequence Label: EF.deltaBHES1.Ubc.GFP
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc caagctggct
       61 aggtaagctt gatatcgaat tcgcccacca tggactacaa agacgatgac gacaagccag
      121 ctgatataat ggagaaaaat tcctcgtccc cggtggctgc taccccagcc agtgtcaaca
      181 cgacaccgga taaaccaaag acagcatctg agcacagaaa gtcatcaaag cctattatgg
      241 cggcgagacg agcggcaaga ataaatgaaa gtctgagcca gctgaaaaca ctgattttgg
      301 atgctctgaa gaaagatagc tcgcggcatt ccaagctgga gaaggcggac attctggaaa
      361 tgacagtgaa gcacctccgg aacctgcagc gggcgcagat gacggctgcg ctgagcacag
      421 acccaagtgt gctggggaag taccgagccg gcttcagcga gtgcatgaac gaggtgaccc
      481 gcttcctgtc cacgtgcgag ggcgttaata ccgaggtgcg cactcggctg ctcggccacc
      541 tggccaactg catgacccag atcaatgcca tgacctaccc cgggcagccg caccccgcct
      601 tgaaggtgcc gcctccggca ccactgggga cccggcggcc tcc
//
