LOCUS       sequence-309500-        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-SARS-CoV-2 NSP12
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3584..3608)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3655..3678
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3715..3738
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3769..3795
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3769..3795
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3796..4143,4144..4146,4147..4755)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4799..4816)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4805..5029
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5162..5181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5313..5360
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5368..6390
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5368..6390
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6520..6641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6557..6576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6611..6630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6690..6706)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6690..6706)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6703..6725)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6714..6730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6738..6744,6745..6762,6763..6768))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6783..6804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6921..6938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7092..7680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7172..7191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7851..8642,8643..8711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8474..8493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8712..8816)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8891..8910)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatgtc cgccgctaga
      841 ctgactcctt gtggtactgg cacctctact gacgtggtgt acagggcctt cgacatctac
      901 aacgacaagg tggccggatt cgctaagttc ctgaagacca actgctgccg cttccaggag
      961 aaggacgaag acgacaacct gatcgactct tacttcgtgg tgaagaggca caccttcagc
     1021 aactaccagc acgaggaaac catctacaac ctgctgaagg actgccccgc cgtggctaag
     1081 cacgacttct tcaagttccg catcgacggt gacatggtgc ctcacatcag ccgccagagg
     1141 ctgaccaagt acaccatggc cgacctggtg tacgctctga ggcacttcga cgagggcaac
     1201 tgcgacaccc tgaaggaaat cctggtgacc tacaactgct gcgacgacga ctacttcaac
     1261 aagaaggact ggtacgactt cgtggagaac cctgacatcc tgagagtgta cgccaacctg
     1321 ggcgaaagag tgcgtcaggc tctgctgaag accgtgcagt tctgcgacgc catgcgtaac
     1381 gctggtatcg tgggcgtgct gaccctggac aaccaggacc tgaacggcaa ctggtacgac
     1441 ttcggagact tcatccagac tactccagga tctggtgtgc ctgtggtgga ctcctactac
     1501 tctctgctga tgccaatcct gaccctgacc agggccctga ccgctgagtc tcacgtggac
     1561 accgacctga ccaagcccta catcaagtgg gacctgctga agtacgactt caccgaggaa
     1621 cgcctgaagc tgttcgacag gtacttcaag tactgggacc agacctacca cccaaactgc
     1681 gtgaactgcc tggacgacag atgcatcctg cactgcgcca acttcaacgt gctgttctct
     1741 accgtgttcc ctcccaccag cttcggccct ctggtgcgta agatcttcgt ggacggcgtg
     1801 ccattcgtgg tgtctaccgg ataccacttc agagagctgg gtgtggtgca caaccaggac
     1861 gtgaacctgc acagctcccg tctgagcttc aaggaactgc tggtgtacgc tgctgaccct
     1921 gctatgcacg ctgctagcgg caacctgctg ctggacaagc gcaccacctg cttctccgtg
     1981 gccgctctga ccaacaacgt ggccttccag accgtgaagc ctggaaactt caacaaggac
     2041 ttctacgact tcgctgtgag caagggattc ttcaaggagg gttctagcgt ggaactgaag
     2101 cacttcttct tcgcccagga cggcaacgcc gctatctccg actacgacta ctaccgctac
     2161 aacctgccca ccatgtgcga catcaggcag ctgctgttcg tggtggaggt ggtggacaag
     2221 tacttcgact gctacgacgg cggatgcatc aacgctaacc aggtcatcgt gaacaacctg
     2281 gacaagagcg ccggcttccc tttcaacaag tggggaaagg ctagactgta ctacgacagc
     2341 atgtcctacg aagaccagga cgccctgttc gcttacacca agcgtaacgt gatcccaacc
     2401 atcacccaga tgaacctgaa gtacgccatc tccgctaaga accgcgccag gaccgtggct
     2461 ggtgtgagca tctgctccac catgaccaac agacagttcc accagaagct gctgaagtct
     2521 atcgccgcta cccgtggtgc caccgtggtc atcggcacca gcaagttcta cggtggctgg
     2581 cacaacatgc tgaagaccgt gtactccgac gtggagaacc cacacctgat gggctgggac
     2641 taccccaagt gcgacagagc tatgcctaac atgctgcgta tcatggcctc tctggtgctg
     2701 gctagaaagc acaccacctg ctgctctctg agccaccgct tctacaggct ggccaacgag
     2761 tgcgctcagg tgctgtccga aatggtcatg tgcggaggtt ctctgtacgt gaagcccggc
     2821 ggaacctcct ctggagacgc taccaccgcc tacgctaact ccgtgttcaa catctgccag
     2881 gccgtgaccg ctaacgtgaa cgccctgctg tctaccgacg gtaacaagat cgctgacaag
     2941 tacgtgagaa acctgcagca ccgtctgtac gagtgcctgt acagaaaccg tgacgtggac
     3001 accgacttcg tgaacgaatt ctacgcctac ctgcgcaagc acttctccat gatgatcctg
     3061 tctgacgacg ctgtggtgtg cttcaactct acctacgcca gccagggcct ggtggctagc
     3121 atcaagaact tcaagtccgt gctgtactac cagaacaacg tgttcatgag cgaggccaag
     3181 tgctggaccg aaaccgacct gaccaaggga cctcacgagt tctgctccca gcacaccatg
     3241 ctggtgaagc agggtgacga ctacgtgtac ctgccatacc ctgacccttc tagaatcctg
     3301 ggagctggat gcttcgtgga cgacatcgtg aagaccgacg gcaccctgat gatcgaaagg
     3361 ttcgtgagcc tggccatcga cgcttaccct ctgaccaagc acccaaacca ggagtacgct
     3421 gacgtgttcc acctgtacct gcagtacatc cgcaagctgc acgacgaact gaccggacac
     3481 atgctggaca tgtacagcgt gatgctgacc aacgacaaca cctccaggta ctgggagcca
     3541 gaattctacg aggccatgta cacccctcac accgtgctgc agtacccaac tttcttgtac
     3601 aaagtggttg atatcaaggc cgccgacatc accagcctgt acaagaaagt ggggtggagc
     3661 catccccagt tcgagaaggg cggaggatct ggcggcggaa gcggcggagg aagctggtcc
     3721 caccctcagt ttgagaaagg caccgagctg ggcagcacca tggccagcta cccctacgac
     3781 gtgcccgact acgccaagga caacaccgtg cccctgaagc tgatcgccct gctggccaac
     3841 ggcgagttcc actctggcga gcagctgggc gagacactgg gcatgagcag agccgccatc
     3901 aacaagcaca tccagaccct gcgggactgg ggcgtggacg tgttcacagt gcccggcaag
     3961 ggctacagcc tgcccgagcc catccagctg ctgaacgcca agcagatcct gggccagctg
     4021 gatggcggat ctgtggccgt gctgcccgtg atcgacagca ccaaccagta tctgctcgac
     4081 cggatcggcg agctgaagtc cggcgacgcc tgtatcgccg agtaccagca ggccggcaga
     4141 ggcggcaggg gcagaaagtg gttcagcccc ttcggcgcca acctgtacct gagcatgttc
     4201 tggagactgg aacagggccc agccgccgct attggcctga gcctggtcat cggcatcgtg
     4261 atggccgagg tgctgcggaa gctgggcgcc gacaaagtgc gcgtgaagtg gcccaacgac
     4321 ctgtatctgc aggaccggaa gctggccggc atcctggtgg aactgaccgg caaaacaggc
     4381 gacgccgccc agatcgtgat cggcgctggc atcaacatgg ccatgcggcg ggtggaagag
     4441 agcgtggtca accagggctg gatcaccctg caggaagccg gcatcaacct ggaccggaac
     4501 accctggccg ccatgctgat cagagagctg agagccgccc tggaactgtt cgagcaggaa
     4561 ggcctggccc cctacctgag cagatgggag aagctggaca acttcatcaa ccggcccgtg
     4621 aaactgatca tcggcgacaa agagatcttc ggcatcagcc ggggcatcga caagcagggg
     4681 gccctgctcc tggaacagga cggcatcatc aagccctgga tgggcggcga gatcagcctg
     4741 cggagcgccg agaagtgatg actcgagtct agagggcccg tttaaacccg ctgatcagcc
     4801 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4861 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4921 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4981 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5041 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5281 aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct
     5341 ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag
     5401 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     5461 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     5521 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     5581 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     5641 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     5701 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     5761 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     5821 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     5881 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     5941 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     6001 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     6061 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     6121 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     6181 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     6241 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     6301 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     6361 cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc
     6421 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6481 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6541 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6601 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6661 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6721 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6781 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8821 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     8881 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     8941 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9001 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9061 tacgggccag atatacgcgt t
//
