LOCUS       sequence-309501-       12162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-SARS-CoV-2 NSP3
FEATURES             Location/Qualifiers
     source          1..12162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6665..6689)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6736..6759
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6796..6819
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6850..6876
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6850..6876
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6877..7224,7225..7227,7228..7836)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7880..7897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7886..8110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8243..8262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8394..8441
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8449..9471
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8449..9471
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9601..9722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9638..9657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9692..9711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9771..9787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9771..9787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9784..9806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9795..9811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9819..9825,9826..9843,9844..9849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9864..9885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10002..10019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10173..10761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10253..10272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10932..11723,11724..11792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11555..11574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11793..11897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11972..11991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatggc ccctaccaag
      841 gtgaccttcg gagacgacac cgtgatcgag gtgcagggtt acaagagcgt gaacatcacc
      901 ttcgagctgg acgaacgcat cgacaaggtg ctgaacgaaa agtgctccgc ttacaccgtg
      961 gagctgggca ccgaggtgaa cgaattcgcc tgcgtggtgg ccgacgctgt gatcaagacc
     1021 ctgcagccag tgtccgaact gctgacccct ctgggtatcg acctggacga gtggtctatg
     1081 gctacctact acctgttcga cgagagcggc gaattcaagc tggcctctca catgtactgc
     1141 agcttctacc ctcccgacga agacgaggaa gagggtgact gcgaagagga agagttcgag
     1201 ccttccactc agtacgagta cggcaccgaa gacgactacc agggcaagcc tctggaattc
     1261 ggagccacct ctgccgctct gcagcctgaa gaggaacagg aggaagactg gctggacgac
     1321 gactcccagc agaccgtggg tcagcaggac ggctctgaag acaaccagac caccaccatc
     1381 cagaccatcg tggaggtgca gcctcagctg gagatggaac tgaccccagt ggtgcagacc
     1441 atcgaggtga acagcttctc cggctacctg aagctgaccg acaacgtgta catcaagaac
     1501 gctgacatcg tggaggaagc caagaaggtg aaaccaaccg tggtggtgaa cgccgctaac
     1561 gtgtacctga agcacggcgg aggtgtggct ggagctctga acaaggctac caacaacgcc
     1621 atgcaggtgg aatccgacga ctacatcgct accaacggtc ctctgaaggt gggcggatcc
     1681 tgcgtgctgt ctggccacaa cctggccaag cactgcctgc acgtggtggg accaaacgtg
     1741 aacaagggag aggacatcca gctgctgaag agcgcctacg aaaacttcaa ccagcacgag
     1801 gtgctgctgg ctccactgct gtctgccgga atcttcggtg ctgatcccat ccacagcctg
     1861 cgcgtgtgcg tggacaccgt gaggaccaac gtgtacctgg ccgtgttcga caagaacctg
     1921 tacgacaagc tggtgagctc cttcctggag atgaagtccg aaaagcaggt ggagcagaag
     1981 atcgccgaga tccccaagga ggaagtgaag cctttcatca ccgaatctaa gcccagcgtg
     2041 gagcagagaa agcaggacga caagaagatc aaggcctgcg tggaggaagt gaccaccacc
     2101 ctggaggaaa ccaagttcct gaccgagaac ctgctgctgt acatcgacat caacggcaac
     2161 ctgcaccctg actccgctac cctggtgtct gacatcgaca tcaccttcct gaagaaggac
     2221 gccccctaca tcgtgggcga cgtggtgcag gaaggagtgc tgaccgctgt ggtcatccct
     2281 accaagaagg ccggtggcac caccgagatg ctggccaagg ctctgcgtaa ggtgccaacc
     2341 gacaactaca tcaccaccta ccccggtcag ggcctgaacg gttacaccgt ggaggaagct
     2401 aagaccgtgc tgaagaagtg caagtctgcc ttctacatcc tgccctccat catctctaac
     2461 gagaagcagg aaatcctggg caccgtgagc tggaacctgc gcgaaatgct ggcccacgct
     2521 gaggaaacca ggaagctgat gcccgtgtgc gtggagacca aggccatcgt gagcaccatc
     2581 cagagaaagt acaagggcat caagatccag gagggtgtgg tggactacgg cgctcgtttc
     2641 tacttctaca cctccaagac caccgtggcc tctctgatca acaccctgaa cgacctgaac
     2701 gagaccctgg tgaccatgcc actgggatac gtgacccacg gtctgaacct ggaggaagcc
     2761 gctagataca tgcgttccct gaaggtgcct gctaccgtga gcgtgagcag ccctgacgct
     2821 gtgaccgcct acaacggata cctgacctcc tctagcaaga cccctgagga acacttcatc
     2881 gaaaccatca gcctggccgg ttcctacaag gactggtctt acagcggaca gtccacccag
     2941 ctgggcatcg agttcctgaa gcgcggagac aagagcgtgt actacacctc caacccaacc
     3001 accttccacc tggacggtga agtgatcacc ttcgacaacc tgaagaccct gctgtctctg
     3061 cgcgaggtga ggaccatcaa ggtgttcacc accgtggaca acatcaacct gcacacccag
     3121 gtggtggaca tgagcatgac ctacggacag cagttcggtc ctacctacct ggacggagct
     3181 gacgtgacca agatcaagcc acacaactcc cacgagggca agaccttcta cgtgctgcct
     3241 aacgatgaca ccctgagagt ggaggctttc gaatactacc acaccaccga cccatccttc
     3301 ctgggcaggt acatgtctgc cctgaaccac accaagaagt ggaagtaccc ccaggtgaac
     3361 ggactgacca gcatcaagtg ggccgacaac aactgctacc tggccaccgc tctgctgacc
     3421 ctgcagcaga tcgaactgaa gttcaaccca cccgctctgc aggacgctta ctacagagcc
     3481 cgtgctggcg aggccgctaa cttctgcgct ctgatcctgg cctactgcaa caagaccgtg
     3541 ggcgaactgg gagacgtgag agagaccatg agctacctgt tccagcacgc caacctggac
     3601 tcctgcaagc gtgtgctgaa cgtggtgtgc aagacctgcg gccagcagca gaccaccctg
     3661 aagggagtgg aagctgtgat gtacatgggt accctgtcct acgagcagtt caagaagggc
     3721 gtgcagatcc cttgcacctg cggaaagcag gccaccaagt acctggtgca gcaggaaagc
     3781 ccattcgtga tgatgtccgc tcctccagcc cagtacgaac tgaagcacgg caccttcacc
     3841 tgcgcttccg agtacaccgg caactaccag tgcggacact acaagcacat cacctctaag
     3901 gagaccctgt actgcatcga cggagccctg ctgaccaagt cctctgaata caagggtccc
     3961 atcaccgacg tgttctacaa ggagaacagc tacaccacca ccatcaagcc cgtgacctac
     4021 aagctggacg gcgtggtgtg caccgaaatc gaccctaagc tggacaacta ctacaagaag
     4081 gacaactctt acttcaccga gcagcctatc gacctggtgc caaaccagcc ctaccctaac
     4141 gccagcttcg acaacttcaa gttcgtgtgc gacaacatca agttcgctga cgacctgaac
     4201 cagctgaccg gatacaagaa gcctgcctct agagagctga aggtgacctt cttcccagac
     4261 ctgaacggcg acgtggtggc tatcgactac aagcactaca ccccaagctt caagaaggga
     4321 gccaagctgc tgcacaagcc catcgtgtgg cacgtgaaca acgctaccaa caaggccacc
     4381 tacaagccta acacctggtg catccgttgc ctgtggtcta ccaagccagt ggaaacctcc
     4441 aactctttcg acgtgctgaa gagcgaggac gctcagggca tggacaacct ggcctgcgaa
     4501 gacctgaagc ccgtgagcga ggaagtggtg gagaacccaa ccatccagaa ggacgtgctg
     4561 gaatgcaacg tgaagaccac cgaggtggtg ggagacatca tcctgaagcc agctaacaac
     4621 agcctgaaga tcaccgagga agtgggtcac accgacctga tggccgctta cgtggacaac
     4681 agctccctga ccatcaagaa gcccaacgag ctgagcaggg tgctgggtct gaagaccctg
     4741 gctactcacg gtctggctgc tgtgaactct gtgccatggg acaccatcgc taactacgcc
     4801 aagcctttcc tgaacaaggt ggtgtccacc accaccaaca tcgtgaccag atgtctgaac
     4861 cgcgtgtgca ccaactacat gccctacttc ttcaccctgc tgctgcagct gtgcaccttc
     4921 accagaagca ccaactcccg tatcaaggct agcatgccta ccaccatcgc caagaacacc
     4981 gtgaagagcg tgggaaagtt ctgcctggag gcttccttca actacctgaa gtctccaaac
     5041 ttcagcaagc tgatcaacat catcatctgg ttcctgctgc tctccgtgtg cctgggttcc
     5101 ctgatctact ctaccgccgc tctgggagtg ctgatgtcta acctgggtat gcctagctac
     5161 tgcactggtt acagagaggg atacctgaac agcaccaacg tgaccatcgc cacctactgc
     5221 accggcagca tcccatgctc cgtgtgcctg tctggactgg acagcctgga cacctacccc
     5281 tccctggaaa ccatccagat caccatctct agcttcaagt gggacctgac cgccttcggt
     5341 ctggtggctg agtggttcct ggcctacatc ctgttcacca ggttcttcta cgtgctgggc
     5401 ctggccgcta tcatgcagct gttcttctcc tacttcgctg tgcacttcat cagcaactcc
     5461 tggctgatgt ggctgatcat caacctggtg cagatggctc ccatctctgc catggtgcgc
     5521 atgtacatct tcttcgccag cttctactac gtgtggaagt cctacgtgca cgtggtggac
     5581 ggttgcaact cctctacctg catgatgtgc tacaagcgca acagggctac cagggtggag
     5641 tgcaccacca tcgtgaacgg cgtgcgcagg tccttctacg tgtacgccaa cggcggcaag
     5701 ggattctgca agctgcacaa ctggaactgc gtgaactgcg acaccttctg cgctggctcc
     5761 accttcatct ctgacgaggt ggccagagac ctgagcctgc agttcaagcg tccaatcaac
     5821 cccaccgacc agagctccta catcgtggac tctgtgaccg tgaagaacgg tagcatccac
     5881 ctgtacttcg acaaggctgg ccagaagacc tacgagcgcc actctctgag ccacttcgtg
     5941 aacctggaca acctgagggc caacaacacc aagggcagcc tgcctatcaa cgtgatcgtg
     6001 ttcgacggaa agtccaagtg cgaggaatct agcgccaagt ccgccagcgt gtactacagc
     6061 cagctgatgt gccagccaat cctgctgctg gaccaggctc tggtgtctga cgtgggagac
     6121 agcgccgaag tggccgtgaa gatgttcgac gcctacgtga acaccttctc ctctaccttc
     6181 aacgtgccta tggagaagct gaagaccctg gtggctactg ctgaggctga actggctaag
     6241 aacgtgtccc tggacaacgt gctgagcacc ttcatctccg ccgctaggca gggtttcgtg
     6301 gactctgacg tggaaaccaa agacgtggtg gagtgcctga agctgtctca ccagagcgac
     6361 atcgaagtga ccggcgactc ctgcaacaac tacatgctga cctacaacaa ggtggagaac
     6421 atgaccccaa gagacctggg agcttgcatc gactgctccg cccgtcacat caacgctcag
     6481 gtggccaagt ctcacaacat cgccctgatc tggaacgtga aggacttcat gagcctgtcc
     6541 gagcagctgc gcaagcagat caggagcgcc gctaagaaga acaacctgcc cttcaagctg
     6601 acctgcgcta ccaccagaca ggtggtgaac gtggtgacca ccaagatcgc cctgaagggc
     6661 ggatacccaa ctttcttgta caaagtggtt gatatcaagg ccgccgacat caccagcctg
     6721 tacaagaaag tggggtggag ccatccccag ttcgagaagg gcggaggatc tggcggcgga
     6781 agcggcggag gaagctggtc ccaccctcag tttgagaaag gcaccgagct gggcagcacc
     6841 atggccagct acccctacga cgtgcccgac tacgccaagg acaacaccgt gcccctgaag
     6901 ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg cgagacactg
     6961 ggcatgagca gagccgccat caacaagcac atccagaccc tgcgggactg gggcgtggac
     7021 gtgttcacag tgcccggcaa gggctacagc ctgcccgagc ccatccagct gctgaacgcc
     7081 aagcagatcc tgggccagct ggatggcgga tctgtggccg tgctgcccgt gatcgacagc
     7141 accaaccagt atctgctcga ccggatcggc gagctgaagt ccggcgacgc ctgtatcgcc
     7201 gagtaccagc aggccggcag aggcggcagg ggcagaaagt ggttcagccc cttcggcgcc
     7261 aacctgtacc tgagcatgtt ctggagactg gaacagggcc cagccgccgc tattggcctg
     7321 agcctggtca tcggcatcgt gatggccgag gtgctgcgga agctgggcgc cgacaaagtg
     7381 cgcgtgaagt ggcccaacga cctgtatctg caggaccgga agctggccgg catcctggtg
     7441 gaactgaccg gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg catcaacatg
     7501 gccatgcggc gggtggaaga gagcgtggtc aaccagggct ggatcaccct gcaggaagcc
     7561 ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct gagagccgcc
     7621 ctggaactgt tcgagcagga aggcctggcc ccctacctga gcagatggga gaagctggac
     7681 aacttcatca accggcccgt gaaactgatc atcggcgaca aagagatctt cggcatcagc
     7741 cggggcatcg acaagcaggg ggccctgctc ctggaacagg acggcatcat caagccctgg
     7801 atgggcggcg agatcagcct gcggagcgcc gagaagtgat gactcgagtc tagagggccc
     7861 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7921 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7981 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8041 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8101 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     8161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc
     8401 ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa
     8461 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     8521 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     8581 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     8641 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     8701 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     8761 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     8821 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     8881 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     8941 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     9001 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     9061 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     9121 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     9181 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     9241 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     9301 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     9361 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     9421 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta
     9481 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9541 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     9601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9721 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    11941 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    12001 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
    12061 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
    12121 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
