LOCUS       sequence-309503-        8709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-FURIN
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    422..446
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1746..1755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2832..2856)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2903..2926
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2963..2986
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3017..3043
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3017..3043
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3044..3391,3392..3394,3395..4003)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4047..4064)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4053..4277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4410..4429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4561..4608
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4616..5638
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4616..5638
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5768..5889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5805..5824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5859..5878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5938..5954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5938..5954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5951..5973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5962..5978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5986..5992,5993..6010,6011..6016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6031..6052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6169..6186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6340..6928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6420..6439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7099..7890,7891..7959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7722..7741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7960..8064)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8139..8158)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8330..8709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
      421 aacaagtttg tacaaaaaag ttggcaccat ggagctgagg ccctggttgc tatgggtggt
      481 agcagcaaca ggaaccttgg tcctgctagc agctgatgct cagggccaga aggtcttcac
      541 caacacgtgg gctgtgcgca tccctggagg cccagcggtg gccaacagtg tggcacggaa
      601 gcatgggttc ctcaacctgg gccagatctt cggggactat taccacttct ggcatcgagg
      661 agtgacgaag cggtccctgt cgcctcaccg cccgcggcac agccggctgc agagggagcc
      721 tcaagtacag tggctggaac agcaggtggc aaagcgacgg actaaacggg acgtgtacca
      781 ggagcccaca gaccccaagt ttcctcagca gtggtacctg tctggtgtca ctcagcggga
      841 cctgaatgtg aaggcggcct gggcgcaggg ctacacaggg cacggcattg tggtctccat
      901 tctggacgat ggcatcgaga agaaccaccc ggacttggca ggcaattatg atcctggggc
      961 cagttttgat gtcaatgacc aggaccctga cccccagcct cggtacacac agatgaatga
     1021 caacaggcac ggcacacggt gtgcggggga agtggctgcg gtggccaaca acggtgtctg
     1081 tggtgtaggt gtggcctaca acgcccgcat tggaggggtg cgcatgctgg atggcgaggt
     1141 gacagatgcg gtggaggcac gctcgctggg cctgaacccc aaccacatcc acatctacag
     1201 tgccagctgg ggccccgagg atgacggcaa gacagtggat gggccagccc gcctcgccga
     1261 ggaggccttc ttccgtgggg ttagccaggg ccgagggggg ctgggctcca tctttgtctg
     1321 ggcctcgggg aacgggggcc gggaacatga cagctgcaac tgcgacggct acaccaacag
     1381 tatctacacg ctgtccatca gcagcgccac gcagtttggc aacgtgccgt ggtacagcga
     1441 ggcctgctcg tccacactgg ccacgaccta cagcagtggc aaccagaatg agaagcagat
     1501 cgtgacgact gacttgcggc agaagtgcac ggagtctcac acgggcacct cagcctctgc
     1561 ccccttagca gccggcatca ttgctctcac cctggaggcc aataagaacc tcacatggcg
     1621 ggacatgcaa cacctggtgg tacagacctc gaagccagcc cacctcaatg ccaacgactg
     1681 ggccaccaat ggtgtgggcc ggaaagtgag ccactcatat ggctacgggc ttttggacgc
     1741 aggcgccatg gtggccctgg cccagaattg gaccacagtg gccccccagc ggaagtgcat
     1801 catcgacatc ctcaccgagc ccaaagacat cgggaaacgg ctcgaggtgc ggaagaccgt
     1861 gaccgcgtgc ctgggcgagc ccaaccacat cactcggctg gagcacgctc aggcgcggct
     1921 caccctgtcc tataatcgcc gtggcgacct ggccatccac ctggtcagcc ccatgggcac
     1981 ccgctccacc ctgctggcag ccaggccaca tgactactcc gcagatgggt ttaatgactg
     2041 ggccttcatg acaactcatt cctgggatga ggatccctct ggcgagtggg tcctagagat
     2101 tgaaaacacc agcgaagcca acaactatgg gacgctgacc aagttcaccc tcgtactcta
     2161 tggcaccgcc cctgaggggc tgcccgtacc tccagaaagc agtggctgca agaccctcac
     2221 gtccagtcag gcctgtgtgg tgtgcgagga aggcttctcc ctgcaccaga agagctgtgt
     2281 ccagcactgc cctccaggct tcgcccccca agtcctcgat acgcactata gcaccgagaa
     2341 tgacgtggag accatccggg ccagcgtctg cgccccctgc cacgcctcat gtgccacatg
     2401 ccaggggccg gccctgacag actgcctcag ctgccccagc cacgcctcct tggaccctgt
     2461 ggagcagact tgctcccggc aaagccagag cagccgagag tccccgccac agcagcagcc
     2521 acctcggctg cccccggagg tggaggcggg gcaacggctg cgggcagggc tgctgccctc
     2581 acacctgcct gaggtggtgg ccggcctcag ctgcgccttc atcgtgctgg tcttcgtcac
     2641 tgtcttcctg gtcctgcagc tgcgctctgg ctttagtttt cggggggtga aggtgtacac
     2701 catggaccgt ggcctcatct cctacaaggg gctgccccct gaagcctggc aggaggagtg
     2761 cccgtctgac tcagaagagg acgagggccg gggcgagagg accgccttta tcaaagacca
     2821 gagcgccctc ttgccaactt tcttgtacaa agtggttgat atcaaggccg ccgacatcac
     2881 cagcctgtac aagaaagtgg ggtggagcca tccccagttc gagaagggcg gaggatctgg
     2941 cggcggaagc ggcggaggaa gctggtccca ccctcagttt gagaaaggca ccgagctggg
     3001 cagcaccatg gccagctacc cctacgacgt gcccgactac gccaaggaca acaccgtgcc
     3061 cctgaagctg atcgccctgc tggccaacgg cgagttccac tctggcgagc agctgggcga
     3121 gacactgggc atgagcagag ccgccatcaa caagcacatc cagaccctgc gggactgggg
     3181 cgtggacgtg ttcacagtgc ccggcaaggg ctacagcctg cccgagccca tccagctgct
     3241 gaacgccaag cagatcctgg gccagctgga tggcggatct gtggccgtgc tgcccgtgat
     3301 cgacagcacc aaccagtatc tgctcgaccg gatcggcgag ctgaagtccg gcgacgcctg
     3361 tatcgccgag taccagcagg ccggcagagg cggcaggggc agaaagtggt tcagcccctt
     3421 cggcgccaac ctgtacctga gcatgttctg gagactggaa cagggcccag ccgccgctat
     3481 tggcctgagc ctggtcatcg gcatcgtgat ggccgaggtg ctgcggaagc tgggcgccga
     3541 caaagtgcgc gtgaagtggc ccaacgacct gtatctgcag gaccggaagc tggccggcat
     3601 cctggtggaa ctgaccggca aaacaggcga cgccgcccag atcgtgatcg gcgctggcat
     3661 caacatggcc atgcggcggg tggaagagag cgtggtcaac cagggctgga tcaccctgca
     3721 ggaagccggc atcaacctgg accggaacac cctggccgcc atgctgatca gagagctgag
     3781 agccgccctg gaactgttcg agcaggaagg cctggccccc tacctgagca gatgggagaa
     3841 gctggacaac ttcatcaacc ggcccgtgaa actgatcatc ggcgacaaag agatcttcgg
     3901 catcagccgg ggcatcgaca agcagggggc cctgctcctg gaacaggacg gcatcatcaa
     3961 gccctggatg ggcggcgaga tcagcctgcg gagcgccgag aagtgatgac tcgagtctag
     4021 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4081 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4141 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4201 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4261 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4321 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4501 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta
     4561 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa
     4621 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     4681 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     4741 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     4801 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     4861 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     4921 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     4981 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     5041 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     5101 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     5161 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     5221 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     5281 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     5341 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     5401 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     5461 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     5521 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     5581 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
     5641 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5701 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5761 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5881 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     5941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6781 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7381 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     8101 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     8161 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     8221 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     8281 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     8341 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8401 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     8521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     8581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     8641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     8701 attaccatg
//
