LOCUS       sequence-309507-        3873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBX-1
FEATURES             Location/Qualifiers
     source          1..3873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..873
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             874..1539
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     CDS             join(1629..1697,1698..2489)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1847..1866)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(2505..2536)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2564..3442)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3469..3753
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
      481 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
      541 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
      601 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
      661 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
      721 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
      781 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
      841 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
      901 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
      961 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1021 cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga
     1081 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     1141 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     1201 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     1261 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     1321 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     1381 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc
     1441 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     1501 gagccgccga cacgggtcac gctgccgcgc cgtacgtaag aggttccaac tttcaccata
     1561 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     1621 aagctaaaat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1681 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1741 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1801 tacgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1861 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1921 atgacttggt tgagtactca ccagtcacag aaaagcatct cacggatggc atgacagtaa
     1981 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctgg
     2041 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2101 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2161 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2221 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggatcac
     2281 ttctgcgctc ggccctcccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2341 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgcatcgtag
     2401 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2461 taggtgcctc actgattaag cattggtaat gaggatcccc ctcaagtcaa aagcctccgg
     2521 tcggaggctt ttgactttct gctatggagg tcaggtatga tttttatgac aacttgacgg
     2581 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     2641 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     2701 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     2761 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     2821 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     2881 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     2941 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     3001 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     3061 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     3121 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     3181 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     3241 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     3301 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     3361 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     3421 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     3481 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     3541 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     3601 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     3661 gcgtcacact ttgctatgcc ataacatttt tatccataag attagcggat cctacctgac
     3721 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agcaccgcct
     3781 atctcgtgtg agataggcgg agatacgaac tttaagaagg agatataccc atggtcaaat
     3841 ggtcagtatt gagcgatatc tagagaattc gtc
//
