LOCUS       sequence-309508-        3674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBX-3
FEATURES             Location/Qualifiers
     source          1..3674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..873
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             874..1539
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     CDS             1629..2288
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1695..1714)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(2307..2338)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2366..3244)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3271..3555
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3478..3497
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
      481 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
      541 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
      601 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
      661 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
      721 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
      781 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
      841 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
      901 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
      961 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1021 cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga
     1081 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     1141 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     1201 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     1261 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     1321 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     1381 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacgacgc
     1441 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     1501 gagccgccga cacgggtcac gctgccgcgc cgtacgtaag aggttccaac tttcaccata
     1561 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     1621 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     1681 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     1741 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1801 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     1861 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     1921 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     1981 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     2041 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     2101 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     2161 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     2221 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     2281 gggcgtaaag atctggatcc ccctcaagtc aaaagcctcc ggtcggaggc ttttgacttt
     2341 ctgctatgga ggtcaggtat gatttttatg acaacttgac ggctacatca ttcacttttt
     2401 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
     2461 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
     2521 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
     2581 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
     2641 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
     2701 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
     2761 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
     2821 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
     2881 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
     2941 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
     3001 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
     3061 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
     3121 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
     3181 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     3241 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     3301 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     3361 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     3421 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     3481 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     3541 ctactgtttc tccatacccg tttttttggg ctagcacgcc tatctcgtgt gagataggcg
     3601 gagatacgaa ctttaacaag gagatataca catgttaaaa tggtcagtat tgagcgatat
     3661 ctagagaatt cgtc
//
