LOCUS       sequence-309509-        4206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBX-4
FEATURES             Location/Qualifiers
     source          1..4206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..873
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             874..1539
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     CDS             1628..2818
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1980..1999)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      complement(2838..2869)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2897..3775)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3802..4086
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
      481 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
      541 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
      601 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
      661 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
      721 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
      781 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
      841 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
      901 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
      961 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1021 cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga
     1081 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     1141 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     1201 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     1261 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     1321 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     1381 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc
     1441 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     1501 gagccgccga cacgggtcac gctgccgcgc cgtacgtaag aggttccaac tttcaccata
     1561 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaaga
     1621 agctaaaatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     1681 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     1741 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     1801 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     1861 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     1921 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     1981 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     2041 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     2101 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     2161 cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa
     2221 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     2281 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     2341 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     2401 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     2461 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     2521 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     2581 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     2641 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
     2701 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     2761 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     2821 ggaagggatc cccctcaagt caaaagcctc cggtcggagg tttttgactt tctgctatgg
     2881 aggtcaggta tgatttttat gacaacttga cggctacatc attcactttt tcttcacaac
     2941 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga
     3001 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
     3061 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
     3121 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
     3181 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
     3241 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
     3301 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
     3361 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
     3421 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
     3481 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
     3541 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
     3601 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
     3661 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
     3721 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
     3781 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
     3841 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
     3901 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
     3961 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccataacat
     4021 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt
     4081 ctccataccc gtttttttgg gctagcaccg cctatctcgt gtgagatagg cggagatacg
     4141 aactttaaga aggagatata cccatggtca aatggtcagt attgagcgat atctagagaa
     4201 ttcgtc
//
