LOCUS       sequence-309514-       13475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd Generation lentiviral vector expressing the codon optimized 
            SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a 
            C-terminal HA tag generated by Junko Ogawa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13475)
  AUTHORS   Ogawa J, Zhu W, Tonnu N, Singer O, Hunter T, Ryan AL, Pao GM
  TITLE     The D614G mutation in the SARS-CoV2 Spike protein increases 
            infectivity in an ACE2 receptor dependent manner.
  JOURNAL   bioRxiv. 2020 Jul 22. doi: 10.1101/2020.07.21.214932.
  PUBMED    32743569
REFERENCE   2  (bases 1 to 13475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2020 Jul 22. doi: 10.1101/2020.07.21.214932."
COMMENT     Sequence Label: pBOB-CAG-SARS-CoV2-Spike D614G-HA
FEATURES             Location/Qualifiers
     source          1..13475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2517..2896
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3174..4190
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4114..4136
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4198..4217
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             8112..8138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8282..8870
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8335..8355)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8753..8764)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9277..9457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9483..9500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9489..9713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9759..10187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9846..9865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10056..10077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10196..10216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10201..10530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10381..10516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10443..10462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10578..10625
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10644..11018
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11148..11269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11185..11204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11239..11258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11318..11334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11318..11334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11331..11353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11342..11358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11366..11372,11373..11390,11391..11396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11411..11432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11549..11566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11720..12308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11800..11819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12479..13270,13271..13339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13102..13121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13340..13444)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgtcgaca
     2521 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2581 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2641 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2701 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2761 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2821 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2881 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2941 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     3001 ggcggggggg gggggggsgc gcgccrggsg gggggggggg gggsgrgggg sggggsgggg
     3061 sgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     3121 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     3181 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     3241 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     3301 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     3361 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     3421 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     3481 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     3541 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     3601 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     3661 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     3721 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     3781 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     3841 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     3901 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     3961 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     4021 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     4081 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     4141 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     4201 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgctagt ggatccaggc
     4261 cgcctgggcc cgttaacacc atgttcgtat tccttgtcct cctccccctc gtcagctccc
     4321 agtgtgtcaa tctgacaact cgaactcagc tcccacccgc atacaccaat agttttaccc
     4381 gaggagtcta ttatcccgac aaagttttca ggtctagtgt gttgcatagc acgcaggacc
     4441 tgttcttgcc gtttttttca aatgtgacat ggttccatgc catccatgtc tctggtacga
     4501 acgggaccaa aaggtttgat aaccccgttc ttccatttaa cgacggggtt tatttcgcct
     4561 ctactgaaaa gtctaatata atacgaggtt ggatttttgg gacaacactg gactctaaaa
     4621 cgcaatctct cctcatagta aataatgcga cgaatgtcgt aatcaaagta tgtgagtttc
     4681 agttttgcaa cgaccctttt cttggtgtat actatcacaa gaacaacaag tcttggatgg
     4741 aaagtgaatt tagagtctac agctccgcaa acaactgtac ctttgagtac gtcagtcagc
     4801 ccttccttat ggacctcgaa ggcaaacagg ggaacttcaa gaatctgcgc gagttcgtgt
     4861 tcaagaatat cgacggctat ttcaagatat attcaaaaca tacaccaatc aatttggtac
     4921 gcgatttgcc gcagggcttc tctgctcttg agcctctcgt agatttgcct atagggatca
     4981 atattactcg ctttcaaact ctccttgccc ttcatcggtc ctacctgaca ccaggcgaca
     5041 gctccagtgg gtggacagct ggtgcagcgg cttactacgt cggctacctc caacccagga
     5101 cttttttgct gaaatataat gagaatggta cgattactga cgccgtcgat tgtgccctcg
     5161 acccattgag cgaaactaaa tgcaccctga aaagctttac ggttgagaaa gggatatatc
     5221 agacatctaa ttttcgcgtt caacccacgg aatccattgt acgatttccc aacattacca
     5281 atttgtgtcc ttttggtgag gtatttaatg caaccagatt cgcgagtgtg tatgcctgga
     5341 ataggaaaag aatttctaac tgtgtagcag actacagcgt tctgtataat agtgcgtcct
     5401 ttagtacgtt caagtgctac ggagtaagcc cgacaaagtt gaacgatttg tgtttcacta
     5461 acgtctatgc cgatagcttc gtcatcaggg gagacgaagt aaggcaaatt gcaccaggcc
     5521 agaccggaaa aatagccgac tacaactata agttgccgga cgatttcacc gggtgtgtca
     5581 tcgcgtggaa tagtaataac ctggactcca aggtgggcgg caactacaac tacctgtacc
     5641 gattgttccg caagtctaac ctcaagccct ttgaacgaga catatccacc gagatatacc
     5701 aagcgggaag cactccctgt aatggcgttg aaggtttcaa ctgctatttt cctctccagt
     5761 cctatggctt ccaacctacc aatggagtag gctatcaacc gtatcgagtc gtggtgctga
     5821 gtttcgagct cctgcacgct ccagcgaccg tttgtggtcc taagaaaagt acaaacctcg
     5881 taaaaaataa gtgcgtaaac tttaacttta acggtctcac aggaacggga gttcttacag
     5941 agtccaataa gaagttcctg ccctttcagc agtttgggcg cgacatcgcg gacaccacag
     6001 atgcagtcag ggacccgcag actcttgaga tcctggacat caccccatgc tcttttggcg
     6061 gtgtgagtgt cattacaccc gggaccaaca ccagcaatca ggttgctgtg ctctatcaag
     6121 gcgtcaactg tacggaagtc ccagttgcaa tacacgcgga ccagttgacg ccgacgtggc
     6181 gggtttattc taccggctca aacgtctttc aaactagagc cggttgtttg atcggggcgg
     6241 agcatgtgaa caattcatac gaatgtgata ttcccatagg agccggaata tgtgcttctt
     6301 accagaccca aaccaactct ccgagacgag cccggtccgt agccagtcaa agcataattg
     6361 cgtacaccat gagcctcggt gcagaaaact cagttgcgta ctcaaataac tccattgcca
     6421 tcccaacaaa ttttaccata tcagtgacca ctgaaatcct tccagtgagc atgactaaaa
     6481 caagtgtaga ttgcacaatg tatatttgcg gagactcaac tgagtgctct aacctcctgt
     6541 tgcagtacgg ttcattctgt acccagctca atcgcgcact tacgggaata gcagtagaac
     6601 aagataagaa tactcaggaa gtcttcgctc aagtaaagca aatctacaag acgcccccca
     6661 ttaaggactt tgggggtttt aattttagcc aaatactccc ggaccccagt aaaccctcta
     6721 agaggtcatt tatagaagac ctgttgttta ataaagttac tctggccgat gctggcttta
     6781 ttaaacaata cggtgattgt ttgggcgaca tcgcggcgcg ggatctgata tgcgcccaaa
     6841 aatttaacgg tctcactgtg ttgccgcctt tgctcacaga cgagatgata gctcagtaca
     6901 catcagcgtt gttggcgggt accataacgt ctggatggac attcggagca ggggcggcct
     6961 tgcaaatacc attcgcaatg caaatggcat acaggttcaa cgggataggt gttacacaga
     7021 atgtacttta cgagaaccag aaattgattg caaaccagtt taactctgcg attggcaaaa
     7081 ttcaggatag cctcagctcc accgcgtctg cactgggaaa actccaagat gttgtgaacc
     7141 aaaatgccca agccctcaat acacttgtga agcaactctc cagcaatttc ggtgcaataa
     7201 gtagtgtgct gaacgacata ctttctcggt tggacaaagt agaggcggag gtgcaaatcg
     7261 acagactcat aacaggtcga ctgcaatcat tgcaaacgta tgttactcag caattgatac
     7321 gggcggcgga gattcgagcg tctgccaacc tcgccgcgac taaaatgtct gagtgtgtgc
     7381 tcggtcagtc aaaacgcgtg gacttctgtg gaaagggata ccatctcatg tccttccctc
     7441 agtctgcccc tcacggagta gtctttcttc atgtgaccta tgttccagca caggagaaaa
     7501 attttaccac agcgcccgcg atatgccacg atggcaaagc tcattttccg cgagagggag
     7561 tatttgtctc taacggcacc cattggtttg taacccagcg caatttttac gaacctcaga
     7621 taatcaccac tgacaacacc tttgttagtg gtaattgtga tgtagtgatc gggatagtga
     7681 acaatacagt gtacgacccc ctgcaacctg agctggactc ctttaaagaa gaactcgaca
     7741 agtacttcaa aaaccacact tccccggatg tcgatttggg tgatatatct ggcatcaatg
     7801 cgagtgtagt caacatacag aaggaaatcg acaggctcaa tgaagtagca aaaaacctga
     7861 atgaatccct catagacctc caagagctgg gcaaatatga gcagtacatt aaatggccct
     7921 ggtacatttg gctgggcttc atcgctggcc tgatagcgat agtgatggtt actattatgc
     7981 tctgctgtat gacgagttgt tgcagctgcc tcaaagggtg ttgttcttgc ggctcctgtt
     8041 gtaagttcga cgaggatgat tcagagcccg ttcttaaggg tgtaaaactc cattacactg
     8101 gaggaggagg ttacccatac gatgttccag attacgctta gtaaactagt aacgtctcga
     8161 gtttaaacct ggaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     8221 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcaga
     8281 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8341 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8401 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8461 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8521 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8581 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8641 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     8701 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8761 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8821 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
     8881 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8941 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     9001 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     9061 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     9121 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     9181 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     9241 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     9301 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9361 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9421 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg ccgctttaaa cccgctgatc
     9481 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9541 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9601 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9661 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     9721 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     9781 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9841 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9901 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9961 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10021 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10081 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    10141 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
    10201 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10261 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10321 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    10381 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10441 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
    10501 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
    10561 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
    10621 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
    10681 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
    10741 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
    10801 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
    10861 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
    10921 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
    10981 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    11041 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    11101 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    11161 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    11221 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    11281 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    11341 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    11401 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    11461 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11521 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    11581 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    11641 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    11701 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    11761 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11821 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11881 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11941 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    12061 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12121 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    12181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12241 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    12301 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    12361 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    12421 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    12481 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    12541 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    12601 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    12661 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    12721 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    12781 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12841 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12901 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12961 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    13021 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    13081 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    13141 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    13201 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    13261 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    13321 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    13381 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    13441 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
