LOCUS       sequence-309519-        8130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-SARS-CoV-2 NSP13
FEATURES             Location/Qualifiers
     source          1..8130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    422..446
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2253..2277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2324..2347
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2384..2407
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2438..2464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2438..2464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2465..2812,2813..2815,2816..3424)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(3468..3485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3474..3698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3831..3850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    3982..4029
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4037..5059
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4037..5059
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5189..5310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5226..5245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5280..5299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5359..5375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5359..5375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5372..5394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5383..5399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5407..5413,5414..5431,5432..5437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5452..5473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5590..5607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5761..6349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5841..5860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6520..7311,7312..7380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7143..7162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7381..7485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7560..7579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7751..8130
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
      421 aacaagtttg tacaaaaaag ttggcatggc cgtgggcgct tgcgtgctgt gcaactctca
      481 gaccagcctg agatgtggag cttgcatccg caggcctttc ctgtgctgca agtgctgcta
      541 cgaccacgtg atcagcacct cccacaagct ggtgctgtcc gtgaacccat acgtgtgcaa
      601 cgctcctggt tgtgacgtga ctgacgtgac ccagctgtac ctgggcggaa tgagctacta
      661 ctgcaagtcc cacaagcccc ctatcagctt cccactgtgc gctaacggcc aggtgttcgg
      721 actgtacaag aacacctgcg tgggctccga caacgtgacc gacttcaacg ccatcgctac
      781 ctgcgactgg accaacgccg gagactacat cctggctaac acctgcaccg aaaggctgaa
      841 gctgttcgcc gctgagaccc tgaaggccac cgaggaaacc ttcaagctgt cttacggtat
      901 cgctaccgtg cgtgaggtgc tgtctgaccg cgaactgcac ctgtcttggg aagtgggcaa
      961 gccaagacca cccctgaacc gtaactacgt gttcaccgga tacagggtga ccaagaacag
     1021 caaggtgcag atcggagagt acaccttcga aaagggagac tacggtgacg ccgtggtgta
     1081 cagaggaacc accacctaca agctgaacgt gggtgactac ttcgtgctga cctcccacac
     1141 cgtgatgcca ctgtctgctc ccaccctggt gcctcaggaa cactacgtgc gcatcaccgg
     1201 actgtaccca accctgaaca tctccgacga gttcagctcc aacgtggcca actaccagaa
     1261 agtgggcatg cagaagtact ccaccctgca gggacctcca ggtaccggca agtctcactt
     1321 cgccatcggt ctggctctgt actacccctc tgcccgtatc gtgtacactg cttgttctca
     1381 cgctgctgtg gatgctctgt gcgagaaggc tctgaagtac ctgcctatcg acaagtgctc
     1441 ccgcatcatc ccagccagag ctcgtgtgga atgcttcgac aagttcaagg tgaactctac
     1501 cctggagcag tacgtgttct gcaccgtgaa cgccctgcct gaaaccaccg ctgacatcgt
     1561 ggtgttcgac gagatcagca tggccaccaa ctacgacctg tctgtggtga acgctagact
     1621 gagggctaag cactacgtgt acatcggtga cccagctcag ctgcctgctc ctcgtaccct
     1681 gctgaccaag ggcaccctgg agccagaata cttcaacagc gtgtgccgcc tgatgaagac
     1741 catcggtcca gacatgttcc tgggtacctg ccgccgctgc cctgctgaaa tcgtggacac
     1801 cgtgtctgct ctggtgtacg acaacaagct gaaggcccac aaggacaaga gcgcccagtg
     1861 cttcaagatg ttctacaagg gagtgatcac ccacgacgtg tctagcgcca tcaacaggcc
     1921 ccagatcggt gtggtgcgcg agttcctgac caggaacccc gcctggagaa aggccgtgtt
     1981 catctcccct tacaactctc agaacgccgt ggcttccaag atcctgggtc tgcctaccca
     2041 gaccgtggac tcctctcagg gctctgaata cgactacgtg atcttcaccc agaccaccga
     2101 gaccgcccac agctgcaacg tgaacaggtt caacgtggcc atcaccagag ctaaagtggg
     2161 aatcctgtgc atcatgagcg acagagacct gtacgacaag ctgcagttca cctccctgga
     2221 gatcccccgc aggaacgtgg ctaccctgca gtacccaact ttcttgtaca aagtggttga
     2281 tatcaaggcc gccgacatca ccagcctgta caagaaagtg gggtggagcc atccccagtt
     2341 cgagaagggc ggaggatctg gcggcggaag cggcggagga agctggtccc accctcagtt
     2401 tgagaaaggc accgagctgg gcagcaccat ggccagctac ccctacgacg tgcccgacta
     2461 cgccaaggac aacaccgtgc ccctgaagct gatcgccctg ctggccaacg gcgagttcca
     2521 ctctggcgag cagctgggcg agacactggg catgagcaga gccgccatca acaagcacat
     2581 ccagaccctg cgggactggg gcgtggacgt gttcacagtg cccggcaagg gctacagcct
     2641 gcccgagccc atccagctgc tgaacgccaa gcagatcctg ggccagctgg atggcggatc
     2701 tgtggccgtg ctgcccgtga tcgacagcac caaccagtat ctgctcgacc ggatcggcga
     2761 gctgaagtcc ggcgacgcct gtatcgccga gtaccagcag gccggcagag gcggcagggg
     2821 cagaaagtgg ttcagcccct tcggcgccaa cctgtacctg agcatgttct ggagactgga
     2881 acagggccca gccgccgcta ttggcctgag cctggtcatc ggcatcgtga tggccgaggt
     2941 gctgcggaag ctgggcgccg acaaagtgcg cgtgaagtgg cccaacgacc tgtatctgca
     3001 ggaccggaag ctggccggca tcctggtgga actgaccggc aaaacaggcg acgccgccca
     3061 gatcgtgatc ggcgctggca tcaacatggc catgcggcgg gtggaagaga gcgtggtcaa
     3121 ccagggctgg atcaccctgc aggaagccgg catcaacctg gaccggaaca ccctggccgc
     3181 catgctgatc agagagctga gagccgccct ggaactgttc gagcaggaag gcctggcccc
     3241 ctacctgagc agatgggaga agctggacaa cttcatcaac cggcccgtga aactgatcat
     3301 cggcgacaaa gagatcttcg gcatcagccg gggcatcgac aagcaggggg ccctgctcct
     3361 ggaacaggac ggcatcatca agccctggat gggcggcgag atcagcctgc ggagcgccga
     3421 gaagtgatga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3481 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3541 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3601 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3661 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     3721 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3841 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3901 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3961 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     4021 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     4081 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     4141 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     4201 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     4261 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     4321 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     4381 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     4441 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     4501 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     4561 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     4621 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     4681 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     4741 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     4801 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     4861 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     4921 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     4981 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     5041 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     5101 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5161 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5221 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5281 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5341 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5401 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5461 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5521 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5581 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7501 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     7561 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     7621 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     7681 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     7741 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     7801 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     7861 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     7921 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     7981 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8041 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8101 atctacgtat tagtcatcgc tattaccatg
//
