LOCUS       sequence-30954-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K
  TITLE     Consideration about negative controls for LC3 and expression vectors
            for four colored fluorescent protein-LC3 negative controls.
  JOURNAL   Autophagy. 2008 Jan 1. 4(1):131-4.
  PUBMED    18000393
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2008 Jan 1. 4(1):131-4."
COMMENT     Sequence Label: pEX-CFP-hLC3WT
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..441)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(499..520)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     848..867
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattgagc tcccgggctc
       61 gacttacact gacaatttca tcccgaacgt ctcctgggag gcgtagacca tgtacaggaa
      121 tccatcttca tctttctcac tctcatacac ctctgagatt ggtgtggaga cgctgaccat
      181 gctgtgtccg ttcaccaaca ggaagaaggc ctgattagca ttgagctgta agcgccttct
      241 aattatcttg atgagctcac tcatgttgac atggtcaggt acaaggaact ttgttttatc
      301 cagaacagga agctgcttct cacccttgta tcgttctatt atcaccggga ttttggttgg
      361 atgctgctct cgaataagtc ggacatcttc tactctttgt tcgaaggtgc ggcgctgctt
      421 gaaggtcttc tccgacggca tagatctgag tccggacttg tacagctcgt ccatgccgag
      481 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      541 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      601 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      661 gttgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata
      721 gacgttgtgg ctgatgtagt tgtactccag cttgtgcccc angatgttgc cgtcctcctt
      781 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      841 ggnnngngtc ttgtagttgc cgtcgtcctt gaagaanang gtgcgctcct ggacgtagcc
      901 ttcgggcatg gcggacttga agantcgtgc tg
//
