LOCUS       sequence-309548-        9105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-NRP1
FEATURES             Location/Qualifiers
     source          1..9105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3608..3632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3679..3702
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3739..3762
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3793..3819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3793..3819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3820..4167,4168..4170,4171..4779)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4823..4840)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4829..5053
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5186..5205)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5337..5384
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5392..6414
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5392..6414
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6544..6665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6581..6600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6635..6654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6714..6730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6714..6730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6727..6749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6738..6754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6762..6768,6769..6786,6787..6792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6945..6962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7116..7704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7196..7215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7875..8666,8667..8735))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8498..8517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8736..8840)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8915..8934)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac catggagagg
      841 gggctgccgc tcctctgcgc cgtgctcgcc ctcgtcctcg ccccggccgg cgcttttcgc
      901 aacgataaat gtggcgatac tataaaaatt gaaagccccg ggtaccttac atctcctggt
      961 tatcctcatt cttatcaccc aagtgaaaaa tgcgaatggc tgattcaggc tccggaccca
     1021 taccagagaa ttatgatcaa cttcaaccct cacttcgatt tggaggacag agactgcaag
     1081 tatgactacg tggaagtctt cgatggagaa aatgaaaatg gacattttag gggaaagttc
     1141 tgtggaaaga tagcccctcc tcctgttgtg tcttcagggc catttctttt tatcaaattt
     1201 gtctctgact acgaaacaca tggtgcagga ttttccatac gttatgaaat tttcaagaga
     1261 ggtcctgaat gttcccagaa ctacacaaca cctagtggag tgataaagtc ccccggattc
     1321 cctgaaaaat atcccaacag ccttgaatgc acttatattg tctttgcacc aaagatgtca
     1381 gagattatcc tggaatttga aagctttgac ctggagcctg actcaaatcc tccagggggg
     1441 atgttctgtc gctacgaccg gctagaaatc tgggatggat tccctggtgt tggccctcac
     1501 attgggcgtt actgtggaca gaaaacacca ggtcgaatcc gatcctcatc gggcattctc
     1561 tccatggttt tttacaccga cagcgcgata gcaaaagaag gtttctcagc aaactacagt
     1621 gttttgcaga gcagtgtctc agaagatttc aaatgtatgg aagctctggg catggaatca
     1681 ggagaaattc attctgacca gatcacagct tcttcccagt atagcaccaa ctggtctgca
     1741 gagcgctccc gcctgaacta ccctgagaat gggtggactc ccggagagga ttcctaccga
     1801 gagtggatac aggtagactt gggccttctg cgctttgtca cggctgtcgg gacacagggc
     1861 gccatttcaa aagaaaccaa gaagaaatat tatgtcaaga cttacaagat cgacgttagc
     1921 tccaacgggg aagactggat caccataaaa gaaggaaaca aacctgttct ctttcaggga
     1981 aacaccaacc ccacagatgt tgtggttgca gtattcccca aaccactgat aactcgattt
     2041 gtccgaatca agcctgcaac ttgggaaact ggcatatcta tgagatttga agtatacggt
     2101 tgcaagataa cagattatcc ttgctctgga atgttgggta tggtgtctgg acttatttct
     2161 gactcccaga tcacatcatc caaccaaggg gacagaaact ggatgcctga aaacatccgc
     2221 ctggtaacca gtcgctctgg ctgggcactt ccacccgcac ctcattccta catcaatgag
     2281 tggctccaaa tagacctggg ggaggagaag atcgtgaggg gcatcatcat tcagggtggg
     2341 aagcaccgag agaacaaggt gttcatgagg aagttcaaga tcgggtacag caacaacggc
     2401 tcggactgga agatgatcat ggatgacagc aaacgcaagg cgaagtcttt tgagggcaac
     2461 aacaactatg atacacctga gctgcggact tttccagctc tctccacgcg attcatcagg
     2521 atctaccccg agagagccac tcatggcgga ctggggctca gaatggagct gctgggctgt
     2581 gaagtggaag cccctacagc tggaccgacc actcccaacg ggaacttggt ggatgaatgt
     2641 gatgacgacc aggccaactg ccacagtgga acaggtgatg acttccagct cacaggcggc
     2701 accactgtgc cggccacaga aaagcccacg gtcatagaca gcaccataca atcagagttt
     2761 ccaacatatg gttttaactg tgaatttggc tggggctctc acaagacctt ctgccactgg
     2821 gaacatgaca atcacgtgca gctcaagtgg agtgtgttga ccagcaagac gggacccatt
     2881 caggatcaca caggagatgg caacttcatc tattcccaag ctgacgaaaa tcagaagggc
     2941 aaagtggctc gcctggtgag ccctgtggtt tattcccaga actctgccca ctgcatgacc
     3001 ttctggtatc acatgtctgg gtcccacgtc ggcacactca gggtcaaact gcgctaccag
     3061 aagccagagg agtacgatca gctggtctgg atggccattg gacaccaagg tgaccactgg
     3121 aaggaagggc gtgtcttgct ccacaagtct ctgaaacttt atcaggtgat tttcgagggc
     3181 gaaatcggaa aaggaaacct tggtgggatt gctgtggatg acattagtat taataaccac
     3241 atttcacaag aagattgtgc aaaaccagca gacctggata aaaagaaccc agaaattaaa
     3301 attgatgaaa cagggagcac gccaggatac gaaggtgaag gagaaggtga caagaacatc
     3361 tccaggaagc caggcaatgt gttgaagacc ttagacccca tcctcatcac catcatagcc
     3421 atgagtgccc tgggggtcct cctgggggct gtctgtgggg tcgtgctgta ctgtgcctgt
     3481 tggcataatg ggatgtcaga aagaaacttg cctgccctgg agaactataa ctttgaactt
     3541 gtggatggtg tgaagttgaa aaaagacaaa ctgaatacac agagtactta ttcggaggca
     3601 aagcttgacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     3661 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     3721 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     3781 accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac cgtgcccctg
     3841 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggcgagaca
     3901 ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga ctggggcgtg
     3961 gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca gctgctgaac
     4021 gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac
     4081 agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga cgcctgtatc
     4141 gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag ccccttcggc
     4201 gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc cgctattggc
     4261 ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa
     4321 gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc cggcatcctg
     4381 gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac
     4441 atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac cctgcaggaa
     4501 gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc
     4561 gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg ggagaagctg
     4621 gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat cttcggcatc
     4681 agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat catcaagccc
     4741 tggatgggcg gcgagatcag cctgcggagc gccgagaagt gatgactcga gtctagaggg
     4801 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4861 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4921 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4981 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5041 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     5101 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5161 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5221 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5281 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     5341 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     5401 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5461 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5521 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5581 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5641 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5701 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5761 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5821 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     5881 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5941 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     6001 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     6061 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     6121 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     6181 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     6241 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     6301 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     6361 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     6421 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6481 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6541 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6601 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6661 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8161 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8461 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     8881 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8941 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     9001 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     9061 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
