LOCUS       sequence-30955-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K
  TITLE     Consideration about negative controls for LC3 and expression vectors
            for four colored fluorescent protein-LC3 negative controls.
  JOURNAL   Autophagy. 2008 Jan 1. 4(1):131-4.
  PUBMED    18000393
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2008 Jan 1. 4(1):131-4."
COMMENT     Sequence Label: pEX-CFP-hLC3-Delta-G
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..440)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFMKLSV"
     primer_bind     complement(498..519)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caccagccac caccttctga taggcagcct gcacctgagg agtgaattga gctccngggc
       61 tcgacttaca ctgacaattt catgaacgtc tcctgggagg cgtagaccat gtacaggaat
      121 ccatcttcat ctttctcact ctcatacacc tctgagattg gtgtggagac gctgaccatg
      181 ctgtgtccgt tcaccaacag gaagaaggcc tgattagcat tgagctgtaa gcgccttcta
      241 attatcttga tgagctcact catgttgaca tggtcaggta caaggaactt tgttttatcc
      301 agaacaggaa gctgcttctc acccttgtat cgttctatta tcaccgggat tttggttgga
      361 tgctgctctc gaataagtcg gacatcttct actctttgtt cgaaggtgcg gcgctgcttg
      421 aaggtcttct ccgacggcat agatctgagt ccggacttgt acagctcgtc catgccgaga
      481 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      541 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      601 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      661 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      721 acgttgtggc tgatgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      781 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
//
