LOCUS       sequence-309552-        8745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x."
COMMENT     Sequence Label: MAC-ACE2
FEATURES             Location/Qualifiers
     source          1..8745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3248..3272)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3319..3342
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3379..3402
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3433..3459
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3433..3459
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3460..3807,3808..3810,3811..4419)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4463..4480)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4469..4693
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4826..4845)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4977..5024
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5032..6054
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5032..6054
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6184..6305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6221..6240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6275..6294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6354..6370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6354..6370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6367..6389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6378..6394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6402..6408,6409..6426,6427..6432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6447..6468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6585..6602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6756..7344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6836..6855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7515..8306,8307..8375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8138..8157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8376..8480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8555..8574)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac catgtcaagc
      841 tcttcctggc tccttctcag ccttgttgct gtaactgctg ctcagtccac cattgaggaa
      901 caggccaaga catttttgga caagtttaac cacgaagccg aagacctgtt ctatcaaagt
      961 tcacttgctt cttggaatta taacaccaat attactgaag agaatgtcca aaacatgaat
     1021 aatgctgggg acaaatggtc tgccttttta aaggaacagt ccacacttgc ccaaatgtat
     1081 ccactacaag aaattcagaa tctcacagtc aagcttcagc tgcaggctct tcagcaaaat
     1141 gggtcttcag tgctctcaga agacaagagc aaacggttga acacaattct aaatacaatg
     1201 agcaccatct acagtactgg aaaagtttgt aacccagata atccacaaga atgcttatta
     1261 cttgaaccag gtttgaatga aataatggca aacagtttag actacaatga gaggctctgg
     1321 gcttgggaaa gctggagatc tgaggtcggc aagcagctga ggccattata tgaagagtat
     1381 gtggtcttga aaaatgagat ggcaagagca aatcattatg aggactatgg ggattattgg
     1441 agaggagact atgaagtaaa tggggtagat ggctatgact acagccgcgg ccagttgatt
     1501 gaagatgtgg aacatacctt tgaagagatt aaaccattat atgaacatct tcatgcctat
     1561 gtgagggcaa agttgatgaa tgcctatcct tcctatatca gtccaattgg atgcctccct
     1621 gctcatttgc ttggtgatat gtggggtaga ttttggacaa atctgtactc tttgacagtt
     1681 ccctttggac agaaaccaaa catagatgtt actgatgcaa tggtggacca ggcctgggat
     1741 gcacagagaa tattcaagga ggccgagaag ttctttgtat ctgttggtct tcctaatatg
     1801 actcaaggat tctgggaaaa ttccatgcta acggacccag gaaatgttca gaaagcagtc
     1861 tgccatccca cagcttggga cctggggaag ggcgacttca ggatccttat gtgcacaaag
     1921 gtgacaatgg acgacttcct gacagctcat catgagatgg ggcatatcca gtatgatatg
     1981 gcatatgctg cacaaccttt tctgctaaga aatggagcta atgaaggatt ccatgaagct
     2041 gttggggaaa tcatgtcact ttctgcagcc acacctaagc atttaaaatc cattggtctt
     2101 ctgtcacccg attttcaaga agacaatgaa acagaaataa acttcctgct caaacaagca
     2161 ctcacgattg ttgggactct gccatttact tacatgttag agaagtggag gtggatggtc
     2221 tttaaagggg aaattcccaa agaccagtgg atgaaaaagt ggtgggagat gaagcgagag
     2281 atagttgggg tggtggaacc tgtgccccat gatgaaacat actgtgaccc cgcatctctg
     2341 ttccatgttt ctaatgatta ctcattcatt cgatattaca caaggaccct ttaccaattc
     2401 cagtttcaag aagcactttg tcaagcagct aaacatgaag gccctctgca caaatgtgac
     2461 atctcaaact ctacagaagc tggacagaaa ctgttcaata tgctgaggct tggaaaatca
     2521 gaaccctgga ccctagcatt ggaaaatgtt gtaggagcaa agaacatgaa tgtaaggcca
     2581 ctgctcaact actttgagcc cttatttacc tggctgaaag accagaacaa gaattctttt
     2641 gtgggatgga gtaccgactg gagtccatat gcagaccaaa gcatcaaagt gaggataagc
     2701 ctaaaatcag ctcttggaga taaagcatat gaatggaacg acaatgaaat gtacctgttc
     2761 cgatcatctg ttgcatatgc tatgaggcag tactttttaa aagtaaaaaa tcagatgatt
     2821 ctttttgggg aggaggatgt gcgagtggct aatttgaaac caagaatctc ctttaatttc
     2881 tttgtcactg cacctaaaaa tgtgtctgat atcattccta gaactgaagt tgaaaaggcc
     2941 atcaggatgt cccggagccg tatcaatgat gctttccgtc tgaatgacaa cagcctagag
     3001 tttctgggga tacagccaac acttggacct cctaaccagc cccctgtttc catatggctg
     3061 attgtttttg gagttgtgat gggagtgata gtggttggca ttgtcatcct gatcttcact
     3121 gggatcagag atcggaagaa gaaaaataaa gcaagaagtg gagaaaatcc ttatgcctcc
     3181 atcgatatta gcaaaggaga aaataatcca ggattccaaa acactgatga tgttcagacc
     3241 tcctttgacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     3301 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     3361 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     3421 accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac cgtgcccctg
     3481 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggcgagaca
     3541 ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga ctggggcgtg
     3601 gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca gctgctgaac
     3661 gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac
     3721 agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga cgcctgtatc
     3781 gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag ccccttcggc
     3841 gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc cgctattggc
     3901 ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa
     3961 gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc cggcatcctg
     4021 gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac
     4081 atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac cctgcaggaa
     4141 gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc
     4201 gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg ggagaagctg
     4261 gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat cttcggcatc
     4321 agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat catcaagccc
     4381 tggatgggcg gcgagatcag cctgcggagc gccgagaagt gatgactcga gtctagaggg
     4441 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4501 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4561 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4621 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4681 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4741 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4801 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4861 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4921 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     4981 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     5041 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5101 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5161 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5221 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5281 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5341 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5401 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5461 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     5521 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5581 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5641 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5701 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5761 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5821 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5881 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5941 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     6001 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     6061 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6121 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6181 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6301 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7501 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7561 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7621 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7681 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7741 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7801 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7861 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7921 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7981 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8041 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8101 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8161 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8221 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8281 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8341 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8401 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8461 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     8521 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8581 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     8641 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     8701 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
