LOCUS       sequence-309565-        3685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High Copy number plasmid containing smCBA insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G,
            M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M 
            Simpson E, R Leavitt B
  TITLE     New MiniPromoter Ple389 (ADORA2A) drives selective expression in 
            medium spiny neurons in mice and non-human primates.
  JOURNAL   Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y.
  PUBMED    39548191
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-15"; volume: "14(1):28194. doi"; pages: " 
            10.1038/s41598-024-79004-y"
COMMENT     Sequence Label: pEMS2075
FEATURES             Location/Qualifiers
     source          1..3685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(467..486)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        complement(743..1020)
                     /label=chicken beta-actin promoter
     enhancer        1022..1325
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1464..1480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1464..1480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1477..1499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1488..1504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1512..1518,1519..1536,1537..1542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1557..1578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1695..1712)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1866..2454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1946..1965)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2625..3416,3417..3485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3248..3267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3486..3590)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3658..3676
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tattgggggc gcgcctttgc caaaatgatg agacagcaca ataaccagca
      481 cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag
      541 ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac
      601 caagcgctaa ttacagcccg gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa
      661 cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg
      721 aaggcagcgt cgcagcgact cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc
      781 gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc gctctgattg gctgccgccg
      841 cacctctccg cctcgccccs ccccsccccy csccccsccc csccccsccy ggcgcgcgcc
      901 cccccccccc ccccgccccc atcgctgcac aaaataatta aaaaataaat aaatacaaaa
      961 ttgggggtgg ggaggggggg gagatgggga gagtgaagca gaacgtgggg ctcacctcga
     1021 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     1081 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     1141 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatagtcca
     1201 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     1261 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     1321 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     1381 aactagggta ccggccggcc attgggatcg gatcccgggc ccgtcgactg cagaggcctg
     1441 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1501 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1561 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1621 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1681 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1741 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1801 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1861 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1921 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1981 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2041 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2101 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2161 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2221 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2281 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2341 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2401 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2461 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2521 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2581 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2641 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2701 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2761 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2821 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2881 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2941 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3001 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3061 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3121 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3181 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3241 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3301 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3361 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3421 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3481 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3541 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3601 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3661 aggcgtatca cgaggccctt tcgtc
//
