LOCUS       sequence-309572-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector of constitutive expression of mNeonGreen3A(1-10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Zhou S, Feng S, Brown D, Huang B
  TITLE     Improved yellow-green split fluorescent proteins for protein 
            labeling and signal amplification.
  JOURNAL   PLoS One. 2020 Nov 23;15(11):e0242592. doi: 
            10.1371/journal.pone.0242592. eCollection 2020.
  PUBMED    33227014
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-11-23"; volume: "15(11):e0242592. doi"; pages: " 
            10.1371/journal.pone.0242592. eCollection 2020"
COMMENT     Sequence Label: pSFFV_mNG3A(1-10)
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      738..747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1187..1775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1240..1260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1658..1669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1778..1794)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1779..1795)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1885..2118
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2143..2161)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2144..2161)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2451..2470)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2684..2703)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2803..2825
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2863..2881)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2949..3053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3054..3122,3123..3914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3272..3291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4085..4673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4574..4593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4827..4844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4914..4934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4919..5248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5099..5234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6382..6447
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6577..6597
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7022..7156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7059..7078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7113..7132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7324..7957
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8004..8129
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8626..8859
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9044..9088
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9373..9490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tctgcaggat atcaagcttg cggtaccgcg ggcccgggat
      481 ccatggtgag caagggtgag gaggataaca tggcctctct cccagcgact catgagttac
      541 acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc
      601 caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggcgacctc cagttctccc
      661 cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg
      721 ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc catcgcacaa
      781 tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc
      841 acatcaaagg agaggcccag gtgatgggga ctggcttccc tgctgacggt cctgtgatga
      901 ccaacacgct gaccgctgcg gacttgtgcg tgtcgaagat gacttacccc aacgacaaaa
      961 ccatcatcag tacctttaag tggagttaca ccactgtaaa tggcaaacgc taccggagca
     1021 ctgcgcggac cacctacacc tttgccaagc caatggcggc taaatatctg aagaaccagc
     1081 cgatgtacgt gctccgtaag acggagctca agcactccat gtaataagcg gccgcgactc
     1141 tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga
     1201 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     1261 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     1321 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     1381 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     1441 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     1501 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     1561 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     1621 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     1681 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     1741 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa
     1801 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     1861 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     1921 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     1981 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2041 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2101 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     2161 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     2221 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     2281 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     2341 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     2401 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     2461 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     2521 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     2581 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     2641 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     2701 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     2761 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     2821 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     2881 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2941 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3001 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3061 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4741 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4801 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     4921 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4981 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5041 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5221 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     5281 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     5341 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     5401 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     5461 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     5521 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     5581 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     5641 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     5701 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     5761 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     5821 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     5881 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     5941 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     6001 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     6061 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     6121 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     6181 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     6241 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     6301 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     6361 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     6421 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     6481 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     6541 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     6601 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     6661 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     6721 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     6781 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     6841 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     6901 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     6961 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7021 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7141 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     7201 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     7261 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     7321 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     7381 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7441 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7501 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7561 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7621 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7681 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7741 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7921 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7981 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8041 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8101 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8161 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8221 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8281 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8341 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8401 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8461 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8521 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8581 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8641 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8701 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8761 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8821 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8881 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8941 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9001 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9061 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9121 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9181 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9241 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9301 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
     9361 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     9421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     9481 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
     9541 tatcgaattc ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
     9601 ggaatgaaag accccacctg taggtttggc aagctagctg ca
//
