LOCUS       sequence-309573-       16665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control plasmid for knockout of AP3 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT,
            Stuttmann J, Marillonnet S
  TITLE     High-efficiency genome editing in plants mediated by a Cas9 gene 
            containing multiple introns.
  JOURNAL   Plant Commun. 2020 Nov 23;2(2):100135. doi: 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8.
  PUBMED    33898975
REFERENCE   2  (bases 1 to 16665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2020-11-23"; volume: "2(2):100135. doi"; pages: " 
            10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8"
COMMENT     Sequence Label: pAGM55361
FEATURES             Location/Qualifiers
     source          1..16665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             340..969
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1398..2471
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2537..2731
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2793..2897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2898..3692
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3863..4451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4352..4371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4587..4611
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4776..4955
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4991..5046
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      5607..6314
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             8017..8037
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13770..13790
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      13817..14069
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        14213..14288
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             15500..16195
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgccagccgt gcggctgcat gaaatcctgg ccggtttgtc
      181 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
      241 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
      301 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
      361 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
      421 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
      481 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
      541 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
      601 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
      661 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
      721 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
      781 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
      841 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
      901 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
      961 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     1021 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     1081 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     1141 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     1201 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     1261 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     1321 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     1381 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     1441 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     1501 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     1561 aatcccggca accgccggca gccggtgcgt cgtcgattag gaagccgccc aagggcgacg
     1621 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     1681 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     1741 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     1801 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     1861 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     1921 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     1981 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     2041 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     2101 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     2161 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     2221 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     2281 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     2341 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     2401 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     2461 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     2521 gaaaaagctt ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     2581 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     2641 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     2701 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaacagc
     2761 tgcaaaaagc gcccacatca aggctccgag tgcgcggaac ccctatttgt ttatttttct
     2821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2881 attgaaaaag gaagagtatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     2941 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     3001 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     3061 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     3121 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     3181 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
     3241 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     3301 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     3361 attgcgaaaa ctgggaagag gacactccat ttaaagatcc gcgcgagctg tatgattttt
     3421 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     3481 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     3541 cggacaagtg gtatgacatt gccttctgcg tccggtcgct cagggaggat atcggggaag
     3601 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     3661 aatattatat tttactggat gaattgtttt agctgtcaga ccaagtttac tcatatatac
     3721 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3781 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3841 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3901 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3961 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4021 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4081 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4141 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4201 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4261 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4321 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4381 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4441 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctgctcgga tctgttggac
     4501 cggacagtag tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     4561 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     4621 cttagacaac ttaataacac attgcggacg tttttaatgt actggggttg aacactctgt
     4681 gccgaattcg gatccggaga gcggagaatt aagggagtca cgttatgacc cccgccgatg
     4741 acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact
     4801 cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc
     4861 gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca
     4921 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcatactg
     4981 tatttttaca acaattacca acaacaacaa acaacaaaca acattacaat tactatttac
     5041 aattacaatg tctcctgaaa gaaggcctgc tgatattaga agggctactg aggctgatat
     5101 gcctgctgtg tgcactattg tgaatcatta cattgagact tctactgtga atttcaggac
     5161 tgagcctcaa gaaccacaag agtggactga tgatcttgtg aggcttagag agaggtatcc
     5221 ttggcttgtt gctgaagtgg atggtgaagt ggctggtatt gcttatgctg gtccttggaa
     5281 ggctaggaat gcttacgatt ggactgctga gtctactgtt tacgtgtctc ctaggcatca
     5341 aagaactggt cttggatcta ctctttacac tcatcttttg aagtctttgg aggctcaggg
     5401 tttcaagtct gtggttgctg tgattggact tcctaatgat ccttctgtga ggatgcatga
     5461 ggctcttggt tatgctccta ggggaatgct tagagctgct ggtttcaaac atggaaattg
     5521 gcatgatgtg ggattctggc agcttgattt ctctcttcct gttcctccta gacctgttct
     5581 tcctgtgact gagatttgag cttgtcctgc tttaatgaga tatgcgagaa gcctatgatc
     5641 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
     5701 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
     5761 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
     5821 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
     5881 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
     5941 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
     6001 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
     6061 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
     6121 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
     6181 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
     6241 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
     6301 tcgaggctca gcaggaccgc tgcaagaatt caagcttgga gctcaacttt tgattcgcta
     6361 tttgcagtgc acctgtggcg ttcatcacat cttttgtgac actgtttgca ctggtcattg
     6421 ctattacaaa ggaccttcct gatgttgaag gagatcgaaa gtaagtaact gcacgcataa
     6481 ccattttctt tccgctcttt ggctcaatcc atttgacagt caaagacaat gtttaaccag
     6541 ctccgtttga tatattgtct ttatgtgttt gttcaagcat gtttagttaa tcatgccttt
     6601 gattgatctt gaataggttc caaatatcaa ccctggcaac aaaacttgga gtgagaaaca
     6661 ttgcattcct cggttctgga cttctgctag taaattatgt ttcagccata tcactagctt
     6721 tctacatgcc tcaggtgaat tcatctattt ccgtcttaac tatttcggtt aattaaagca
     6781 cgaacaccat tactgcatgt agaagcttga taaactatcg ccaccaattt atttttgttg
     6841 cgatattgtt actttcctca gtatgcagct ttgaaaagac caaccctctt atcctttaac
     6901 aatgaacagg tttttagagg tagcttgatg attcctgcac atgtgatctt ggcttcaggc
     6961 ttaattttcc aggtaaagca ttatgagata ctcttatatc tcttacatac ttttgagata
     7021 atgcacaaga acttcataac tatatgcttt agtttctgca tttgacactg ccaaattcat
     7081 taatctctaa tatctttgtt gttgatcttt ggtagacatg ggtactagaa aaagcaaact
     7141 acaccaaggt aaaatacttt tgtacaaaca taaactcgtt atcacggaac atcaatggag
     7201 tgtatatcta acggagtgta gaaacatttg attattgcag gaagctatct caggatatta
     7261 tcggtttata tggaatctct tctacgcaga gtatctgtta ttccccttcc tctagctttc
     7321 aatttcatgg tgaggatatg cagttttctt tgtatatcat tcttcttctt ctttgtagct
     7381 tggagtcaaa atcggttcct tcatgtacat acatcaagga tatgtccttc tgaattttta
     7441 tatcttgcaa taaaaatgct tgtaccaatt gaaacaccag ctttttgagt tctatgatca
     7501 ctgacttggt tctaaccaaa aaaaaaaaaa tgtttaattt acatatctaa aagtaggttt
     7561 agggaaacct aaacagtaaa atatttgtat attattcgaa tttcactcat cataaaaact
     7621 taaattgcac cataaaattt tgttttacta ttaatgatgt aatttgtgta acttaagata
     7681 aaaataatat tccgtaagtt aaccggctaa aaccacgtat aaaccaggga acctgttaaa
     7741 ccggttcttt actggataaa gaaatgaaag cccatgtaga cagctccatt agagcccaaa
     7801 ccctaaattt ctcatctata taaaaggagt gacattaggg tttttgttcg tcctcttaaa
     7861 gcttctcgtt ttctctgccg tctctctcat tcgcgcgacg caaacgatct tcaggtgatc
     7921 ttctttctcc aaatcctctc tcataactct gatttcgtac ttgtgtattt gagctcacgc
     7981 tctgtttctc tcaccacagc aatggcttct agcccaccga agaagaagcg gaaggtcagc
     8041 tggaaaatgg acaagaagta cagcattgga cttgatattg gtacgaactc agttgggtgg
     8101 gccgttatca ccgatgaata caaggtacct tcgaagaaat ttaaagtgct gggcaacaca
     8161 gataggcaca gcattaagaa gaacttgatc ggagctctgc tctttgactc tggagaaacc
     8221 gcggaggcga caaggcttaa acgtactgcg aggagaaggt acactcgcag gaagaacaga
     8281 atctgttatc tccaagagat ctttagcaac gagatggcga aggtaaggat ttttatgata
     8341 tagtatgctt atgtattttg tactgaaagc atatcctgct tcattgggat attactgaaa
     8401 gcatttaact acatgtaaac tcacttgatg atcaataaac ttgattttgc aggttgacga
     8461 ctcgttcttc catcgcctcg aggaatcctt cctggtagag gaagataaga aacacgagcg
     8521 tcaccccatc tttgggaata ttgttgacga agtagcctat catgaaaagt atccgactat
     8581 ataccacctt cgcaagaagc tggtggactc aaccgataag gcagaccttc ggctcatata
     8641 cctggctctc gcgcacatga taaagtttcg tggccatttc ttgatcgaag gggacctcaa
     8701 cccggataac tccgatgtgg ataaactgtt cattcagctc gtccaaacct acaatcagct
     8761 gttcgaggag aaccccatca atgcatcagg taacattcct tagttacctt tcttttcttt
     8821 ttccatcata agtttataga ttgtacatgc tttgagattt ttctttgcaa acaatctcag
     8881 gtgtcgacgc caaggcaata ctgtctgcca gactttcgaa gtccagacgg cttgagaatc
     8941 tgatcgctca attgccaggc gagaagaaga acggcttgtt cgggaatctg attgcactgt
     9001 ctctgggcct cacccctaac ttcaaaagca actttgacct cgccgaggac gcgaagctgc
     9061 agctgtcaaa ggatacatac gatgatgatc tggacaatct gctcgcccaa ataggtgctc
     9121 ttgaaattgg aactcttctt ttgttgtcta aacctatcaa tttctttgcg gaaatttatt
     9181 tgaagctgta gagttaaaat tgagtctttt aaacttttgt aggtgatcag tatgccgacc
     9241 tgttcttggc tgccaagaat ctgtcagacg ctatcttgct cagtgacatt ctgcgggtca
     9301 acacggagat aaccaaagcg ccacttagcg cctccatgat caagaggtac gacgagcatc
     9361 accaggatct gacccttctg aaggctttgg ttcgccagca actccccgag aagtacaagg
     9421 agattttctt tgaccaatcg aagaatggct acgcagggta cattgatgga ggtaagttgt
     9481 tacttatgat tgttttcctc tctgctacat gtattttgtt gttcatttct gtaagatata
     9541 agaattgagt tttcctctga tgatattatt aggtgcaagt caggaggaat tctacaaatt
     9601 catcaagcct attctggaaa agatggacgg tacagaggag ctgctcgtta aattgaaccg
     9661 cgaagatttg cttcggaagc agcgtacctt cgacaatggc agcataccgc accagatcca
     9721 cctcggtgag ctgcatgcta tcttgaggag gcaagaggac ttctatccgt tcctgaaaga
     9781 caacagagag aagattgaaa agatcctcac gttccgcatt ccctactatg taggttagta
     9841 tcatatgaag aaatacctag tttcagttga tgaatgctat tttctgacct cagttgttct
     9901 cttttgagaa ttatttcttt tctaatttgc ctgatttttc tattaattca ttaggtccac
     9961 tcgcacgcgg gaactcgcgg tttgcgtgga tgacacgcaa atccgaggag actatcacgc
    10021 cttggaactt cgaagaggtc gtggacaagg gtgcgagtgc acagtccttc atcgaaagga
    10081 tgaccaactt cgataagaat ctcccaaatg agaaagtcct gcccaagcat agtctcctgt
    10141 acgaatactt cacggtctac aacgagctga cgaaggtgaa atatgtgacg gaggggatgc
    10201 gcaaaccggc cttcctgtca ggtaaatcct ggtccacact tttacgataa aaacacaaga
    10261 ttttaaacta tgaactgatc aataatcatt cctaaaagac cacacttttg ttttgtttct
    10321 aaagtaattt ttactgttat aacaggtgag cagaagaagg ccattgtcga tctcttgttc
    10381 aaaaccaatc ggaaggtcac tgtgaaacag cttaaagagg actactttaa gaagatcgaa
    10441 tgctttgatt ctgtggaaat cagcggcgtt gaggataggt tcaatgcctc tcttggcaca
    10501 taccatgacc tgttgaaaat catcaaggac aaggacttcc ttgacaacga ggagaacgag
    10561 gacatcctcg aggacatcgt gctgactctc acgctgtttg aggacagaga aatgatcgag
    10621 gagcgcctta agacttatgc gcatctgttc gatgacaagg tcatgaagca gttgaagagg
    10681 aggagatata caggtaagag gtcaaaaggt ttccgcaatg atccctcttt ttttgtttct
    10741 ctagtttcaa gaatttgggt atatgactaa cttctgagtg ttccttgatg catatttgtg
    10801 atgagacaaa tgtttgttct atgttttagg ttggggaagg ctctccagga agctcatcaa
    10861 cggcatccgc gacaagcaat ccggcaagac tatactggac tttctcaaat ccgacggttt
    10921 tgcgaatcgg aacttcatgc agcttattca cgatgactca ctgaccttca aagaagatat
    10981 ccagaaggcc caagtgtcag gtcagggcga tagccttcac gaacacatag ccaacctggc
    11041 tggatcgcca gctataaaga agggcatact gcagacagtg aaggttgtgg atgagctggt
    11101 gaaggtaagt tctgcatttg gttatgctcc ttgcatttta ggtgttcgtc gcacttccat
    11161 ttccatgaat agctaagatt ttttttctct gcattcattc ttcttgcctc agttctaact
    11221 gtttgtggta tttttgtttt aattattgct acaggtcatg ggccgccata agccggagaa
    11281 catcgtcatc gagatggcga gggaaaacca gacgactcag aaagggcaga agaactcacg
    11341 ggagcgcatg aagcggatag aggaaggcat caaggagctt gggagtcaga ttctgaaaga
    11401 gcacccagtc gaaaatactc aactccagaa cgagaagctg tacctctatt acctccagaa
    11461 tgggagagat atgtacgtcg accaagagct cgacattaac agactctccg actatgatgt
    11521 ggatcacatt gtccctcaat ctttcctgaa ggacgatagt attgacaaca aggtaaagca
    11581 actgtgtttt aatcaatttc ttgtcaggat atatggatta taacttaatt tttgagaaat
    11641 ctgtagtatt tggcgtgaaa tgagtttgct ttttggtttc tcccgtgtta taggtcctta
    11701 cgcgctcaga caagaaccgc ggaaaatccg acaatgtacc cagcgaggag gttgtgaaga
    11761 agatgaagaa ctattggagg cagcttttga atgctaagct cataacccaa cggaaattcg
    11821 acaatctcac gaaggcagaa aggggcggac tgtctgagct cgacaaagcc ggcttcatca
    11881 agcgccagtt ggttgaaact cgtcagatta cgaaacatgt ggcccagata ctcgattcgc
    11941 gtatgaatac gaagtatgat gagaatgaca aacttatcag ggaggtaaag gtaaagtttc
    12001 caactttcct ttaccatatc aaactaaagt tcgaaacttt ttatttgatc aacttcaagg
    12061 ccacccgatc tttctattcc tgattaattt gtgatgaatc catattgact tttgatggtt
    12121 acgcaggtga tcaccctcaa gagcaaactg gttagtgact tccggaagga cttccagttt
    12181 tacaaggttc gcgagatcaa caactaccat catgcccatg acgcctacct gaacgccgtt
    12241 gttggcactg ctctcatcaa gaagtatccg aaactggagt ctgagtttgt gtacggggat
    12301 tacaaggtgt acgacgttag gaagatgatc gcgaagtcag aacaagagat cggcaaggct
    12361 accgcgaaat acttctttta ctcgaatatc atgaacttct tcaagacaga gatcactctg
    12421 gcgaatggtg aaatccggaa gaggcctctg atcgagacaa atggcgaaac aggtctgtct
    12481 ttcctatttc atatgtttaa tcctaggaat ttgatcaatt gattgtatgt atgtcgatcc
    12541 caagactttc ttgttcactt atatcttaac tctctctttg ctgtttcttg caggtgagat
    12601 tgtctgggat aagggcaggg attttgcgac tgtgcgtaag gttctcagca tgccccaagt
    12661 caacatagtc aagaaaacgg aggttcaaac cggtggtttc tccaaggagt ccattctccc
    12721 taagcgcaac tccgacaaac tgattgcgag gaagaaggat tgggatccga agaaatacgg
    12781 aggctttgat agccctaccg tggcatacag cgtactggta gtggccaagg tggagaaggg
    12841 caagagcaag aaactgaaaa gcgtcaagga actgcttgga attaccataa tggaaaggtc
    12901 ctcgttcgag aagaatccga tcgacttcct cgaggctaaa ggtaaaatat tggatgccag
    12961 acgatattct ttcttttgat ttgtaacttt ttcctgtcaa ggtcgataaa ttttattttt
    13021 tttggtaaaa ggtcgataat ttttttttgg agccattatg taattttcct aattaactga
    13081 accaaaatta tacaaaccag gttacaaaga ggtgaagaaa gacctcatta tcaaactgcc
    13141 caagtattcg cttttcgaat tggaaaatgg cagaaaacgc atgctggcat ctgccggaga
    13201 actgcagaag ggcaacgagc tggcattgcc cagtaagtac gtcaacttcc tgtacttggc
    13261 ctcacactat gagaagctga aggggtcacc agaggacaac gagcagaagc agttgtttgt
    13321 cgagcagcac aagcactatc ttgatgagat catagagcag atcagcgaat tttccaagcg
    13381 ggtcattctt gcagacgcta acctcgataa ggtaaggact tctcatgaat attagtggca
    13441 gattagtgtt gttaaagtct ttggttagat aatcgatgcc tcctaattgt ccatgtttta
    13501 ctggttttct acaattaaag gtgctttccg cgtacaacaa gcacagagat aagccgataa
    13561 gggaacaagc ggaaaacatc atccacctgt tcacactgac caatctggga gccccagcag
    13621 cctttaagta cttcgatacc actatcgaca gaaagcgcta cacatcaacc aaggaagtgt
    13681 tggacgctac ccttattcac caatctatta cagggctcta tgagacaagg atagatctgt
    13741 cgcagttggg tggtgactct agggctgacc caaagaagaa gcgtaaagtc tgagcttgat
    13801 cctctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
    13861 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
    13921 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
    13981 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
    14041 gcgcgcggtg tcatctatgt tactagatcg acgctactag aattcgagct cggaggccca
    14101 tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag
    14161 tttatataca gctagagtcg aagtagtgat tgagcgaatg caagaaacca aggttttaga
    14221 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
    14281 gtcggtgctt ttttttcgct ttacgaattc ccatggggag ttctagaatg tcgcggaaca
    14341 aattttaaaa ctaaatccta aatttttcta attttgttgc caatagtgga tatgtgggcc
    14401 gtatagaagg aatctattga aggcccaaac ccatactgac gagcccaaag gttcgttttg
    14461 cgttttatgt ttcggttcga tgccaacgcc acattctgag ctaggcaaaa aacaaacgtg
    14521 tctttgaata gactcctctc gttaacacat gcagcggctg catggtgacg ccattaacac
    14581 gtggcctaca attgcatgat gtctccattg acacgtgact tctcgtctcc tttcttaata
    14641 tatctaacaa acactcctac ctcttccaaa atatatacac atctttttga tcaatctctc
    14701 attcaaaatc tcattctctc tagtaaacaa gaacaaaaaa atggcggata cagctagagg
    14761 aacccatcac gatatcatcg gcagagatca gtacccgatg atgggccgag atcgtgacca
    14821 gtaccagatg tccggacgag gatctgacta ctccaagtct aggcagattg ctaaagctgc
    14881 aactgctgtc acagctggtg gttccctcct tgttctctcc agccttaccc ttgttggaac
    14941 tgtcatagct ttgactgttg caacacctct gctcgttatc ttcagcccaa tccttgtccc
    15001 ggctctcatc acagttgcac tcctcatcac cggttttctt tcctctggag ggtttggcat
    15061 tgccgctata accgttttct cttggattta caagtaagca cacatttatc atcttacttc
    15121 ataattttgt gcaatatgtg catgcatgtg ttgagccagt agctttggat caattttttt
    15181 ggtcgaataa caaatgtaac aataagaaat tgcaaattct agggaacatt tggttaacta
    15241 aatacgaaat ttgacctagc tagcttgaat gtgtctgtgt atatcatcta tataggtaaa
    15301 atgcttggta tgatacctat tgattgtgaa taggtacgca acgggagagc acccacaggg
    15361 atcagacaag ttggacagtg caaggatgaa gttgggaagc aaagctcagg atctgaaaga
    15421 cagagctcag tactacggac agcaacatac tggtggggaa catgaccgtg accgtactcg
    15481 tggtggccag cacactacta tgagcgagct gattaaggag aacatgcaca tgaagctgta
    15541 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
    15601 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
    15661 cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca
    15721 gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
    15781 cacatacgaa gatgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
    15841 cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca
    15901 gaagaaaaca ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct
    15961 ggaaggcaga agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt
    16021 caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta
    16081 tgtggaccac agactggaaa gaatcaagga ggccgacaaa gaaacctacg tcgagcagca
    16141 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca agtgagctta
    16201 ccccactgat gtcatcgtca tagtccaata actccaatgt cggggagtta gtttatgagg
    16261 aataaagtgt ttagaatttg atcaggggga gataataaaa gccgagtttg aatctttttg
    16321 ttataagtaa tgtttatgtg tgtttctata tgttgtcaaa tggtaccatg ttttttttcc
    16381 tctctttttg taacttgcaa gtgttgtgtt gtactttatt tggcttcttt gtaagttggt
    16441 aacggtggtc tatatatgga aaaggtcttg ttttgttaaa cttatgttag ttaactggat
    16501 tcgtctttaa ccacaaaaag ttttcaataa gctacaaatt tagacacgca agccgatgca
    16561 gtcattagta catatattta ttgcaagtga ttacatggca acccaaactt caaaaacagt
    16621 aggttgctcc atttagtcgc tcagagagga tgcacatgtg accga
//
