LOCUS       sequence-309575-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for AcpS phosphopantetheinyl transferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Zhou Z, Cironi P, Lin AJ, Xu Y, Hrvatin S, Golan DE, Silver PA, 
            Walsh CT, Yin J
  TITLE     Genetically encoded short peptide tags for orthogonal protein 
            labeling by Sfp and AcpS phosphopantetheinyl transferases.
  JOURNAL   ACS Chem Biol. 2007 May 22;2(5):337-46. doi: 10.1021/cb700054k. Epub
            2007 Apr 27.
  PUBMED    17465518
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb700054k";
            journalName: "ACS Chem Biol"; date: "2007-05-22- 22"; volume: "2"; 
            issue: "5"; pages: "337-46"
COMMENT     Sequence Label: pET-AspS
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             476..493
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(546..564)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      560..607
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(731..750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gtgccgcgcg gcagccatat ggcaatatta ggtttaggca cggatattgt
      121 ggagatcgct cgcatcgaag cggtgatcgc ccgatccggt gatcgcctgg cacgccgcgt
      181 attaagcgat aacgaatggg ctatctggaa aacgcaccac cagccggtgc gttttctggc
      241 gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga tccgcaatgg
      301 tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac ggctacggct
      361 atggggcgag gcattaaaac tggcggaaaa gctgggcgtt gcaaatatgc atgtaacgct
      421 ggcagatgag cggcactatg cttgtgccac ggtaattatt gaaagttaac tcgagcacca
      481 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      541 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      601 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      661 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      721 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      781 cg
//
