LOCUS       sequence-30958-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K
  TITLE     Consideration about negative controls for LC3 and expression vectors
            for four colored fluorescent protein-LC3 negative controls.
  JOURNAL   Autophagy. 2008 Jan 1. 4(1):131-4.
  PUBMED    18000393
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2008 Jan 1. 4(1):131-4."
COMMENT     Sequence Label: pEX-YFP-hLC3WT
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..449)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     CDS             complement(465..716)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(507..528)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggccncacc agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattgagctc
       61 ccgggctcga cttacactga caatttcatc ccgaacgtct cctgggaggc gtagaccatg
      121 tacaggaatc catcttcatc tttctcactc tcatacacct ctgagattgg tgtggagacg
      181 ctgaccatgc tgtgtccgtt caccaacagg aagaaggcct gattagcatt gagctgtaag
      241 cgccttctaa ttatcttgat gagctcactc atgttgacat ggtcaggtac aaggaacttt
      301 gttttatcca gaacaggaag ctgcttctca cccttgtatc gttctattat caccgggatt
      361 ttggttggat gctgctctcg aataagtcgg acatcttcta ctctttgttc gaaggtgcgg
      421 cgctgcttga aggtcttctc cgacggcata gatctgagtc cggacttgta cagctcgtcc
      481 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      541 tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc
      601 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
      661 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
      721 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccan gatgttgccg
      781 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      841 ttcacctcgg cgcgggtctt gtagttgccg tcg
//
