LOCUS       sequence-309582-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for Sfp phosphopantetheinyl transferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Zhou Z, Cironi P, Lin AJ, Xu Y, Hrvatin S, Golan DE, Silver PA, 
            Walsh CT, Yin J
  TITLE     Genetically encoded short peptide tags for orthogonal protein 
            labeling by Sfp and AcpS phosphopantetheinyl transferases.
  JOURNAL   ACS Chem Biol. 2007 May 22;2(5):337-46. doi: 10.1021/cb700054k. Epub
            2007 Apr 27.
  PUBMED    17465518
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb700054k";
            journalName: "ACS Chem Biol"; date: "2007-05-22- 22"; volume: "2"; 
            issue: "5"; pages: "337-46"
COMMENT     Sequence Label: pET-Sfp
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             716..733
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tatacatatg aagatttacg gaatttatat
       61 ggaccgcccg ctttcacagg aagaaaatga acggttcatg actttcatat cacctgaaaa
      121 acgggagaaa tgccggagat tttatcataa agaagatgct caccgcaccc tgctgggaga
      181 tgtgctcgtt cgctcagtca taagcaggca gtatcagttg gacaaatccg atatccgctt
      241 tagcacgcag gaatacggga agccgtgcat ccctgatctt cccgacgctc atttcaacat
      301 ttctcactcc ggccgctggg tcattggtgc gtttgattca cagccgatcg gcatagatat
      361 cgaaaaaacg aaaccgatca gccttgagat cgccaagcgc ttcttttcaa aaacagagta
      421 cagcgacctt ttagcaaaag acaaggacga gcagacagac tatttttatc atctatggtc
      481 aatgaaagaa agctttatca aacaggaagg caaaggctta tcgcttccgc ttgattcctt
      541 ttcagtgcgc ctgcatcagg acggacaagt atccattgag cttccggaca gccattcccc
      601 atgctatatc aaaacgtatg aggtcgatcc cggctacaaa atggctgtat gcgccgcaca
      661 ccctgatttc cccgaggata tcacaatggt ctcgtacgaa gagcttttac tcgagcacca
      721 ccaccaccac cactgagatc cggctgctaa caaagcccga
//
