LOCUS       sequence-309585-        3326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1822
FEATURES             Location/Qualifiers
     source          1..3326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1567..1586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1568..1586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1591..1607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1591..1607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1709..1728)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1720..2529
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1787..1806)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2676..3264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3165..3184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgctc ttcggcgggt ggcggtggat
      601 cgggaggagg aggttcgggt ggcggaggca gtggaggtgg cggaagtggc ggcggtggtt
      661 caggaggagg cggatccatg agtaaaggag aagaattgtt cactggagtt gtcccaatcc
      721 tcgtcgagct cgacggagac gtcaacggac acaagttctc cgtctccgga gagggagagg
      781 gagacgccac ctacggaaag ctcaccctca agttcatctg caccaccgga aagctcccag
      841 tcccatggcc aaccctcgtc accaccttct gctacggagt ccaatgcttc tcccgttacc
      901 cagaccacat gaagcgtcac gacttcttca agtccgccat gccagaggga tacgtccaag
      961 agcgtaccat cttcttcaag gtaagtttaa acatatatat actaactact gattatttaa
     1021 attttcagga cgacggaaac tacaagaccc gtgccgaggt caagttcgag ggagacaccc
     1081 tcgtcaaccg tatcgagctc aaggtaagtt taaacagttc ggtactaact aaccatacat
     1141 atttaaattt tcagggaatc gacttcaagg aggacggaaa catcctcgga cacaagctcg
     1201 agtacaacta caactcccac aacgtctaca tcatggccga caagcaaaag aacggaatca
     1261 aggtcaactt caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1321 ttcagatccg tcacaacatc gaggacggat ccgtccaact cgccgaccac taccaacaaa
     1381 acaccccaat cggagacgga ccagtcctcc tcccagacaa ccactacctc tccacccaat
     1441 ccgccctctc caaggaccca aacgagaagc gtgaccacat ggtcctcctc gagttcgtca
     1501 ccgccgccgg aatcacccac ggaatggacg agctctacaa gcgaagagcg ccatccagct
     1561 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1621 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1681 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1741 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1801 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1861 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1921 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1981 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2041 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2101 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2161 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2221 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2281 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2341 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2401 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2461 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2521 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2581 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2641 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2821 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3061 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3301 ttttgctggc cttttgctca catgtt
//
