LOCUS       sequence-309587-        9132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1820
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1460..1493)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1503..1532
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3349..3672)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5590..5614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5617..6642
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5617..6642
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6643..6667)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7020..7053)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7078..7098
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7120..7251
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7279..7344
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7373..7392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7374..7392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7397..7413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7397..7413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7515..7534)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7526..8335
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7593..7612)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8482..9070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8971..8990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgctc ttcgatgagt aaaggagaag
      601 aattgttcac tggagttgtc ccaatcctcg tcgagctcga cggagacgtc aacggacaca
      661 agttctccgt ctccggagag ggagagggag acgccaccta cggaaagctc accctcaagt
      721 tcatctgcac caccggaaag ctcccagtcc catggccaac cctcgtcacc accttctgct
      781 acggagtcca atgcttctcc cgttacccag accacatgaa gcgtcacgac ttcttcaagt
      841 ccgccatgcc agagggatac gtccaagagc gtaccatctt cttcaaggta agtttaaaca
      901 tatatatact aactactgat tatttaaatt ttcaggacga cggaaactac aagacccgtg
      961 ccgaggtcaa gttcgaggga gacaccctcg tcaaccgtat cgagctcaag gtaagtttaa
     1021 acagttcggt actaactaac catacatatt taaattttca gggaatcgac ttcaaggagg
     1081 acggaaacat cctcggacac aagctcgagt acaactacaa ctcccacaac gtctacatca
     1141 tggccgacaa gcaaaagaac ggaatcaagg tcaacttcaa ggtaagttta aacatgattt
     1201 tactaactaa ctaatctgat ttaaattttc agatccgtca caacatcgag gacggatccg
     1261 tccaactcgc cgaccactac caacaaaaca ccccaatcgg agacggacca gtcctcctcc
     1321 cagacaacca ctacctctcc acccaatccg ccctctccaa ggacccaaac gagaagcgtg
     1381 accacatggt cctcctcgag ttcgtcaccg ccgccggaat cacccacgga atggacgagc
     1441 tctacaaggt aagtttaaaa taacttcgta tagcatacat tatacgaagt tattttcagg
     1501 gagagcaaaa gcttatctcc gaggaggacc tctaaggatg atcgacgcca acgtcgttga
     1561 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     1621 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     1681 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     1741 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     1801 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     1861 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aactagctgc
     1921 cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa ctttattcag tcattcttct
     1981 ctggttcatt tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc ggccacattc
     2041 ctcacctaca tccgtactcc ttatctcccg ttcttatcca gtcttttatc gtctctgcgt
     2101 ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga aaacttgcgg
     2161 aaagatttcc taataaaagg agacgaatcc ggtaggaaat cgacaagtct ctgcttctcg
     2221 aagactaggt gagttacatt tctttggccg aattattttg aaatcctaaa tttgataatt
     2281 tcagagatgt ctgtaaaact tgcgtgttat gtgacggctt cggtcactgt cgccactctt
     2341 atggtatgct tcatgaccat gtcaaccatc tactcggagg ttgatggatt cagagagaag
     2401 cttgataccg agatgaatgt gttcagagtg agttaaatta tattttgaat tttaaataat
     2461 ttttaatttt ctagcaatct accaatggat tgtggaagga catagttgtc atcggaagat
     2521 ctagcaagcg tgtccgttgt caatatgaag agaccaacgc taccccaact ccacatgctg
     2581 atggatcccc atctgctcca ccaggtcaac caccagcagt tccaccagtc ttcaaccagc
     2641 caaagactcc aaatggagcc aatggaaatg gaccaacctg caactgcaat gctgataaca
     2701 agtgcccagc tggaccatcc ggaccaaagg gagttccagg agttccagga ctcgacggag
     2761 ttccaggact tgacggtgtt ccaggagttg gagctgatga tatcgctcca caacgcgagt
     2821 ctgtcggatg cttcacttgc ccacaaggac cagttggacc accaggagct cttggaagac
     2881 caggaccacg tggacttcca ggaccaagag gacaaaatgg aaacccagga agagatggac
     2941 aaccaggaca tccaggagag caaggatcat ccggtcaaat cggaaagatc ggagagccag
     3001 gaccaccagg agagaaggga cgcgacgccg agcatccaat cggaagacca ggaccaaagg
     3061 gaccaagagg agatcaagga ccaacaggac cagctggaca gaacggtctt cacggaccac
     3121 caggagagcc aggaaccgtt ggaccagaag gaccatctgg aaagcaagga cgtcaaggac
     3181 cagacggaac ccagggagag actggaccag acggaagacc aggaaaggat gccgagtact
     3241 gccagtgccc agacaagtct ccaccatcag aggctgtcaa cgccaaccgt ggatacagaa
     3301 atatctaaat tgttggtgtt ttctaataaa atatttgaga ttcagtgaga cttttttctt
     3361 ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt
     3421 tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaatg gaattgaaga
     3481 gagattgtga aagtacatca gacagaatca cattttgata gcattcactt cactcagatg
     3541 caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt
     3601 agcgagagaa ttttttgaca aaaagaaaga agagtgatag agaagaaggg aatgcttgaa
     3661 aggattttgc atttatctta atcaccgtct tcgagaagac gaaccatggc gccagtttca
     3721 gaatccaagt ttctaatata gttcatcaca atgttgacgt tggtccaacc tccggcttgc
     3781 ataatctctg ggatggaaac tccagcgcga gccatgtcac gggcagctcc gacacgggcg
     3841 gaatgtccag accaagccaa gtaacgttgt ccggaatcat ccttggctcc gtagatgaga
     3901 cggtgggtag cttcgaagat tcctgaaaat ttattttcag cttattctta gctaatatgt
     3961 tgtttaaact taccttcaag agctcgagtc gagagttgtg atgttgcact cggggcagca
     4021 actccgttct tgcgaacacg acagaacagg tagttgtttg gatcatcggc gactccggaa
     4081 accgaaatcc agcgctcaac gagcttggta actccaaggg agagcgcttt ttcgactccg
     4141 gcagtcgaga ccagcgtttt tgtacgtccg atgtggatga gcattcttcc tccgtctgtg
     4201 cgactaatat ccttgacgcg aatgcgggca atttcggcga tacggagaag tgtgttgtaa
     4261 gcaattccga ggaaggcgag gtttcggatg tcctggcaac gatcggaatt ttccataaga
     4321 gaacggacct gatcgaagtc ggttctgaaa atttcgtcaa gtcttaggat tgaatacatt
     4381 aatatttaaa cttacctttc gaaggcgagg gcttgcttgg ctctctctcc agcatcaaca
     4441 ttttccttgc ggatacgtct cattacgaga gatacggcat tagaatctga tgggcgtgga
     4501 agtccagatc ggcgatggag catattgagc tgtccgagat gttgttggat cgtcttcacc
     4561 gccaatccac gagcttggag gtagagcaag tagtcacgaa cgtcttctgg ctcggctggg
     4621 aaccacttgc ggttgttaag cttgcaccag gcagcccaag aacggcacac tgagagaagc
     4681 atcttccagg tgtgctcgga gaaggcttgt ctgtcacgga acatatccat gaggttcttg
     4741 cgaacttcat cacttgtcgc gtcaactgga agtgctggaa gattttgatg aacagtgaga
     4801 agatttgaca tattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc
     4861 tgagcccgct ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc
     4921 tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta
     4981 tctgggtctc tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt
     5041 ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa
     5101 aataaatacc gaacaacatt tgctctaatt gtgaaattag aaatcttcaa actataatca
     5161 tgtcacttta ccactatttc cgtccagctc aacgttccgt ttttggtgat catttttgct
     5221 ttcgtcgtaa atctacacac gcgtctcttc cgtgcgagag tccaagccag cagccaaatt
     5281 cgttgactga gtattcaacg tttatacgtt gtcggcaacg agaaatagga aaatgcatcg
     5341 ggaaatgttc ttttttcgat tttttccaag gttttgacaa attttaccac gaattttgct
     5401 atgttttcaa ttaaaaaata tgttattcaa ctgtttctat gaggaaaata aggctttgca
     5461 tgtaattttc ttattcagca taatttttaa ttaatttgaa ttttctgtcc taacgtttat
     5521 tttgttttct tggttatgac tgatctgaaa ttaatttttg aattttaagg taatatgtca
     5581 ggcggtgccg caagtttgta caaaaaagca ggctccatga aaaagcctga actcaccgcg
     5641 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     5701 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     5761 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     5821 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     5881 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     5941 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     6001 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     6061 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     6121 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     6181 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     6241 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     6301 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     6361 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     6421 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     6481 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     6541 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     6601 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agacccagct ttcttgtaca
     6661 aagtgggtcc aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc
     6721 ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag
     6781 tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc
     6841 gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg
     6901 ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa
     6961 aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttaa
     7021 taacttcgta tagcatacat tatacgaagt tattttcagg gtggcggcag cggaggagag
     7081 aatctgtact ttcaatccgg atccggaggt ggcgggatgc ctaaagatcc agccaaacct
     7141 ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa gaacgtgatg
     7201 gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa gggtggtggc
     7261 ggttctggct caggatctga ctacaaagac catgacggtg attataaaga tcatgatatc
     7321 gattacaagg atgacgatga caagacgcga agagcgccat ccagctgata tcccctatag
     7381 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     7441 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     7501 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     7561 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     7621 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     7681 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     7741 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     7801 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     7861 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     7921 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     7981 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     8041 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     8101 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     8161 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     8221 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     8281 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     8341 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     8401 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     8461 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8521 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8581 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8641 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8701 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8761 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8821 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8881 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8941 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9001 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9061 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9121 tgctcacatg tt
//
