LOCUS       sequence-30959-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K
  TITLE     Consideration about negative controls for LC3 and expression vectors
            for four colored fluorescent protein-LC3 negative controls.
  JOURNAL   Autophagy. 2008 Jan 1. 4(1):131-4.
  PUBMED    18000393
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2008 Jan 1. 4(1):131-4."
COMMENT     Sequence Label: pEX-YFP-hLC3-Delta-G
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..437)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFMKLSV"
     CDS             complement(453..704)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(495..516)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     844..863
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagccaccac cttctgatag gcagcctgca cctgaggagt gaattgagct cccgggctcg
       61 acttacactg acaatttcat gaacgtctcc tgggaggcgt agaccatgta caggaatcca
      121 tcttcatctt tctcactctc atacacctct gagattggtg tggagacgct gaccatgctg
      181 tgtccgttca ccaacaggaa gaaggcctga ttagcattga gctgtaagcg ccttctaatt
      241 atcttgatga gctcactcat gttgacatgg tcaggtacaa ggaactttgt tttatccaga
      301 acaggaagct gcttctcacc cttgtatcgt tctattatca ccgggatttt ggttggatgc
      361 tgctctcgaa taagtcggac atcttctact ctttgttcga aggtgcggcg ctgcttgaag
      421 gtcttctccg acggcataga tctgagtccg gacttgtaca gctcgtccat gccgagagtg
      481 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      541 ttgctcaggg cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      601 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      661 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      721 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      781 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      841 cgggtcttgt agttgccgtc gtccttga
//
