LOCUS       sequence-30961-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K
  TITLE     Consideration about negative controls for LC3 and expression vectors
            for four colored fluorescent protein-LC3 negative controls.
  JOURNAL   Autophagy. 2008 Jan 1. 4(1):131-4.
  PUBMED    18000393
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2008 Jan 1. 4(1):131-4."
COMMENT     Sequence Label: pEX- HcRed -hLC3-Delta-G
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..716
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 taccgatatc gctagcgcta ccggtcgcca ccatggtgag cggcctgctg aaggagagta
       61 tgcgcatcaa gatgtacatg gagggcaccg tgaacggcca ctacttcaag tgcgagggcg
      121 agggcgacgg caaccccttc gccggcaccc agagcatgag aatccacgtg accgagggcg
      181 cccccctgcc cttcgccttc gacatcctgg ccccctgctg cgagtacggc agcaggacct
      241 tcgtgcacca caccgccgag atccccgact tcttcaagca gagcttcccc gagggcttca
      301 cctgggagag aaccaccacc tacgaggacg gcggcatcct gaccgcccac caggacacca
      361 gcctggaggg caactgcctg atctacaagg tgaaggtgca cggcaccaac ttccccgccg
      421 acggccccgt gatgaagaac aagagcggcg gctgggagcc cagcaccgag gtggtgtacc
      481 ccgagaacgg cgtgctgtgc ggccggaacg tgatggccct gaaggtgggc gaccggcacc
      541 tgatctgcca ccactacacc agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc
      601 ccggcttcca cttcaccgac atccggctcc agatgctgcg gaagaagaag gacgagtact
      661 tcgagctgta cgaggccagc gtggcccggt acagcgacct gcccgagaag gccaactccg
      721 gactcagatc tatgccgtcg gagaagacct tcaagcagcg ccgcaccttc gaacaaagag
      781 tagaagatgt ccgacttatt cgagagcagc atccaaccaa aatcccggtg ataatagaac
      841 gatacaaggg tgagaagcag cttcctgttc tggataaaca aagttccttg tacctgacca
      901 tgtcaacatg agtgagctca tcaagataat taga
//
