LOCUS       sequence-309613-        8369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retrograde AAV helper vector, carrying rep and modified capsid gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Tervo DG, Hwang BY, Viswanathan S, Gaj T, Lavzin M, Ritola KD, Lindo
            S, Michael S, Kuleshova E, Ojala D, Huang CC, Gerfen CR, Schiller J,
            Dudman JT, Hantman AW, Looger LL, Schaffer DV, Karpova AY
  TITLE     A Designer AAV Variant Permits Efficient Retrograde Access to 
            Projection Neurons.
  JOURNAL   Neuron. 2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 
            10.1016/j.neuron.2016.09.021.
  PUBMED    27720486
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Oct 6. pii: S0896-6273(16)30580-3. doi: 
            10.1016/j.neuron.2016.09.021."
COMMENT     Sequence Label: rAAV2-retro helper
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   44..146
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             4249..4260
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      5186..5699
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5273..5292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5483..5504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6181..6200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6300..6322
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6360..6378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6446..6550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6551..6619,6620..7411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6769..6788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8324..8341
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagaat tcccatcatc aataatatac
       61 cttattttgg attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg
      121 gcgtgggaac ggggcgggtg acgtagtagc tctagaggtc ctgtattaga ggtcacgtga
      181 gtgttttgcg acattttgcg acaccatgtg gtcacgctgg gtatttaagc ccgagtgagc
      241 acgcagggtc tccattttga agcgggaggt ttgaacgcgc agccaccacg gcggggtttt
      301 acgagattgt gattaaggtc cccagcgacc ttgacgagca tctgcccggc atttctgaca
      361 gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc gccagattct gacatggatc
      421 tgaatctgat tgagcaggca cccctgaccg tggccgagaa gctgcagcgc gactttctga
      481 cggaatggcg ccgtgtgagt aaggccccgg aggccctttt ctttgtgcaa tttgagaagg
      541 gagagagcta cttccacatg cacgtgctcg tggaaaccac cggggtgaaa tccatggttt
      601 tgggacgttt cctgagtcag attcgcgaaa aactgattca gagaatttac cgcgggatcg
      661 agccgacttt gccaaactgg ttcgcggtca caaagaccag aaatggcgcc ggaggcggga
      721 acaaggtggt ggatgagtgc tacatcccca attacttgct ccccaaaacc cagcctgagc
      781 tccagtgggc gtggactaat atggaacagt atttaagcgc ctgtttgaat ctcacggagc
      841 gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca gacgcaggag cagaacaaag
      901 agaatcagaa tcccaattct gatgcgccgg tgatcagatc aaaaacttca gccaggtaca
      961 tggagctggt cgggtggctc gtggacaagg ggattacctc ggagaagcag tggatccagg
     1021 aggaccaggc ctcatacatc tccttcaatg cggcctccaa ctcgcggtcc caaatcaagg
     1081 ctgccttgga caatgcggga aagattatga gcctgactaa aaccgccccc gactacctgg
     1141 tgggccagca gcccgtggag gacatttcca gcaatcggat ttataaaatt ttggaactaa
     1201 acgggtacga tccccaatat gcggcttccg tctttctggg atgggccacg aaaaagttcg
     1261 gcaagaggaa caccatctgg ctgtttgggc ctgcaactac cgggaagacc aacatcgcgg
     1321 aggccatagc ccacactgtg cccttctacg ggtgcgtaaa ctggaccaat gagaactttc
     1381 ccttcaacga ctgtgtcgac aagatggtga tctggtggga ggaggggaag atgaccgcca
     1441 aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa ggtgcgcgtg gaccagaaat
     1501 gcaagtcctc ggcccagata gacccgactc ccgtgatcgt cacctccaac accaacatgt
     1561 gcgccgtgat tgacgggaac tcaacgacct tcgaacacca gcagccgttg caagaccgga
     1621 tgttcaaatt tgaactcacc cgccgtctgg atcatgactt tgggaaggtc accaagcagg
     1681 aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt tgaggtggag catgaattct
     1741 acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag tgacgcagat ataagtgagc
     1801 ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc agacgcggaa gcttcgatca
     1861 actacgcaga caggtaccaa aacaaatgtt ctcgtcacgt gggcatgaat ctgatgctgt
     1921 ttccctgcag acaatgcgag agaatgaacc agaattcaaa tatctgcttc actcacggac
     1981 agaaagactg tttagagtgc tttcccgtgt cagaatctca acccgtttct gtcgtcaaaa
     2041 aggcgtatca gaaactgtgc tacattcatc atatcatggg aaaggtgcca gacgcttgca
     2101 ctgcctgcga tctggtcaat gtgggtttgg atgactgcat ctttgaacaa taaatgattt
     2161 aaatcaggta tggctgccga tggttatctt ccagattggc tcgaggacac tctctctgaa
     2221 ggaataagac agtggtggaa gctcaaacct ggcccaccac caccaaagcc cgcagagcgg
     2281 cataaggacg acagcagggg tcttgtgctt cctgggtaca agtacctcgg acccttcaac
     2341 ggactcgaca agggagagcc ggtcaacgag gcagacgccg cggccctcga gcacgacaaa
     2401 gcctacgacc ggcagctcga cagcggagac aacccgtacc tcaagtacaa ccacgccgac
     2461 gcggagtttc aggagcgcct taaagaagat acgtcttttg ggggcaacct cggacgagca
     2521 gtcttccagg cgaaaaagag ggttcttgaa cctctgggcc tggttgagga acctgttaag
     2581 acggctccgg gaaaaaagag gccggtagag cactctcctg tggagccaga ctcctcctcg
     2641 ggaaccggaa aggcgggcca gcagcctgca agaaaaagat tgaattttgg tcagactgga
     2701 gacgcagact cagtacctga cccccagcct ctcggacagc caccagcagc cccctctggt
     2761 ctgggaacta atacgatggc tacaggcagt ggcgcaccaa tggcagacaa taacgagggc
     2821 gccgacggag tgggtaattc ctcgggaaat tggcattgcg attccacatg gatgggcgac
     2881 agagtcatca ccaccagcac ccgaacctgg gccctgccca cctacaacaa ccacctctac
     2941 aaacaaattt ccagccaatc aggagcctcg aacgacaatc actactttgg ctacagcacc
     3001 ccttgggggt attttgactt caacagattc cactgccact tttcaccacg tgactggcaa
     3061 agactcatca acaacaactg gggattccga cccaagagac tcaacttcaa gctctttaac
     3121 attcaagtca aagaggtcac gcagaatgac ggtacgacga cgattgccaa taaccttacc
     3181 agcacggttc aggtgtttac tgactcggag taccagctcc cgtacgtcct cggctcggcg
     3241 catcaaggat gcctcccgcc gttcccagca gacgtcttca tggtgccaca gtatggatac
     3301 ctcaccctga acgacgggag tcaggcagta ggacgctctt cattttactg cctggagtac
     3361 tttccttctc agatgctgcg taccggaaac aactttacct tcagctacac ttttgaggac
     3421 gttcctttcc acagcagcta cgctcacagc cagagtctgg accgtctcat gaatcctctc
     3481 atcgaccagt acctgtatta cttgagcaga acaaacactc caagtggaac caccacgcag
     3541 tcaaggcttc agttttctca ggccggagcg agtgacattc gggaccagtc taggaactgg
     3601 cttcctggac cctgttaccg ccagcagcga gtatcaaaga catctgcgga taacaacaac
     3661 agtgaatact cgtggactgg agctaccaag taccacctca atggcagaga ctctctggtg
     3721 aatccgggcc cggccatggc aagccacaag gacgatgaag aaaagttttt tcctcagagc
     3781 ggggttctca tctttgggaa gcaaggctca gagaaaacaa atgtggacat tgaaaaggtc
     3841 atgattacag acgaagagga aatcaggaca accaatcccg tggctacgga gcagtatggt
     3901 tctgtatcta ccaacctcca gagaggcaac ctagcagacc aagactacac aaaaactgct
     3961 aggcaagcag ctaccgcaga tgtcaacaca caaggcgttc ttccaggcat ggtctggcag
     4021 gacagagatg tgtaccttca ggggcccatc tgggcaaaga ttccacacac ggacggacat
     4081 tttcacccct ctcccctcat gggtggattc ggacttaaac accctcctcc ccagattctc
     4141 atcaagaaca ccccggtacc tgcgaatcct tcgaccacct tcagtgcggc aaagtttgct
     4201 tccttcatca cacagtactc cacgggacag gtcagcgtgg agatcgagtg ggagctgcag
     4261 aaggaaaaca gcaaacgctg gaatcccgaa attcagtaca cttccaacta caacaagtct
     4321 attaatgtgg actttactgt ggacactaat ggcgtgtatt cagagcctcg ccccattggc
     4381 accagatacc tgactcgtaa tctgtaattg cggccgcttg ttaatcaata aaccgtttaa
     4441 ttcgtttcag ttgaactttg gtctctgcgt atttctttct tatctagttt ccatgctcta
     4501 gaggtcctgt attagaggtc acgtgagtgt tttgcgacat tttgcgacac catgtggtca
     4561 cgctgggtat ttaagcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga
     4621 acgcgcagcc accacggcgg ggttttacga gattgtgatt aaggtcccca gcgaccttga
     4681 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga
     4741 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc
     4801 cgagaagctg catcgctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4861 gttgcgcagc ctgaatggcg aatggcgatt ccgttgcaat ggctggcggt aatattgttc
     4921 tggatattac cagcaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta
     4981 ctaatcaaag aagtattgcg acaacggtta atttgcgtga tggacagact cttttactcg
     5041 gtggcctcac tgattataaa aacacttctc aggattctgg cgtaccgttc ctgtctaaaa
     5101 tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat
     5161 acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5401 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5521 atctcggtct attcttttga tttataaggg attttgccga tttcggcata ttggttaaaa
     5581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5641 taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc aaccggggta
     5701 catatgattg acatgctagt tttacgatta ccgttcatcg attctcttgt ttgctccaga
     5761 ctctcaggca atgacctgat agcctttgta gagacctctc aaaaatagct accctctccg
     5821 gcatgaattt atcagctaga acggttgaat atcatattga tggtgatttg actgtctccg
     5881 gcctttctca cccgtttgaa tctttaccta cacattactc aggcattgca tttaaaatat
     5941 atgagggttc taaaaatttt tatccttgcg ttgaaataaa ggcttctccc gcaaaagtat
     6001 tacagggtca taatgttttt ggtacaaccg atttagcttt atgctctgag gctttattgc
     6061 ttaattttgc taattctttg ccttgcctgt atgatttatt ggatgttgga atcgcctgat
     6121 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     6181 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6241 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6301 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     6361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8341 ggaagcggaa gagcgcccaa tacgcaaac
//
