LOCUS       sequence-30963-e         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del 
            Cardayre SB, Keasling JD.
  TITLE     Microbial production of fatty-acid-derived fuels and chemicals from 
            plant biomass.
  JOURNAL   Nature. 2010 Jan 28;463(7280):559-62.
  PUBMED    20111002
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-01-28"; volume: "463(7280)"; pages: "559-62"
COMMENT     Sequence Label: pGB3
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..25)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(29..52)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atttatcgtc gtcgtctttg taatcgcctt tgtcatcatc gtctttatag tcagatcttt
       61 cacgcagacg ggatgggtcc acgattgccc agaaggcagg tttcggcata aagttacgat
      121 caaacagcag aggggcgttg gtgcgcttca ccgggaagcc gttcagccaa gagtatttgt
      181 cagaaatgcc ccagaacacg accgcggaaa caatgtcttt gttttctttc agcgcatcga
      241 acagctcctg ataacgcttt gcctgcaggg tcagaatgta atccggaata gagtcaccat
      301 aatcggaacg gtcgttccag gagtaaatgc tcatgtccag ttcggtgatg atgttgtcca
      361 ggcccaggcc cgcgaacttt ttaatggatt caatgatgcg ttctaccggc ggattataga
      421 tatcaatatg ggtctggtga ccaacaccgt cgataggtac gcctttttcc agcaggtttt
      481 taaccagctc atacaggatg tcacgtttaa ccggatcatc ggtgttatag tcgttgatgt
      541 acagtttgat gtcggaacca ccggcttcac gtgcctg
//
