LOCUS       sequence-309633-       11084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Venus under SERT gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfSERT-0.5k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..11084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    165..259
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(860..862,863..865,866..1579)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(905..926)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1166..1185)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1513..1534
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(1608..2182,2183..2185,2186..2194)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1775..1792)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2002..2021
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2195..2635
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2660..3202
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    3286..3874
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3339..3359)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3757..3768)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4079..4303
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4436..4455)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5477..5494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5648..6236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5728..5747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6407..7198,7199..7267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7030..7049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7268..7372)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7440..7458
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7496..7518)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7618..7637
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8899..8919
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8962..9142
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10524..10757
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    join(11019..11084,1..52)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
       61 ttacagggac agcagagatc cactttgggc ggcctaggga taacagggta atagatcttg
      121 ctagctctta tcatgtctgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc
      181 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
      241 cggagtactg tcctccgagc ggagactcta gagggtcccg ggatacgcgt gggacagaga
      301 ggaattagac aaggctaagc tgaactcaca tcccagcagt cagataaacg catgggtacc
      361 aagtactgtt aacgtcccag gaagaaaaag caggtttcgg gatgggggcg atggggaggt
      421 gttggaggtc aagaggaagc ggcaccagca gacccctatg tgccgttctg ggggaggggg
      481 gcggcagggg aggcgcatac cccgtgcccc gttgtgcagc ctcccccctc ccgcaaagtt
      541 aaagagcagg aaagtcagga ttcctcgctc ggccctgccc taccctgcct gctgctccgc
      601 gctccgctcc tccctgcgag cgtgtgtgtg tcgggggtcc ctcccctcct ggcgctgggg
      661 tcgggcgcgc accccgccca gtagcgcggc ccctcccggg cgagcgcaac cccatccagc
      721 gggagcgccg agccgcggcc gcggggaagc attaagttta ttcgcctcaa agtgacgcaa
      781 aaattcttca agagctcttt ggcggcggct atctagagat cagaccatgt gaggaccggt
      841 gagcggccgc ggatccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      901 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      961 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     1021 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     1081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1141 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1261 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     1321 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     1381 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1441 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     1501 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1561 ggacgagctg tacaagtaag gcgcgcccta gtcggatatc cgtcgagccc ctctccctcc
     1621 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     1681 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     1741 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     1801 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     1861 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     1921 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     1981 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     2041 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
     2101 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     2161 ctttgaaaaa cacgatgata atatggccac aaccatgaag ctactgtctt ctatcgaaca
     2221 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
     2281 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
     2341 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
     2401 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
     2461 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
     2521 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
     2581 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga
     2641 attgggatcc atggtgtttc cttctgggca gatctcaaac caggccctgg ccttagcacc
     2701 gtcctctgcc ccagtccttg cccagaccat ggtcccttcc tcagccatgg tacctctggc
     2761 tcagccccca gctcctgccc cagttctaac cccgggtcct ccccagtccc tgtctgcacc
     2821 tgttccaaag agcacccagg ctggggaagg cacgctgtcg gaagccctgc tgcacctgca
     2881 gtttgatgct gatgaagact tgggggcctt gcttggcaac agcacagacc caggagtgtt
     2941 cacagacctg gcatctgtgg acaactcaga gtttcagcag ctcctgaacc agggtgtgtc
     3001 catgtctcac tccacagctg agcccatgct gatggagtac cctgaagcta taactcgcct
     3061 ggtgacaggg tcccagaggc cccctgaccc agctcccaca cccctgggga cctcggggct
     3121 tcccaatggt ctctccggag atgaagactt ctcctccatt gcggacatgg acttctctgc
     3181 tctgctgagt cagatcagct ccagcggcca atctagactg cagctcgacc tcgagggggg
     3241 gcccggtacc aggtaagtta acatcgaggg atcaagctta tcgataatca acctctggat
     3301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3661 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3841 acgagtcgga tctccctttg ggccgcctcc ccgcatcgtc gactagggat aacagggtaa
     3901 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3961 aaaagggggg actggaaggg ctaattcact cccagggtct ctctggttag accagatctg
     4021 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcata aagctcgact
     4081 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4141 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4201 agtaggtgtc attctattct ggggggtagg gtggggcagg acagcaaggg ggaggattgg
     4261 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4321 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4381 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4441 ttcgctttct tcccttcctt tctcgccacg ttcgccggct gtgcgggaga acggagttct
     4501 attatgactc aaatcagtct ccccaagcat tcggggatca gagtttttaa ggataactta
     4561 gtgtgtaggg ggccagtgag ttggagatga aagcgtaggg agtcgaaggt gtccttttgc
     4621 gccgagtcag ttcctgggtg ggggccacaa gatcggatga gccagtttat caatccgggg
     4681 gtgccagctg atccatggag tgcagggtct gcaaaatatc tcaagcactg attgatctta
     4741 ggttttacaa tagtgatgtt accccaggaa caatttgggg aaggtcagaa tcttgtagcc
     4801 tgtagctgca tgactcctaa accataattt cttttttgtt tttttttttt tatttttgag
     4861 acagggtctc actctgtcac ctaggctgga gtgcagtggt gcaatcacag ctcactgcag
     4921 cctcaacgtc gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag ctgagactac
     4981 aagcgacgcc ccagttaatt tttgtatttt tggtagaggc agcgttttgc cgtgtggccc
     5041 tggctggtct cgaactcctg ggctcaagtg atccagcctc agcctcccaa agtgctggga
     5101 caaccggggc cagtcactgc acctggccct aaaccataat ttctaatctt ttggctaatt
     5161 tgttagtcct acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt tcgggaaagg
     5221 gctgttactg tctttgtttc aaactataaa ctaagttcct cctaaactta gttcggccta
     5281 cacccaggaa tgaacaagga gagcttggag gttagaagca cgatggaatt ggttaggtca
     5341 gatctctttc actgtctgag ttataatttt gcaatggtgg ttcaaagact gcccgcttct
     5401 gacaccagtc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7381 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     7441 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     7501 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     7561 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     7621 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     7681 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     7741 ggcgatcggt gcgggcctct tcgctattac gccaggggag gcagagattg cagtaagctg
     7801 agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa
     7861 taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt
     7921 ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct
     7981 ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca
     8041 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac
     8101 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata
     8161 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg
     8221 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag
     8281 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc
     8341 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg
     8401 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca
     8461 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg
     8521 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta
     8581 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta
     8641 tatacagggt tcagtactat cgcatttcag gcccgagagc tgcatccgga gtatctagat
     8701 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     8761 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     8821 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     8881 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt actctagaag gtctatataa
     8941 gcagagctcg tttagtgaac cgggtctctc tggttagacc agatctgagc ctgggagctc
     9001 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgctcaaa
     9061 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9121 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc
     9181 cagaggagat ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9241 ggcggcgact gcagagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9301 ggtgcgagag cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta
     9361 aggccagggg gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta
     9421 gaacgattcg cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg
     9481 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9541 atagcagtcc tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta
     9601 gataagatag aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac
     9661 acaggaaaca acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa
     9721 atggtacatc aggccatatc acctagaact ttaaatgcat gggtaaaagt agtagaagag
     9781 aaggctttca gcccagaagt aatacccatg ttttcagcat tatcagaagg agccacccca
     9841 caagatttaa ataccatgct aaacacagtg gggggacatc aagcagccat gcaaatgtta
     9901 aaagagacca tcaatgagga agctgcagaa tgggatagat tgcatccagt gcatgcaggg
     9961 cctattgcac caggccagat gagagaacca aggggaagtg acatagcagg aactactagc
    10021 aaattaagag aacaatttgg aaataataaa acaataatct ttaagcaatc ctcaggaggg
    10081 gacccagaaa ttgtaacgca cagttttaat tgtggagggg aatttttcta ctgtaattca
    10141 acacaactgt ttaatagtac ttggtttaat agtacttgga gtactgaagg gtcaaataac
    10201 actgaaggaa gtgacacaat cacactccca tgcagaataa aacaatttat aaacatgtgg
    10261 caggaagtag gaaaagcaat gtatgcccct cccatcagtg gacaaattag atgttcatca
    10321 aatattactg ggctgctatt aacaagagat ggtggtaata acaacaatgg gtccgagatc
    10381 ttcagacctg gaggaggcga tatgagggac aattggagaa gtgaattata taaatataaa
    10441 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
    10501 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
    10561 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctgat
    10621 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
    10681 ctcacagtct ggggcatcaa acagctccag gcaagaatcc tggctgtgga aagataccta
    10741 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
    10801 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa taacatgacc
    10861 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
    10921 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggct
    10981 tgcggccgga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    11041 ggggggtaca gtgcagggga aagaatagta gacataatag caac
//
