LOCUS       sequence-309650-        7305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wrmScarlet1-10 in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Goudeau J, Sharp CS, Paw J, Savy L, Leonetti MD, York AG, Updike DL,
            Kenyon C, Ingaramo M
  TITLE     Split-wrmScarlet and split-sfGFP: tools for faster, easier 
            fluorescent labeling of endogenous proteins in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Apr 15;217(4). pii: 6126424. doi: 
            10.1093/genetics/iyab014.
  PUBMED    33693628
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-04-15"; volume: "217(4). pii: 6126424. doi"; pages: " 
            10.1093/genetics/iyab014"
COMMENT     Sequence Label: pRS423-GPD-wrmScarlet1-10-CYC1 terminator
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..644
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     538..561
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      1295..1542
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1311..1329)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1602..1622)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1602..1620)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1641..1657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1641..1657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1654..1676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1665..1681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1689..1695,1696..1713,1714..1719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1734..1755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1872..1889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2043..2631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2123..2142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2802..3593,3594..3662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3425..3444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3663..3767)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3794..5136
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(4757..4804)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5177..5195
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5233..5255)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5355..5374
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5521..5708
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             5709..6371
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(6631..7086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6768..6789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6980..6999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7210..7232
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7224..7241
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7225..7241
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7251..7270
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7251..7269
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcattatcaa tactcgccat ttcaaagaat acgtaaataa ttaatagtag tgattttcct
       61 aactttattt agtcaaaaaa ttagcctttt aattctgctg taacccgtac atgcccaaaa
      121 tagggggcgg gttacacaga atatataaca tcgtaggtgt ctgggtgaac agtttattcc
      181 tggcatccac taaatataat ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa
      241 tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc attctcttag
      301 cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct caatggagtg
      361 atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat gtatctatct
      421 cattttctta caccttctat taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa
      481 aggttgaaac cagttccctg aaattattcc cctacttgac taataagtat ataaagacgg
      541 taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc tacttttata
      601 gttagtcttt tttttagttt taaaacacca gaacttagtt tcgacggatt ctagaactag
      661 tatggtctct aagggtgaag ccgtaattaa ggagttcatg agattcaaag tccacatgga
      721 aggttcaatg aacggacatg agttcgagat agaaggagag ggggagggga gaccttatga
      781 ggggacccag acggccaagt tgaaagtgac gaagggaggt ccattgccct tttcctggga
      841 catactgagt ccccaattca tgtatggaag ccgtgcgttt attaagcacc ccgctgatat
      901 ccctgactac tataaacaat ctttcccgga aggcttcaag tgggaaagag tgatgaattt
      961 cgaggacggc ggggcggtta cggtgactca ggacacttcc cttgaagacg ggaccttaat
     1021 ttataaagta aaattgaggg ggacgaactt tccccccgac ggtccagtaa tgcaaaaaaa
     1081 aacaatgggc tgggaagcct ctactgaaag gttgtatccc gaagacggcg tgttaaaggg
     1141 agatattacg atggctttaa ggctaaagga cggggggagg tatctggcgg atacgtctac
     1201 cacatacaag gccaagaaac cggtccaaat gccgggcgct tacctggtcg acaggaaact
     1261 tgatataaca agtcataatg aatattaact cgagtcatgt aattagttat gtcacgctta
     1321 cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa
     1381 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
     1441 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
     1501 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccggta cccaattctt
     1561 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     1621 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1681 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1741 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1801 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3781 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     3841 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3901 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     3961 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     4021 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     4081 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     4141 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     4201 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     4261 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     4321 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     4381 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     4441 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     4501 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     4561 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     4621 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     4681 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     4741 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     4801 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     4861 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     4921 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     4981 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     5041 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     5101 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     5161 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     5221 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     5281 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5341 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata aattcccgtt
     5401 ttaagagctt ggtgagcgct aggagtcact gccaggtatc gtttgaacac ggcattagtc
     5461 agggaagtca taacacagtc ctttcccgca attttctttt tctattactc ttggcctcct
     5521 ctagtacact ctatattttt ttatgcctcg gtaatgattt tcattttttt ttttccacct
     5581 agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa ttatacatta
     5641 tataaagtaa tgtgatttct tcgaagaata tactaaaaaa tgagcaggca agataaacga
     5701 aggcaaagat gacagagcag aaagccctag taaagcgtat tacaaatgaa accaagattc
     5761 agattgcgat ctctttaaag ggtggtcccc tagcgataga gcactcgatc ttcccagaaa
     5821 aagaggcaga agcagtagca gaacaggcca cacaatcgca agtgattaac gtccacacag
     5881 gtatagggtt tctggaccat atgatacatg ctctggccaa gcattccggc tggtcgctaa
     5941 tcgttgagtg cattggtgac ttacacatag acgaccatca caccactgaa gactgcggga
     6001 ttgctctcgg tcaagctttt aaagaggccc taggggccgt gcgtggagta aaaaggtttg
     6061 gatcaggatt tgcgcctttg gatgaggcac tttccagagc ggtggtagat ctttcgaaca
     6121 ggccgtacgc agttgtcgaa cttggtttgc aaagggagaa agtaggagat ctctcttgcg
     6181 agatgatccc gcattttctt gaaagctttg cagaggctag cagaattacc ctccacgttg
     6241 attgtctgcg aggcaagaat gatcatcacc gtagtgagag tgcgttcaag gctcttgcgg
     6301 ttgccataag agaagccacc tcgcccaatg gtaccaacga tgttccctcc accaaaggtg
     6361 ttcttatgta gtgacaccga ttatttaaag ctgcagcata cgatatatat acatgtgtat
     6421 atatgtatac ctatgaatgt cagtaagtat gtatacgaac agtatgatac tgaagatgac
     6481 aaggtaatgc atcattctat acgtgtcatt ctgaacgagg cgcgctttcc ttttttcttt
     6541 ttgctttttc tttttttttc tcttgaactc gacggatcta tgcggtgtga aataccgcac
     6601 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     6661 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     6721 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     6781 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     6841 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     6901 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     6961 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     7021 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     7081 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgcggtg cgggcctctt
     7141 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7201 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     7261 cactataggg cgaattgggt accgggcccc cccgagctca gttta
//
