LOCUS       sequence-309666-        8036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  M. magneticum/E. coli shuttle vector (pMGT and pUC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Furubayashi M, Wallace AK, Gonzalez LM, Jahnke JP, Hanrahan BM, 
            Payne AL, Stratis-Cullum DN, Gray MT, Liu H, Rhoads MK, Voigt CA
  TITLE     Genetic Tuning of Iron Oxide Nanoparticle Size, Shape, and Surface 
            Properties in Magnetospirillum magneticum
  JOURNAL   Adv. Funct. Mater. 2021, 31, 2004813
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv. Funct.
            Mater. 2021, 31, 2004813"
COMMENT     Sequence Label: pMGU
FEATURES             Location/Qualifiers
     source          1..8036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(14..100)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(186..229)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    232..253
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        254..288
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             332..1045
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1098..1192
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      3117..3126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4986..5002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4986..5002)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5010..5026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5034..5040,5041..5058,5059..5064))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5079..5100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5217..5234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5388..5976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5468..5487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6245..7039)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6356..6375)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6966..6985
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     oriT            7435..7544
                     /note="incP origin of transfer"
     CDS             7577..7948
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
       61 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaaagcgga
      121 taacaatttc acacaggagg ccgcctaggc cgcggccgcg cgaattcggc gtatcacgag
      181 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
      241 gccgcttcta gagtttacag ctagctcagt cctaggtatt atgctagcac tctagaaata
      301 attttgttta tctctcgagg aggtatacta gatgcgtaaa ggcgaagagc tgttcactgg
      361 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      421 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
      481 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
      541 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
      601 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc
      661 ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt
      721 taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt
      781 ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa
      841 cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga
      901 tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga
      961 tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac
     1021 gcatggtatg gatgaactgt acaaatgata agcttgcggc cgcgtcgtga ctgggaaaac
     1081 cctggcgact agtcttggac tcctgttgat agatccagta atgacctcag aactccatct
     1141 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt
     1201 ccccaataat tacgatttaa attggcggga tccaatcgct cgagcgcgag gtcacaattg
     1261 gagatgatcc gccagcgccg aagccatcat aactgcattg aataattcaa agtgcattga
     1321 ataaggcagg gtgcataagg cgcagccttt cgattaactc catttcctgc ggcgacaacg
     1381 tcgccgcttg cccccccgac gggggtcaag cgaggcacag ggacccgccg cctcttggcg
     1441 gtgggtggcc gcagcttgac gcccgaacac tccgccgccc aaccctgcct gctctcctct
     1501 gttcaatgaa attgaacgga ggagatgcct cccggcgagg gcctttcttt cttgcataac
     1561 gttcatgcta cgttccaacg gttataccgt atgagcgtcg aactgtataa tcgttgaaca
     1621 gtcgaaccat ccccccgcgc cagcgcgggg agcagggatg ggttcggggg ccggaagtta
     1681 ggcaaatcgc ggagccgtgg cgccctcggt acatccgcga aatcggttga cactttgccc
     1741 ccagaacggc tgcaacctac cgcttggcga cagccgcgtg acggtacttt tccgctgtgg
     1801 attcggacac gaatatctgt tcagcagcca gggcgcgggc gatctggcga cggccgaggc
     1861 cctgatgggc caattctgtg atccggtcta tgaccgcttg gctcatcggc acccgaggac
     1921 cgccgatacc agattggcgc acatcgccca tggtgggtgg aaccgaggga gccggagccg
     1981 gtgcagattt ctgcacagtt ggttcacgcc gtcgttctag cgcatcgaga cgctgcacga
     2041 tcacggcgag ctgatcgttg acgtattgtc gctcctcgga ctgcgcaatt gtgatcagaa
     2101 attcgaggag ctcggccacg gtgacgccgc gtcgaagggc catagcctgt atgtggtcgc
     2161 ggaccgcatc aggacagcga atcgtccatc tcgtgtctga cacattcccg gtttttgtct
     2221 gactcatcgt cgcccccaat gtctgacaga ttcgagaaag agtcataccg gctaacgcga
     2281 acgctgacaa cgaatgtctg acaaaatgat gattacgtca gacattgatg tctgcctaaa
     2341 tcgcaatagt gtctgacatg gtttttggcg ttccgtatat aaaatatgcg ccaaaatcta
     2401 gtgcgtcagc caaacggcaa acgcaaccag ttgacaccat ttccggtttt atgagactgt
     2461 gaaaagacca aaggccgcct tgcggcggcc taggtgattg tggtcccggc gaccaaggct
     2521 gcgaaaactt ggcggtccag cagcagagga agcccgtttg aaaggtccgg cttgcgccgg
     2581 aaccacctaa tgacaatagc aattcgccgc acagttttca acacccagcc cgccatgatt
     2641 cgcggcggcg ttgaacgttg aacaacagac gtctcaaaca gacacccgca aacgcgaacg
     2701 gtatcggttg caggatgcag ccgctgaact gctgccggat tatcgggtgc ggcattgcca
     2761 ccgctcaatt ggatgtggct gtggcgagtt ggtcagcgtt gttaccaatg ggcagtcggc
     2821 caatttcaaa ggcgtgcaga cctgcggctc ggtctggcac tgcccaatct gttcggcaaa
     2881 aatcagcaat gttcggcgcg gcgagctgaa cgagctgctg cgctgggggc gggagaggga
     2941 gctgatcccg ctgatggtga cgctgacagg ccatcacaat gcacggatga gcctcgctga
     3001 atgtctgacg gtgatgaaga aggccaagca gagccttcac cagtctggcc catggaagcg
     3061 ccatgttggc ccggtggtcg aagggcacgt cacggcaacg gaggtcaatc ggtcacgccg
     3121 ccatggctgg cacgtccatt ttcacgtcct gatgctgatt gaggccgcca gcgaggccga
     3181 agcgattgcg ctcgtgaccc gacacctcga acctgaatgg ctccggcagc ttgagcgcca
     3241 aggcagctgg ggcggcgaac atgcgttaga cgtccagggc gcagccgatg cggatcggta
     3301 tgtggccaag tggggcgcgg ctgaggaatt ggctctgtcg ggcgaaaagc gggcgcgcgg
     3361 cgacgccgaa attaagggtc aaacgccgtg ggaattgttg gccgctgcgg ctgatggtga
     3421 cgtccaggct ggccgcgatt ttgtggagta cgcgaccctg tttcatggca aacgccagct
     3481 cgtttggtcg cgaggactga aagagcgtgt cggcctcaag gtggtggaag acgacgaggc
     3541 cgccggcgag ctggaggatg tcgcagatag cgccatcaac gaagtggccg tgttgccgag
     3601 gccgacttgg cgtcacatcg tccggcgcag gctccgcgcg gatctgctgg ccgccgctcg
     3661 tgatgacgga ttcgatggcg tcgcgcggct gctcaatgag catggcatcg gcggagctat
     3721 gcccggcggc tggcgaccaa tgcttagcga atataccgag catattgcga tggctgcgtg
     3781 actcttttgg cccaccggcc tgagggaaat ttgcctcgca gagctcacga taatttgaca
     3841 agggccgtag gcccgcaggc aaattgtcta gtgagtgtac aattttcaaa ttcgaaggtt
     3901 attgtagagc tgacgctctc aaggtgatga cgcctatggc tgacgattct ggcgtccggc
     3961 ggattgtttg tcggattagc aaaatccaca cgatgggcgt gctgaaggcc cggtcgaatc
     4021 acaatctccg aacgccaggt catgaccagc tcgcacatgt tgaccaggcc aaatccgctc
     4081 agaatgaaat tctgattggc accggcgacg ccatgctcga tgtgcaatca gcattgaaat
     4141 cgcggctggt cggcaacatt cgaaaaaatg gcgttatcgc agtggaaatg gtgatgacag
     4201 cccgggcgga ctggttcgct gccgatcccg cgcgagctgc cgcatttaag cagcgggtgc
     4261 tggacgaggc gaatgccaga tatggggcga atctcgtaac cgcagtgtgg cacatggatg
     4321 aagaggctcc ccatctgcat ctgtatatcg tgccgatgga ccaacggggc aaactgaact
     4381 gccgcgcgca gttctcgacg atggcgtcgc tgaaacaact ccaggattgg gctggcgtgg
     4441 tcggcaatcc actcggcctc gaacgcggac ggccaaaagt ggagtttatt gtcgatggcg
     4501 atgagccgcc ggagaaccaa agcccggcgg aatatcgagc tcaaaccaaa cgcgacgctg
     4561 aagcggcagc cgccgcgcgg atcgctgccg agcgtgatgc tgccgaggct gcgcgactgc
     4621 tgattgaggc tcaggagcag gccgccgcga tccgcgcggc ccaggctgaa attatggcag
     4681 agcgtcgcca gctcaatgag gataaggcga gttttgctga gacgctgcgt agccacacga
     4741 gggcgatggg cctggcgttt cgttcatggc tggccaattt cgtcggccgc ggccagatgc
     4801 aggctgatgg agggccattg ctgaccttta aagccgcatg ccccgccagt aagcgagagg
     4861 agctacagtc cgagccagct aatgcgtacg cgcgcctggt ggtcgctcat atgcctgatc
     4921 gtgacgctct tgccgtgctc gaaaccaaaa gcgcggaggc caaggaatag cttggcgtaa
     4981 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5041 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5101 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5161 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5581 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5821 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6061 aaaaggatct tcacctagat ccagaactcc agcatgagat ccccgcgctg gaggatcatc
     6121 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     6181 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     6241 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     6301 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     6361 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     6421 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     6481 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     6541 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     6601 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     6661 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     6721 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     6781 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     6841 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     6901 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     6961 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     7021 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
     7081 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     7141 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     7201 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     7261 cgttttccct tgtccagata gcccagggga agcgtggcgc ttttccgctg cataaccctg
     7321 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     7381 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     7441 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     7501 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac
     7561 agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat
     7621 caaggtgtac tgccttccag acgaacgaag agcgattgag gaaaaggcgg cggcggccgg
     7681 catgagcctg tcggcctacc tgctggccgt cggccagggc tacaaaatca cgggcgtcgt
     7741 ggactatgag cacgtccgcg agctggcccg catcaatggc gacctgggcc gcctgggcgg
     7801 cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg cggttcggtg atgccacgat
     7861 cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag cttggcaagg tcatgatggg
     7921 cgtggtccgc ccgagggcag agccatgact tttttagccg ctaaaacggc cggggggtgc
     7981 gcgtgattgc caagcacgtc cccatgcgct ccatacagtt agggcgcgcc cagctg
//
