LOCUS       sequence-309670-        8891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AHL-inducible sfGFP expression on M. magneticum/E. coli shuttle 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Furubayashi M, Wallace AK, Gonzalez LM, Jahnke JP, Hanrahan BM, 
            Payne AL, Stratis-Cullum DN, Gray MT, Liu H, Rhoads MK, Voigt CA
  TITLE     Genetic Tuning of Iron Oxide Nanoparticle Size, Shape, and Surface 
            Properties in Magnetospirillum magneticum
  JOURNAL   Adv. Funct. Mater. 2021, 31, 2004813
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv. Funct.
            Mater. 2021, 31, 2004813"
COMMENT     Sequence Label: pMGA-plux-sfGFP
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             92..805
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      858..952
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      2877..2886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      complement(4791..5336)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4871..4890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5539..6333)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5650..5669)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6260..6279
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     oriT            6729..6838
                     /note="incP origin of transfer"
     CDS             6871..7242
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     terminator      complement(7344..7430)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8440..8459
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8479..8556)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     8748..8766
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        join(8869..8891,1..27)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa tctagaaata
       61 attttgttta tctctcgagg aggtatacta gatgcgtaaa ggcgaagagc tgttcactgg
      121 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      181 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
      241 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
      301 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
      361 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc
      421 ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt
      481 taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt
      541 ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa
      601 cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga
      661 tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga
      721 tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac
      781 gcatggtatg gatgaactgt acaaatgata agcttgcggc cgcgtcgtga ctgggaaaac
      841 cctggcgact agtcttggac tcctgttgat agatccagta atgacctcag aactccatct
      901 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt
      961 ccccaataat tacgatttaa attggcggga tccaatcgct cgagcgcgag gtcacaattg
     1021 gagatgatcc gccagcgccg aagccatcat aactgcattg aataattcaa agtgcattga
     1081 ataaggcagg gtgcataagg cgcagccttt cgattaactc catttcctgc ggcgacaacg
     1141 tcgccgcttg cccccccgac gggggtcaag cgaggcacag ggacccgccg cctcttggcg
     1201 gtgggtggcc gcagcttgac gcccgaacac tccgccgccc aaccctgcct gctctcctct
     1261 gttcaatgaa attgaacgga ggagatgcct cccggcgagg gcctttcttt cttgcataac
     1321 gttcatgcta cgttccaacg gttataccgt atgagcgtcg aactgtataa tcgttgaaca
     1381 gtcgaaccat ccccccgcgc cagcgcgggg agcagggatg ggttcggggg ccggaagtta
     1441 ggcaaatcgc ggagccgtgg cgccctcggt acatccgcga aatcggttga cactttgccc
     1501 ccagaacggc tgcaacctac cgcttggcga cagccgcgtg acggtacttt tccgctgtgg
     1561 attcggacac gaatatctgt tcagcagcca gggcgcgggc gatctggcga cggccgaggc
     1621 cctgatgggc caattctgtg atccggtcta tgaccgcttg gctcatcggc acccgaggac
     1681 cgccgatacc agattggcgc acatcgccca tggtgggtgg aaccgaggga gccggagccg
     1741 gtgcagattt ctgcacagtt ggttcacgcc gtcgttctag cgcatcgaga cgctgcacga
     1801 tcacggcgag ctgatcgttg acgtattgtc gctcctcgga ctgcgcaatt gtgatcagaa
     1861 attcgaggag ctcggccacg gtgacgccgc gtcgaagggc catagcctgt atgtggtcgc
     1921 ggaccgcatc aggacagcga atcgtccatc tcgtgtctga cacattcccg gtttttgtct
     1981 gactcatcgt cgcccccaat gtctgacaga ttcgagaaag agtcataccg gctaacgcga
     2041 acgctgacaa cgaatgtctg acaaaatgat gattacgtca gacattgatg tctgcctaaa
     2101 tcgcaatagt gtctgacatg gtttttggcg ttccgtatat aaaatatgcg ccaaaatcta
     2161 gtgcgtcagc caaacggcaa acgcaaccag ttgacaccat ttccggtttt atgagactgt
     2221 gaaaagacca aaggccgcct tgcggcggcc taggtgattg tggtcccggc gaccaaggct
     2281 gcgaaaactt ggcggtccag cagcagagga agcccgtttg aaaggtccgg cttgcgccgg
     2341 aaccacctaa tgacaatagc aattcgccgc acagttttca acacccagcc cgccatgatt
     2401 cgcggcggcg ttgaacgttg aacaacagac gtctcaaaca gacacccgca aacgcgaacg
     2461 gtatcggttg caggatgcag ccgctgaact gctgccggat tatcgggtgc ggcattgcca
     2521 ccgctcaatt ggatgtggct gtggcgagtt ggtcagcgtt gttaccaatg ggcagtcggc
     2581 caatttcaaa ggcgtgcaga cctgcggctc ggtctggcac tgcccaatct gttcggcaaa
     2641 aatcagcaat gttcggcgcg gcgagctgaa cgagctgctg cgctgggggc gggagaggga
     2701 gctgatcccg ctgatggtga cgctgacagg ccatcacaat gcacggatga gcctcgctga
     2761 atgtctgacg gtgatgaaga aggccaagca gagccttcac cagtctggcc catggaagcg
     2821 ccatgttggc ccggtggtcg aagggcacgt cacggcaacg gaggtcaatc ggtcacgccg
     2881 ccatggctgg cacgtccatt ttcacgtcct gatgctgatt gaggccgcca gcgaggccga
     2941 agcgattgcg ctcgtgaccc gacacctcga acctgaatgg ctccggcagc ttgagcgcca
     3001 aggcagctgg ggcggcgaac atgcgttaga cgtccagggc gcagccgatg cggatcggta
     3061 tgtggccaag tggggcgcgg ctgaggaatt ggctctgtcg ggcgaaaagc gggcgcgcgg
     3121 cgacgccgaa attaagggtc aaacgccgtg ggaattgttg gccgctgcgg ctgatggtga
     3181 cgtccaggct ggccgcgatt ttgtggagta cgcgaccctg tttcatggca aacgccagct
     3241 cgtttggtcg cgaggactga aagagcgtgt cggcctcaag gtggtggaag acgacgaggc
     3301 cgccggcgag ctggaggatg tcgcagatag cgccatcaac gaagtggccg tgttgccgag
     3361 gccgacttgg cgtcacatcg tccggcgcag gctccgcgcg gatctgctgg ccgccgctcg
     3421 tgatgacgga ttcgatggcg tcgcgcggct gctcaatgag catggcatcg gcggagctat
     3481 gcccggcggc tggcgaccaa tgcttagcga atataccgag catattgcga tggctgcgtg
     3541 actcttttgg cccaccggcc tgagggaaat ttgcctcgca gagctcacga taatttgaca
     3601 agggccgtag gcccgcaggc aaattgtcta gtgagtgtac aattttcaaa ttcgaaggtt
     3661 attgtagagc tgacgctctc aaggtgatga cgcctatggc tgacgattct ggcgtccggc
     3721 ggattgtttg tcggattagc aaaatccaca cgatgggcgt gctgaaggcc cggtcgaatc
     3781 acaatctccg aacgccaggt catgaccagc tcgcacatgt tgaccaggcc aaatccgctc
     3841 agaatgaaat tctgattggc accggcgacg ccatgctcga tgtgcaatca gcattgaaat
     3901 cgcggctggt cggcaacatt cgaaaaaatg gcgttatcgc agtggaaatg gtgatgacag
     3961 cccgggcgga ctggttcgct gccgatcccg cgcgagctgc cgcatttaag cagcgggtgc
     4021 tggacgaggc gaatgccaga tatggggcga atctcgtaac cgcagtgtgg cacatggatg
     4081 aagaggctcc ccatctgcat ctgtatatcg tgccgatgga ccaacggggc aaactgaact
     4141 gccgcgcgca gttctcgacg atggcgtcgc tgaaacaact ccaggattgg gctggcgtgg
     4201 tcggcaatcc actcggcctc gaacgcggac ggccaaaagt ggagtttatt gtcgatggcg
     4261 atgagccgcc ggagaaccaa agcccggcgg aatatcgagc tcaaaccaaa cgcgacgctg
     4321 aagcggcagc cgccgcgcgg atcgctgccg agcgtgatgc tgccgaggct gcgcgactgc
     4381 tgattgaggc tcaggagcag gccgccgcga tccgcgcggc ccaggctgaa attatggcag
     4441 agcgtcgcca gctcaatgag gataaggcga gttttgctga gacgctgcgt agccacacga
     4501 gggcgatggg cctggcgttt cgttcatggc tggccaattt cgtcggccgc ggccagatgc
     4561 aggctgatgg agggccattg ctgaccttta aagccgcatg ccccgccagt aagcgagagg
     4621 agctacagtc cgagccagct aatgcgtacg cgcgcctggt ggtcgctcat atgcctgatc
     4681 gtgacgctct tgccgtgctc gaaaccaaaa gcgcggaggc caaggaatag cttggcgtaa
     4741 tcatgggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     4801 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     4861 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     4921 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     4981 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     5041 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     5101 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     5161 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     5221 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     5281 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     5341 atcatcttat taatcagata aaatatttca agatttagaa ctccagcatg agatccccgc
     5401 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     5461 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     5521 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     5581 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     5641 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     5701 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     5761 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     5821 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     5881 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     5941 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     6001 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     6061 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     6121 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     6181 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     6241 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     6301 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     6361 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     6421 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     6481 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     6541 tctctttgcg cttgcgtttt cccttgtcca gatagcccag gggaagcgtg gcgcttttcc
     6601 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     6661 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     6721 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     6781 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct
     6841 accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag
     6901 ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag
     6961 gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa
     7021 atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg
     7081 ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc
     7141 ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc
     7201 aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa
     7261 cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatac agttagggcg
     7321 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     7381 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctttaa
     7441 ttaaagcgga taacaatttc agaattcccg gcgtagagga tctgctcatg tttgacagct
     7501 tatcatcgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg
     7561 ctactccctc gagccgtcaa ttgtctgatt cgttaccaat tattaatttt taaagtatgg
     7621 gcaatcaatt gctcctgtta aaattgcttt agaaatactt tggcagcggt ttgttgtatt
     7681 gagtttcatt tgcgcattgg ttaaatggaa agtgacagta cgctcactgc aacctaatat
     7741 ttttgaaata tcccaagagc tttttccttc gcatgcccac gctaaacatt ctttttctct
     7801 tttggttaaa tcgttgtttg atttattatt tgctatattt atttttcgat aattatcaac
     7861 tagagaagga acaattaatg gtatgttcat acacgcatgt aaaaataaac tatctatata
     7921 gttgtctttt tctgaatgtg caaaactaag cattccgaag ccattgttag ccgtatgaat
     7981 agggaaacta aacccagtga taagacctga tgttttcgct tctttaatta catttggaga
     8041 ttttttattt acagcattgt tttcaaatat attccaatta attggtgaat gattggagtt
     8101 agaataatct actataggat catattttat taaattagcg tcatcataat attgcctcca
     8161 ttttttaggg taattatcta ggattgaaat atcagattta accatagaat gaggataaat
     8221 gatcgcgagt aaataatatt cacaatgtac cattttagtc atatcagata agcattgatt
     8281 aatatcatta ttgcttctac aagctttaat tttattaatt attctgtatg tgtcgtcggc
     8341 atttatgttt ttcatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     8401 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     8461 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     8521 cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttcgcc
     8581 ttcgcgcgcg aattgcaggt accatttatc agggttattg tctcatgagc ggatacatat
     8641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8701 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8761 cgaggccctt tcgtcttcac ctcgagaaaa tttatcaaaa agagtacctg taggatcgta
     8821 caggtttacg caagaaaatg gtttgttact ttcgaataaa atcgatagct gtcaccggat
     8881 gtgctttccg g
//
