LOCUS       sequence-309672-        6289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  M. magneticum/E. coli shuttle vector (partial sequence of pMGT and 
            p15A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Furubayashi M, Wallace AK, Gonzalez LM, Jahnke JP, Hanrahan BM, 
            Payne AL, Stratis-Cullum DN, Gray MT, Liu H, Rhoads MK, Voigt CA
  TITLE     Genetic Tuning of Iron Oxide Nanoparticle Size, Shape, and Surface 
            Properties in Magnetospirillum magneticum
  JOURNAL   Adv. Funct. Mater. 2021, 31, 2004813
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv. Funct.
            Mater. 2021, 31, 2004813"
COMMENT     Sequence Label: pMGB
FEATURES             Location/Qualifiers
     source          1..6289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2016..2025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      complement(2750..3295)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2830..2849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3498..4292)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3609..3628)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4219..4238
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     oriT            4688..4797
                     /note="incP origin of transfer"
     CDS             4830..5201
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     terminator      complement(5303..5389)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5437..5471
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             5513..6226
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      join(6286..6289,1..91)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
       61 ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa
      121 ttggcgggat ccaatcgctc gagcgcgagg tcacaattgg agatgatccg ccagcgccga
      181 agccatcata actgcattga ataattcaaa gtgcattgaa taaggcaggg tgcataaggc
      241 gcagcctttc gattaactcc atttcctgcg gcgacaacgt cgccgcttgc ccccccgacg
      301 ggggtcaagc gaggcacagg gacccgccgc ctcttggcgg tgggtggccg cagcttgacg
      361 cccgaacact ccgccgccca accctgcctg ctctcctctg ttcaatgaaa ttgaacggag
      421 gagatgcctc ccggcgaggg cctttctttc ttgcataacg ttcatgctac gttccaacgg
      481 ttataccgta tgagcgtcga actgtataat cgttgaacag tcgaaccatc cccccgcgcc
      541 agcgcgggga gcagggatgg gttcgggggc cggaagttag gcaaatcgcg gagccgtggc
      601 gccctcggta catccgcgaa atcggttgac actttgcccc cagaacggct gcaacctacc
      661 gcttggcgac agccgcgtga cggtactttt ccgctgtgga ttcggacacg aatatctgtt
      721 cagcagccag ggcgcgggcg atctggcgac ggccgaggcc ctgatgggcc aattctgtga
      781 tccggtctat gaccgcttgg ctcatcggca cccgaggacc gccgatacca gattggcgca
      841 catcgccaat ggtgggtgga accgagggag ccggagccgg tgcagatttc tgcacagttg
      901 gttcacgccg tcgttctagc gcatcgagac gctgcacgat cacggcgagc tgatcgttga
      961 cgtattgtcg ctcctcggac tgcgcaattg tgatcagaaa ttcgaggagc tcggccacgg
     1021 tgacgccgcg tcgaagggcc atagcctgta tgtggtcgcg gaccgcatca ggacagcgaa
     1081 tcgtccatct cgtgtctgac acattcccgg tttttgtctg actcatcgtc gcccccaatg
     1141 tctgacagat tcgagaaaga gtcataccgg ctaacgcgaa cgctgacaac gaatgtctga
     1201 caaaatgatg attacgtcag acattgatgt ctgcctaaat cgcaatagtg tctgacatgg
     1261 tttttggcgt tccgtatata aaatatgcgc caaaatctag tgcgtcagcc aaacggcaaa
     1321 cgcaaacagt tgacaccatt tccggtttta tgagactgtg aaaagaccaa aggccgcctt
     1381 gcggcggcct aggtgattgt ggtcccggcg accaaggctg cgaaaacttg gcggtccagc
     1441 agcagaggaa gcccgtttga aaggtccggc ttgcgccgga accacctaat gacaatagca
     1501 attcgccgca cagttttcaa cacccagccc gccatgattc gcggcggcgt tgaacgttga
     1561 acaacagacg tctcaaacag acacccgcaa acgcgaacgg tatcggttgc aggatgcagc
     1621 cgctgaactg ctgccggatt atcgggtgcg gcattgccac cgctcaattg gatgtggctg
     1681 tggcgagttg gtcagcgttg ttaccaatgg gcagtcggcc aatttcaaag gcgtgcagac
     1741 ctgcggctcg gtctggcact gcccaatctg ttcggcaaaa atcagcaatg ttcggcgcgg
     1801 cgagctgaac gagctgctgc gctgggggcg ggagagggag ctgatcccgc tgatggtgac
     1861 gctgacaggc catcacaatg cacggatgag cctcgctgaa tgtctgacgg tgatgaagaa
     1921 ggccaagcag agccttcacc agtctggccc atggaagcgc catgttggcc cggtggtcga
     1981 agggcacgtc acggcaacgg aggtcaatcg gtcacgccgc catggctggc acgtccattt
     2041 tcacgtcctg atgctgattg aggccgccag cgaggccgaa gcgattgcgc tcgtgacccg
     2101 acacctcgaa cctgaatggc tccggcagct tgagcgccaa ggcagctggg gcggcgaaca
     2161 tgcgttagac gtccagggcg cagccgatgc ggatcggtat gtggccaagt ggggcgcggc
     2221 tgaggaattg gctctgtcgg gcgaaaagcg ggcgcgcggc gacgccgaaa ttaagggtca
     2281 aacgccgtgg gaattgttgg ccgctgcggc tgatggtgac gtccaggctg gccgcgattt
     2341 tgtggagtac gcgaccctgt ttcatggcaa acgccagctc gtttggtcgc gaggactgaa
     2401 agagcgtgtc ggcctcaagg tggtggaaga cgacgaggcc gccggcgagc tggaggatgt
     2461 cgcagatagc gccatcaacg aagtggccgt gttgccgagg ccgacttggc gtcacatcgt
     2521 ccggcgcagg ctccgcgcgg atctgctggc cgccgctcgt gatgacggat tcgatggcgt
     2581 cgcgcggctg ctcaatgagc atggcatcgg cggagctatg cccggcggct ggcgaccaat
     2641 gcttagcgaa tataccgagc atattgcgat ggctgcgtga ctcttttggc ccaccggcct
     2701 gagggaaatt tgcctcgcga ttaagtgaga gggccgcggc aaagccgttt ttccataggc
     2761 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     2821 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
     2881 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     2941 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     3001 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     3061 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     3121 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     3181 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     3241 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     3301 tcatcttatt aatcagataa aatatttcaa gatttagaac tccagcatga gatccccgcg
     3361 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     3421 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     3481 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     3541 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     3601 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     3661 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     3721 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     3781 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     3841 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     3901 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     3961 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     4021 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     4081 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     4141 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     4201 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     4261 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     4321 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     4381 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     4441 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     4501 ctctttgcgc ttgcgttttc ccttgtccag atagcccagg ggaagcgtgg cgcttttccg
     4561 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     4621 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     4681 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     4741 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     4801 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     4861 gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg
     4921 cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa
     4981 tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg
     5041 gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg
     5101 gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca
     5161 aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac
     5221 ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccataca gttagggcgc
     5281 gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     5341 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat
     5401 taaagcggat aacaatttca gaattctcat gagaccttga cagctagctc agtcctaggt
     5461 ataatgctag ctctagaaat aattttgttt atctctcgag gaggtatact agatgcgtaa
     5521 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
     5581 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
     5641 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
     5701 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
     5761 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
     5821 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
     5881 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
     5941 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
     6001 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
     6061 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
     6121 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
     6181 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaatgat ggtctcaaag
     6241 cttgcggccg cgtcgtgact gggaaaaccc tggcgactag tcttggact
//
